Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WS70_RS16140 Genome accession   NZ_CP013386
Coordinates   3359083..3360591 (+) Length   502 a.a.
NCBI ID   WP_059596767.1    Uniprot ID   A0A1B4FGZ6
Organism   Burkholderia mayonis strain BDU6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3354083..3365591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WS70_RS16115 (WS70_14845) kdsD 3355317..3356300 (+) 984 WP_059471768.1 arabinose 5-phosphate isomerase KdsD -
  WS70_RS16120 (WS70_14850) - 3356300..3356839 (+) 540 WP_059471769.1 KdsC family phosphatase -
  WS70_RS16125 (WS70_14855) lptC 3356844..3357458 (+) 615 WP_059471770.1 LPS export ABC transporter periplasmic protein LptC -
  WS70_RS16130 (WS70_14860) lptA 3357477..3358142 (+) 666 WP_059471771.1 lipopolysaccharide transport periplasmic protein LptA -
  WS70_RS16135 (WS70_14865) lptB 3358139..3358915 (+) 777 WP_010113867.1 LPS export ABC transporter ATP-binding protein -
  WS70_RS16140 (WS70_14870) rpoN/rpoN1 3359083..3360591 (+) 1509 WP_059596767.1 RNA polymerase factor sigma-54 Machinery gene
  WS70_RS16150 (WS70_14875) hpf 3360744..3361103 (+) 360 WP_010101605.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WS70_RS16155 (WS70_14880) ptsN 3361339..3361848 (+) 510 WP_059471773.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WS70_RS16160 (WS70_14885) hprK 3361977..3362945 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  WS70_RS16165 (WS70_14890) rapZ 3363020..3363913 (+) 894 WP_059471774.1 RNase adapter RapZ -
  WS70_RS16170 (WS70_14895) - 3363940..3364572 (+) 633 WP_059596768.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55197.40 Da        Isoelectric Point: 4.8837

>NTDB_id=162419 WS70_RS16140 WP_059596767.1 3359083..3360591(+) (rpoN/rpoN1) [Burkholderia mayonis strain BDU6]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDDWIASPLRVAADGSVIAQTPASSAPEPMQ
GASNGTSATNERTERDEPAGVDEYDSYSADSGDASQWNLDDFGRSPSASDDDDLPPMQIHETATSLRDHLAAQLRVTQAS
PRDRALVMFLIESLDDDGYLTASLEEVLADLPPELEVDLDELNAALALLHSFDPAGVGARSPSECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTGQNWQAELNPEVV
PRLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=162419 WS70_RS16140 WP_059596767.1 3359083..3360591(+) (rpoN/rpoN1) [Burkholderia mayonis strain BDU6]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTCGCGCTCACCCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCGGTCGCGCAGAATCCGCTTCTCGAAAACGAAGACGACTGGA
TCGCGAGCCCGCTGCGCGTTGCGGCCGATGGCTCGGTGATCGCGCAGACGCCCGCATCGTCGGCGCCCGAGCCAATGCAG
GGCGCATCGAACGGCACGAGTGCGACGAATGAGCGCACCGAGCGCGACGAGCCTGCGGGCGTCGACGAATACGACAGCTA
TTCGGCCGACAGCGGCGATGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCTCCGCCTCGGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACGGCGACGTCGCTGCGCGACCATCTCGCCGCGCAACTGCGCGTCACGCAAGCGAGC
CCGCGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGAAGAAGT
CCTCGCCGATCTGCCGCCGGAGCTCGAGGTCGACCTCGACGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATC
CGGCAGGCGTCGGCGCGCGCTCCCCGTCCGAGTGCCTGAAGTTGCAATTGCTGCGGCTGGATCCGTCCCCGACGCGCGCG
CTCGCACTCGACATCGTGTCACAGCATCTCGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
ATCCGACGACGAGCTGCGCGATGCGCACGCGCTCATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAAGCCG
AAGCGGATTACGTGGTGCCCGACATCATCGTGAAGAAGACGGGTCAGAACTGGCAGGCGGAACTCAACCCGGAAGTCGTC
CCGCGGCTGCGGATCAACCATCTGTACGCGAACATCCTGCGCAATAGCCGGGGCGATCCGAGCGCGGGTTCGCTCAAGCA
GCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGATTCGAGACGATCCTGCGCGTCGCGCAAGCGATTG
TCGAACGTCAGAAGAATTTTTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTTTCGACAGACACCGGAGGTGCCGCGTCGTCGACGGCAATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGATAGTCGCATAGCCGAACTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTAAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

69.109

100

0.695

Multiple sequence alignment