Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WI31_RS19435 Genome accession   NZ_CP013370
Coordinates   3245093..3246598 (+) Length   501 a.a.
NCBI ID   WP_059612836.1    Uniprot ID   A0A103CZS0
Organism   Burkholderia ubonensis strain RF23-BP41     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3240093..3251598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WI31_RS19410 (WI31_19395) kdsD 3241343..3242326 (+) 984 WP_059613267.1 arabinose 5-phosphate isomerase KdsD -
  WI31_RS19415 (WI31_19400) - 3242326..3242862 (+) 537 WP_059613269.1 KdsC family phosphatase -
  WI31_RS19420 (WI31_19405) lptC 3242866..3243480 (+) 615 WP_059613271.1 LPS export ABC transporter periplasmic protein LptC -
  WI31_RS19425 (WI31_19410) lptA 3243501..3244166 (+) 666 WP_059612832.1 lipopolysaccharide transport periplasmic protein LptA -
  WI31_RS19430 (WI31_19415) lptB 3244163..3244939 (+) 777 WP_010090668.1 LPS export ABC transporter ATP-binding protein -
  WI31_RS19435 (WI31_19420) rpoN/rpoN1 3245093..3246598 (+) 1506 WP_059612836.1 RNA polymerase factor sigma-54 Machinery gene
  WI31_RS19440 (WI31_19425) hpf 3246757..3247125 (+) 369 WP_010090666.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WI31_RS19445 (WI31_19430) - 3247316..3247828 (+) 513 WP_059612837.1 PTS sugar transporter subunit IIA -
  WI31_RS19450 (WI31_19435) hprK 3247965..3248933 (+) 969 WP_006398252.1 HPr(Ser) kinase/phosphatase -
  WI31_RS19455 (WI31_19440) rapZ 3249010..3249918 (+) 909 WP_059612840.1 RNase adapter RapZ -
  WI31_RS19460 (WI31_19445) - 3249945..3250583 (+) 639 WP_042586341.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54825.02 Da        Isoelectric Point: 4.8190

>NTDB_id=162333 WI31_RS19435 WP_059612836.1 3245093..3246598(+) (rpoN/rpoN1) [Burkholderia ubonensis strain RF23-BP41]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPASNSEPMA
ATNGAGQTGERTERDEPAAADDYDGYASGDNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQYLELLAARDFTRLRKHLKANDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162333 WI31_RS19435 WP_059612836.1 3245093..3246598(+) (rpoN/rpoN1) [Burkholderia ubonensis strain RF23-BP41]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAATCCGCTGCTCGAAAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCCGCGGACGGTTCGCTGATCGCGCAGACGCCGCCCGCGTCGAACTCCGAGCCGATGGCG
GCCACGAACGGCGCCGGCCAGACCGGCGAGCGCACCGAACGCGACGAGCCGGCCGCGGCCGACGACTACGACGGCTATGC
CTCCGGCGACAACGACGGCCCGCAATGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCGGATGACGACGACCTGC
CGCCGCTGCAGGTCCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGCTGCGCGTCACGCAGGCCGGGCCG
CGCGACCGCGCGCTTGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAACTCGAAGTCGATTGCGACGAACTGAACGCCGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAGTGCCTGAAGCTGCAGTTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAATATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAA
CGACGATGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAAG
CGGACTACGTCGTGCCCGACATCATCGTGAAGAAGGTCGGCCAGGGCTGGCAGGCGGAGCTGAATCCCGAAGTCGTGCCG
CGCCTGCGGATCAACAATCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCCGCGGGTTCGCTCAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGACACGATCCTGCGAGTTGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCACACGGCGAAATCGCTATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTGATCAAGCAACTGATAG
GAGCCGAAGACACCAAATCGCCTCTTTCGGACAGCCGAATTGCCGAACTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A103CZS0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691

Multiple sequence alignment