Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WS66_RS15080 Genome accession   NZ_CP013384
Coordinates   530323..531828 (-) Length   501 a.a.
NCBI ID   WP_059598833.1    Uniprot ID   A0A3N8DJY3
Organism   Burkholderia sp. LA-2-3-30-S1-D2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525323..536828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WS66_RS15055 (WS66_14525) - 526360..526995 (-) 636 WP_059598835.1 LON peptidase substrate-binding domain-containing protein -
  WS66_RS15060 (WS66_14530) rapZ 527022..527930 (-) 909 WP_059526093.1 RNase adapter RapZ -
  WS66_RS15065 (WS66_14535) hprK 528007..528975 (-) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  WS66_RS15070 (WS66_14540) - 529095..529634 (-) 540 WP_059598834.1 PTS sugar transporter subunit IIA -
  WS66_RS15075 (WS66_14545) raiA 529804..530163 (-) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  WS66_RS15080 (WS66_14550) rpoN/rpoN1 530323..531828 (-) 1506 WP_059598833.1 RNA polymerase factor sigma-54 Machinery gene
  WS66_RS15085 (WS66_14555) lptB 531963..532739 (-) 777 WP_059598832.1 LPS export ABC transporter ATP-binding protein -
  WS66_RS15090 (WS66_14560) lptA 532736..533401 (-) 666 WP_059464489.1 lipopolysaccharide transport periplasmic protein LptA -
  WS66_RS15095 (WS66_14565) lptC 533422..534036 (-) 615 WP_059451451.1 LPS export ABC transporter periplasmic protein LptC -
  WS66_RS15100 (WS66_14570) - 534040..534576 (-) 537 WP_059598831.1 HAD family hydrolase -
  WS66_RS15105 (WS66_14575) kdsD 534576..535559 (-) 984 WP_059598830.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54791.90 Da        Isoelectric Point: 4.8740

>NTDB_id=162396 WS66_RS15080 WP_059598833.1 530323..531828(-) (rpoN/rpoN1) [Burkholderia sp. LA-2-3-30-S1-D2]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPSQNTESGA
ANGSSQSSDRAERDEPAAADEYDSYASGDAGDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162396 WS66_RS15080 WP_059598833.1 530323..531828(-) (rpoN/rpoN1) [Burkholderia sp. LA-2-3-30-S1-D2]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCACTGACACCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAGAACGATGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCTCCCAGCCAGAACACCGAGTCGGGCGCC
GCGAACGGCAGCAGCCAGTCGAGCGATCGCGCCGAGCGCGACGAACCGGCCGCCGCCGACGAATACGACAGCTACGCATC
GGGCGACGCCGGCGACGGCCCGCAGTGGAATCTCGACGAGTTCGGCCGCTCGAGCGGCGCATCGGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGTTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCTCCCTCGACGAAGTGCT
CGCCGACCTGCCGCCGGAACTGGAAATCGACTGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGTTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGAATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGACCCGTCGGCCGGATCGTTGAAACAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACTGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.972

100

0.697

Multiple sequence alignment