Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WI26_RS13470 Genome accession   NZ_CP013362
Coordinates   2925038..2926543 (+) Length   501 a.a.
NCBI ID   WP_059511932.1    Uniprot ID   A0AAW3PAX3
Organism   Burkholderia diffusa strain RF2-non-BP9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2920038..2931543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WI26_RS13445 (WI26_13435) kdsD 2921279..2922262 (+) 984 WP_069226166.1 arabinose 5-phosphate isomerase KdsD -
  WI26_RS13450 (WI26_13440) - 2922262..2922798 (+) 537 WP_069226167.1 KdsC family phosphatase -
  WI26_RS13455 (WI26_13445) lptC 2922802..2923416 (+) 615 WP_059464490.1 LPS export ABC transporter periplasmic protein LptC -
  WI26_RS13460 (WI26_13450) lptA 2923437..2924102 (+) 666 WP_059464489.1 lipopolysaccharide transport periplasmic protein LptA -
  WI26_RS13465 (WI26_13455) lptB 2924099..2924875 (+) 777 WP_044847462.1 LPS export ABC transporter ATP-binding protein -
  WI26_RS13470 (WI26_13460) rpoN/rpoN1 2925038..2926543 (+) 1506 WP_059511932.1 RNA polymerase factor sigma-54 Machinery gene
  WI26_RS13475 (WI26_13465) hpf 2926703..2927062 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WI26_RS13480 (WI26_13470) - 2927262..2927774 (+) 513 WP_059451610.1 PTS sugar transporter subunit IIA -
  WI26_RS13485 (WI26_13475) hprK 2927894..2928862 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  WI26_RS13490 (WI26_13480) rapZ 2928938..2929846 (+) 909 WP_059541313.1 RNase adapter RapZ -
  WI26_RS13495 (WI26_13485) - 2929873..2930508 (+) 636 WP_059541310.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54697.84 Da        Isoelectric Point: 4.8740

>NTDB_id=162303 WI26_RS13470 WP_059511932.1 2925038..2926543(+) (rpoN/rpoN1) [Burkholderia diffusa strain RF2-non-BP9]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPGQNTEPGA
ANGSSQSGDRAERDEPAAADEYDSYASGDAGDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162303 WI26_RS13470 WP_059511932.1 2925038..2926543(+) (rpoN/rpoN1) [Burkholderia diffusa strain RF2-non-BP9]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCACTGACACCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAGAACGATGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCTCCCGGCCAGAACACCGAGCCGGGCGCC
GCGAACGGCAGCAGCCAGTCGGGCGATCGCGCCGAGCGCGACGAGCCGGCCGCCGCCGACGAATACGACAGCTACGCATC
GGGCGACGCCGGCGACGGCCCGCAATGGAATCTCGACGAGTTCGGCCGCTCGAGCGGCGCATCGGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCGGCCCG
CGAGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCTCCCTCGACGAAGTCCT
CGCCGATCTGCCGCCCGAGCTGGAAGTCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CGGGGGTCGGGGCCCGCTCGGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCCACTCGCACGCTC
GCGCTGGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGTTGCGCAAGCATCTGAAGGCGAG
CGACGACGCGCTGCGCGATGCGCATGCGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAACAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACTGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

69.17

100

0.699

Multiple sequence alignment