Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WJ02_RS12885 Genome accession   NZ_CP013393
Coordinates   2829415..2830920 (+) Length   501 a.a.
NCBI ID   WP_059453957.1    Uniprot ID   -
Organism   Burkholderia vietnamiensis strain FL-2-3-30-S1-D0     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2824415..2835920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ02_RS12860 (WJ02_12845) kdsD 2825656..2826639 (+) 984 WP_059495299.1 arabinose 5-phosphate isomerase KdsD -
  WJ02_RS12865 (WJ02_12850) - 2826639..2827175 (+) 537 WP_059453956.1 KdsC family phosphatase -
  WJ02_RS12870 (WJ02_12855) lptC 2827179..2827793 (+) 615 WP_011886053.1 LPS export ABC transporter periplasmic protein LptC -
  WJ02_RS12875 (WJ02_12860) lptA 2827814..2828479 (+) 666 WP_011886054.1 lipopolysaccharide transport periplasmic protein LptA -
  WJ02_RS12880 (WJ02_12865) lptB 2828476..2829252 (+) 777 WP_011886055.1 LPS export ABC transporter ATP-binding protein -
  WJ02_RS12885 (WJ02_12870) rpoN/rpoN1 2829415..2830920 (+) 1506 WP_059453957.1 RNA polymerase factor sigma-54 Machinery gene
  WJ02_RS12890 (WJ02_12875) hpf 2831079..2831438 (+) 360 WP_011886057.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WJ02_RS12895 (WJ02_12880) - 2831633..2832145 (+) 513 WP_011886058.1 PTS sugar transporter subunit IIA -
  WJ02_RS12900 (WJ02_12885) hprK 2832275..2833243 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  WJ02_RS12905 (WJ02_12890) rapZ 2833319..2834227 (+) 909 WP_059453958.1 RNase adapter RapZ -
  WJ02_RS12910 (WJ02_12895) - 2834255..2834893 (+) 639 WP_011886060.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54724.85 Da        Isoelectric Point: 4.8247

>NTDB_id=162465 WJ02_RS12885 WP_059453957.1 2829415..2830920(+) (rpoN/rpoN1) [Burkholderia vietnamiensis strain FL-2-3-30-S1-D0]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPAPNGESGS
MNGSGQSSERAERDEPAATDEYDGYGSGDASDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLREHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKQLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162465 WJ02_RS12885 WP_059453957.1 2829415..2830920(+) (rpoN/rpoN1) [Burkholderia vietnamiensis strain FL-2-3-30-S1-D0]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACACCTTGCGTTGACGCCGCAGCTGCAGCAGTCGATTCGGCTTTTGCA
GCTGTCGACGCTCGAGCTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTGGAAAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCAGCCGACGGATCGCTGATCGCGCAGACGCCGCCCGCCCCGAACGGCGAGTCCGGCAGC
ATGAACGGCAGCGGCCAGTCGAGCGAGCGCGCCGAACGCGACGAGCCGGCCGCCACCGACGAATACGACGGCTATGGCTC
GGGCGACGCGAGCGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCGGACGACGACGATCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCCACATCGCTGCGCGAGCATCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGACGAAGTGCT
GGCCGACCTGCCGCCCGAACTCGAAGTCGACGGCGACGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAACTGCTGCGGCTCGATCCGTCCCCGACCCGCACGCTC
GCGCTCGAAATCGTCTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCGGCCTACGGCAAGGCCGAGG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAGGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATTCTGCGCAACAGTCGCGGCGATCCGTCGACCGGATCGCTGAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAACTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGTAAGTACATGCTGACCCCGTTCGGGACGCTGGAGTTCAAGTACTT
CTTCGGATCGCACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAGTCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTCGCGCGC
CGCACGGTCGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAATCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.031

100

0.697

Multiple sequence alignment