Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AQ610_RS16120 Genome accession   NZ_CP013380
Coordinates   3623483..3624991 (+) Length   502 a.a.
NCBI ID   WP_006024494.1    Uniprot ID   A0AAU8SQX0
Organism   Burkholderia humptydooensis strain MSMB43     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3618483..3629991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AQ610_RS16095 (AQ610_16075) kdsD 3619716..3620699 (+) 984 WP_006024499.1 arabinose 5-phosphate isomerase KdsD -
  AQ610_RS16100 (AQ610_16080) - 3620699..3621238 (+) 540 WP_009911188.1 HAD family hydrolase -
  AQ610_RS16105 (AQ610_16085) lptC 3621243..3621857 (+) 615 WP_006024497.1 LPS export ABC transporter periplasmic protein LptC -
  AQ610_RS16110 (AQ610_16090) lptA 3621876..3622541 (+) 666 WP_006024496.1 lipopolysaccharide transport periplasmic protein LptA -
  AQ610_RS16115 (AQ610_16095) lptB 3622538..3623314 (+) 777 WP_006024495.1 LPS export ABC transporter ATP-binding protein -
  AQ610_RS16120 (AQ610_16100) rpoN/rpoN1 3623483..3624991 (+) 1509 WP_006024494.1 RNA polymerase factor sigma-54 Machinery gene
  AQ610_RS16125 (AQ610_16105) hpf 3625141..3625500 (+) 360 WP_006024493.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AQ610_RS16130 (AQ610_16110) ptsN 3625733..3626242 (+) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AQ610_RS16135 (AQ610_16115) hprK 3626366..3627334 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  AQ610_RS16140 (AQ610_16120) rapZ 3627411..3628304 (+) 894 WP_006024491.1 RNase adapter RapZ -
  AQ610_RS16145 (AQ610_16125) - 3628331..3628963 (+) 633 WP_009911186.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55162.29 Da        Isoelectric Point: 4.8337

>NTDB_id=162391 AQ610_RS16120 WP_006024494.1 3623483..3624991(+) (rpoN/rpoN1) [Burkholderia humptydooensis strain MSMB43]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLESDDDWIASPLRVAADGSVIAQTPAPSAPEPMA
GASNGTTATNERTERDEAAGVDEYDSYSADSGDANQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLTASLEEVLADLPEELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTGQNWQAELNPEVV
PKLRINHLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=162391 AQ610_RS16120 WP_006024494.1 3623483..3624991(+) (rpoN/rpoN1) [Burkholderia humptydooensis strain MSMB43]
ATGAAAGCCAGTCTCCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAAAGCGACGACGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGCCGTCCGCGCCCGAGCCGATGGCG
GGCGCGTCGAACGGCACGACTGCGACGAATGAGCGCACCGAGCGCGACGAAGCCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACAGCGGCGACGCGAACCAGTGGAACCTCGACGATTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCCGAGGAGCTCGAAGTCGACGTCGACGAACTGAATGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CCGCGGGCGTCGGCGCGCGCTCCGCATCCGAATGCCTGAAGCTGCAACTGCTGCGCCTGGATCCGTCCCCGACGCGCACG
CTCGCCCTCGATATCGTGTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGAGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCATACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGACCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACCATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCACGGGATCGCTGAAGCA
GCAGCTTCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGATTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAACGTCAGAAGAATTTCTTTGCGCACGGCGAAATCGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAATCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACGCTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGCGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.762

100

0.697

Multiple sequence alignment