Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WS54_RS27565 Genome accession   NZ_CP013373
Coordinates   3012801..3014306 (+) Length   501 a.a.
NCBI ID   WP_059781474.1    Uniprot ID   A0AAN0ZB82
Organism   Burkholderia sp. NRF60-BP8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3007801..3019306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WS54_RS27540 (WS54_26025) kdsD 3009044..3010027 (+) 984 WP_059781478.1 arabinose 5-phosphate isomerase KdsD -
  WS54_RS27545 (WS54_26030) - 3010027..3010563 (+) 537 WP_059781476.1 HAD family hydrolase -
  WS54_RS27550 (WS54_26035) lptC 3010567..3011181 (+) 615 WP_034208413.1 LPS export ABC transporter periplasmic protein LptC -
  WS54_RS27555 (WS54_26040) lptA 3011202..3011867 (+) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  WS54_RS27560 (WS54_26045) lptB 3011864..3012640 (+) 777 WP_034208414.1 LPS export ABC transporter ATP-binding protein -
  WS54_RS27565 (WS54_26050) rpoN/rpoN1 3012801..3014306 (+) 1506 WP_059781474.1 RNA polymerase factor sigma-54 Machinery gene
  WS54_RS27570 (WS54_26055) raiA 3014465..3014824 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  WS54_RS27575 (WS54_26060) - 3015019..3015531 (+) 513 WP_027806888.1 PTS sugar transporter subunit IIA -
  WS54_RS27580 (WS54_26065) hprK 3015661..3016629 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  WS54_RS27585 (WS54_26070) rapZ 3016704..3017612 (+) 909 WP_006477783.1 RNase adapter RapZ -
  WS54_RS27590 (WS54_26075) - 3017640..3018275 (+) 636 WP_034208417.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54724.82 Da        Isoelectric Point: 4.8740

>NTDB_id=162347 WS54_RS27565 WP_059781474.1 3012801..3014306(+) (rpoN/rpoN1) [Burkholderia sp. NRF60-BP8]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLETDDEWIASPLRVAADGSLIAQTPPTQNTEPVA
TNGSGTSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSGGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162347 WS54_RS27565 WP_059781474.1 3012801..3014306(+) (rpoN/rpoN1) [Burkholderia sp. NRF60-BP8]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAACATCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAAACCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCACACAGAACACCGAACCCGTCGCG
ACGAACGGCAGCGGCACGTCCAGCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAATGGAACCTCGACGAATACGGCCGCTCGGGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAACTGGAAATCGACGGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCCTCCGAATGCCTGAAGCTGCAGTTGCTGCGTCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAGCTGCTCGCCGCCCGCGACTTCACACGGTTGCGCAAGCATCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAACTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAGCAGCA
GCTTCAGGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment