Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WI95_RS15115 Genome accession   NZ_CP013390
Coordinates   3169063..3170568 (+) Length   501 a.a.
NCBI ID   WP_122474347.1    Uniprot ID   -
Organism   Burkholderia contaminans strain FL-1-2-30-S1-D0     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3164063..3175568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WI95_RS15090 (WI95_14380) kdsD 3165307..3166290 (+) 984 WP_122474344.1 arabinose 5-phosphate isomerase KdsD -
  WI95_RS15095 (WI95_14385) - 3166290..3166826 (+) 537 WP_122474345.1 KdsC family phosphatase -
  WI95_RS15100 (WI95_14390) lptC 3166830..3167444 (+) 615 WP_046544392.1 LPS export ABC transporter periplasmic protein LptC -
  WI95_RS15105 (WI95_14395) lptA 3167465..3168130 (+) 666 WP_122474346.1 lipopolysaccharide transport periplasmic protein LptA -
  WI95_RS15110 (WI95_14400) lptB 3168127..3168903 (+) 777 WP_046544391.1 LPS export ABC transporter ATP-binding protein -
  WI95_RS15115 (WI95_14405) rpoN/rpoN1 3169063..3170568 (+) 1506 WP_122474347.1 RNA polymerase factor sigma-54 Machinery gene
  WI95_RS15120 (WI95_14410) hpf 3170727..3171086 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WI95_RS15125 (WI95_14415) - 3171255..3171794 (+) 540 WP_046544389.1 PTS sugar transporter subunit IIA -
  WI95_RS15130 (WI95_14420) hprK 3171924..3172892 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  WI95_RS15135 (WI95_14425) rapZ 3172968..3173876 (+) 909 WP_046544388.1 RNase adapter RapZ -
  WI95_RS15140 (WI95_14430) - 3173904..3174539 (+) 636 WP_039346030.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54725.83 Da        Isoelectric Point: 4.8740

>NTDB_id=162450 WI95_RS15115 WP_122474347.1 3169063..3170568(+) (rpoN/rpoN1) [Burkholderia contaminans strain FL-1-2-30-S1-D0]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
ANGSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162450 WI95_RS15115 WP_122474347.1 3169063..3170568(+) (rpoN/rpoN1) [Burkholderia contaminans strain FL-1-2-30-S1-D0]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCTGCGGACGGATCGCTGATCGCGCAGACGCCTCCCGCGCAGAACACCGAGCCCACTGCG
GCAAACGGTAGCAGCTCGTCGAGCGATCGTGCCGAGCGTGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCTCAAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGATGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGTGCCCGTTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTTTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCTTCGTCGACAGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.577

100

0.693

Multiple sequence alignment