Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WJ12_RS14035 Genome accession   NZ_CP013398
Coordinates   3104188..3105693 (+) Length   501 a.a.
NCBI ID   WP_034189274.1    Uniprot ID   -
Organism   Burkholderia seminalis strain FL-5-4-10-S1-D7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3099188..3110693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ12_RS14010 (WJ12_13995) kdsD 3100420..3101403 (+) 984 WP_059558722.1 arabinose 5-phosphate isomerase KdsD -
  WJ12_RS14015 (WJ12_14000) - 3101403..3101939 (+) 537 WP_034189277.1 KdsC family phosphatase -
  WJ12_RS14020 (WJ12_14005) lptC 3101943..3102557 (+) 615 WP_034189276.1 LPS export ABC transporter periplasmic protein LptC -
  WJ12_RS14025 (WJ12_14010) lptA 3102578..3103243 (+) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  WJ12_RS14030 (WJ12_14015) lptB 3103240..3104016 (+) 777 WP_034189275.1 LPS export ABC transporter ATP-binding protein -
  WJ12_RS14035 (WJ12_14020) rpoN/rpoN1 3104188..3105693 (+) 1506 WP_034189274.1 RNA polymerase factor sigma-54 Machinery gene
  WJ12_RS14040 (WJ12_14025) hpf 3105852..3106211 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WJ12_RS14045 (WJ12_14030) - 3106406..3106918 (+) 513 WP_049034209.1 PTS sugar transporter subunit IIA -
  WJ12_RS14050 (WJ12_14035) hprK 3107048..3108016 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  WJ12_RS14055 (WJ12_14040) rapZ 3108092..3109000 (+) 909 WP_034189273.1 RNase adapter RapZ -
  WJ12_RS14060 (WJ12_14045) - 3109028..3109663 (+) 636 WP_059558724.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54721.84 Da        Isoelectric Point: 4.8711

>NTDB_id=162480 WJ12_RS14035 WP_034189274.1 3104188..3105693(+) (rpoN/rpoN1) [Burkholderia seminalis strain FL-5-4-10-S1-D7]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAS
ANGSSTSSDRAERDEPAGADDYDGYGSGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPPGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162480 WJ12_RS14035 WP_034189274.1 3104188..3105693(+) (rpoN/rpoN1) [Burkholderia seminalis strain FL-5-4-10-S1-D7]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCCACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCGCAGAACACCGAGCCGGCCTCG
GCAAACGGCAGCAGCACGTCCAGCGATCGCGCGGAGCGCGACGAGCCGGCCGGTGCCGACGACTACGACGGTTACGGTTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGC
CCGGGGTCGGCGCACGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTCGAACTGCTCGCGGCCCGCGATTTCACACGATTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGGTGGCTCGCGGAACTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGTTTCGACACGATCCTGCGAGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTGGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment