Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WS71_RS32700 Genome accession   NZ_CP013389
Coordinates   3965039..3966550 (+) Length   503 a.a.
NCBI ID   WP_066495348.1    Uniprot ID   -
Organism   Burkholderia mayonis strain BDU8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3960039..3971550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WS71_RS32675 (WS71_29960) kdsD 3961273..3962256 (+) 984 WP_066495337.1 arabinose 5-phosphate isomerase KdsD -
  WS71_RS32680 (WS71_29965) - 3962256..3962795 (+) 540 WP_066495339.1 KdsC family phosphatase -
  WS71_RS32685 (WS71_29970) lptC 3962800..3963414 (+) 615 WP_066495341.1 LPS export ABC transporter periplasmic protein LptC -
  WS71_RS32690 (WS71_29975) lptA 3963433..3964098 (+) 666 WP_066495344.1 lipopolysaccharide transport periplasmic protein LptA -
  WS71_RS32695 (WS71_29980) lptB 3964095..3964871 (+) 777 WP_066495346.1 LPS export ABC transporter ATP-binding protein -
  WS71_RS32700 (WS71_29985) rpoN/rpoN1 3965039..3966550 (+) 1512 WP_066495348.1 RNA polymerase factor sigma-54 Machinery gene
  WS71_RS32710 (WS71_29990) hpf 3966703..3967062 (+) 360 WP_066495350.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WS71_RS32715 (WS71_29995) ptsN 3967298..3967807 (+) 510 WP_025989646.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WS71_RS32720 (WS71_30000) hprK 3967931..3968899 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  WS71_RS32725 (WS71_30005) rapZ 3968974..3969867 (+) 894 WP_066495352.1 RNase adapter RapZ -
  WS71_RS32730 (WS71_30010) - 3969894..3970526 (+) 633 WP_066495354.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55375.55 Da        Isoelectric Point: 4.9394

>NTDB_id=162435 WS71_RS32700 WP_066495348.1 3965039..3966550(+) (rpoN/rpoN1) [Burkholderia mayonis strain BDU8]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDDWIASPLRLAADGSVIAQTPASSAPEPMQ
GGTSNGTSATNERTERDEPAGVDEYDSYSADSGDASQWNLDDFGRSPSASDDDDLPPMQIHETATSLRDHLAAQLRVTQA
SPRDRALVMFLIESLDDDGYLTASLEEVLTDLPAELEVDLDELNAALALLHSFDPAGVGARSPSECLKLQLLRLDPSPTR
SLALDIVSQHLELLAARDFTRLRKQLKASDDELRNAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTGQNWQAELNPEV
VPRLRINHLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQRNFFAHGEIAMRPLVLREIADT
LGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=162435 WS71_RS32700 WP_066495348.1 3965039..3966550(+) (rpoN/rpoN1) [Burkholderia mayonis strain BDU8]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTCGCGCTCACACCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCGGTCGCGCAGAATCCGCTTCTCGAAAACGAAGACGACTGGA
TCGCAAGCCCGCTGCGCCTCGCGGCGGACGGCTCGGTGATCGCGCAGACGCCCGCATCGTCGGCGCCCGAGCCGATGCAA
GGCGGCACATCGAACGGCACGAGTGCGACGAATGAGCGCACCGAGCGCGACGAGCCTGCGGGCGTCGACGAATACGACAG
CTATTCGGCCGACAGCGGCGATGCGAGCCAGTGGAACCTCGACGATTTCGGCCGCTCGCCCTCCGCCTCGGACGACGACG
ATCTGCCGCCGATGCAGATCCACGAAACGGCGACGTCGCTGCGCGACCATCTCGCCGCGCAACTGCGCGTCACGCAAGCG
AGCCCGCGCGATCGCGCGCTGGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGAAGA
AGTTCTCACCGATCTGCCGGCGGAGCTCGAGGTCGACCTCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCG
ATCCGGCAGGCGTCGGCGCGCGCTCCCCATCCGAGTGCCTGAAGCTGCAGTTGCTGCGGCTCGATCCGTCCCCGACGCGC
TCGCTCGCGCTCGACATCGTGTCGCAGCATCTCGAACTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAA
GGCGTCCGACGACGAGCTGCGCAACGCGCACGCACTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGG
CCGAAGCGGATTACGTGGTGCCCGACATCATCGTAAAGAAGACGGGTCAGAACTGGCAGGCGGAACTCAACCCGGAAGTC
GTGCCGCGGCTGCGGATCAACCATCTGTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCACGGGTTCGCTCAA
GCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGATTCGAGACGATCCTGCGCGTCGCGCAAGCGA
TTGTCGAACGTCAGAGGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACG
CTGGGCCTACACGAATCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAA
GTACTTCTTCGGATCGCACGTTTCGACAGACACCGGAGGTGCCGCGTCGTCGACGGCAATCCGCGCCCTCATCAAACAAC
TGATAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGATAGTCGCATAGCCGAACTGCTAGCGGAACAAGGGTTCGTGGTC
GCGCGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-330)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.452

100

0.686

Multiple sequence alignment