Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WS57_RS32770 Genome accession   NZ_CP013378
Coordinates   4428992..4430497 (+) Length   501 a.a.
NCBI ID   WP_040128221.1    Uniprot ID   A0A132F1F9
Organism   Burkholderia pseudomultivorans strain SUB-INT23-BP2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4423992..4435497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WS57_RS32745 (WS57_32685) kdsD 4425241..4426224 (+) 984 WP_040128218.1 arabinose 5-phosphate isomerase KdsD -
  WS57_RS32750 (WS57_32690) - 4426224..4426760 (+) 537 WP_060255080.1 KdsC family phosphatase -
  WS57_RS32755 (WS57_32695) lptC 4426764..4427378 (+) 615 WP_009693750.1 LPS export ABC transporter periplasmic protein LptC -
  WS57_RS32760 (WS57_32700) lptA 4427399..4428064 (+) 666 WP_009693751.1 lipopolysaccharide transport periplasmic protein LptA -
  WS57_RS32765 (WS57_32705) lptB 4428061..4428837 (+) 777 WP_009693752.1 LPS export ABC transporter ATP-binding protein -
  WS57_RS32770 (WS57_32710) rpoN/rpoN1 4428992..4430497 (+) 1506 WP_040128221.1 RNA polymerase factor sigma-54 Machinery gene
  WS57_RS32775 (WS57_32715) hpf 4430656..4431015 (+) 360 WP_009693755.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WS57_RS32780 (WS57_32720) - 4431213..4431725 (+) 513 WP_040129020.1 PTS sugar transporter subunit IIA -
  WS57_RS32785 (WS57_32725) hprK 4431854..4432822 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  WS57_RS32790 (WS57_32730) rapZ 4432890..4433798 (+) 909 WP_009693757.1 RNase adapter RapZ -
  WS57_RS32795 (WS57_32735) - 4433826..4434464 (+) 639 WP_009693758.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54803.93 Da        Isoelectric Point: 4.8683

>NTDB_id=162377 WS57_RS32770 WP_040128221.1 4428992..4430497(+) (rpoN/rpoN1) [Burkholderia pseudomultivorans strain SUB-INT23-BP2]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDDWIASPLRVAADGSLIAQTPPASNTEPAI
ANGNGQSTDRAERDEPAGADEYDAYASGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVIFLIESLDDDGYLTSTLDEILADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALDIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
KLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162377 WS57_RS32770 WP_040128221.1 4428992..4430497(+) (rpoN/rpoN1) [Burkholderia pseudomultivorans strain SUB-INT23-BP2]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTGGCGATGGCCGTCGCCCAGAACCCGCTGCTCGAAAACGACGACGACTGGA
TCGCCAGCCCGCTGCGCGTCGCGGCGGACGGATCGTTGATCGCGCAGACGCCGCCCGCGTCGAACACCGAGCCGGCCATC
GCGAACGGCAACGGCCAGTCGACCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGCCTACGCGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCGGACGACGACGACCTTC
CGCCGCTGCAGGTGCAGGAAGCCGCGACCTCGCTGCGCGACCACTTGTCCGCGCAACTGCGCGTCACGCAGGCCAGCCCG
CGCGACCGCGCGCTCGTGATCTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCTCGACCCTCGACGAGATCCT
CGCCGACCTGCCCCCCGAACTCGAAGTCGACTGCGACGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGTGCGCGCTCGGCGTCCGAGTGCCTGAAGCTGCAACTGCTGCGACTGGATCCGTCCCCGACCCGCACGCTC
GCGCTCGACATCGTGTCGCAGCATCTCGAGCTGCTGGCCGCGCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAGGTCGGCCAGGGCTGGCAGGCCGAGCTCAATCCCGAGGTCGTGCCG
AAGCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGCTTCGACACGATCCTGCGCGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCGCACGTTTCGACCGACACCGGAGGCGCCGCTTCGTCGACCGCCATCCGTGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCAAAATCGCCGCTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.577

100

0.693

Multiple sequence alignment