Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   WI26_RS09685 Genome accession   NZ_CP013362
Coordinates   2103351..2104727 (-) Length   458 a.a.
NCBI ID   WP_059464241.1    Uniprot ID   -
Organism   Burkholderia diffusa strain RF2-non-BP9     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2098351..2109727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WI26_RS09665 (WI26_09650) cls 2098635..2100074 (+) 1440 WP_069225835.1 cardiolipin synthase -
  WI26_RS09670 (WI26_09655) - 2100195..2100674 (-) 480 WP_059595124.1 glutathione peroxidase -
  WI26_RS09675 (WI26_09660) - 2100811..2102742 (+) 1932 WP_069225836.1 ATP-binding cassette domain-containing protein -
  WI26_RS09680 (WI26_09665) - 2102935..2103201 (-) 267 WP_059464242.1 DUF2866 domain-containing protein -
  WI26_RS09685 (WI26_09670) radA 2103351..2104727 (-) 1377 WP_059464241.1 DNA repair protein RadA Machinery gene
  WI26_RS09690 (WI26_09675) alr 2104739..2105809 (-) 1071 WP_069225837.1 alanine racemase -
  WI26_RS09695 (WI26_09680) lplT 2106028..2107335 (+) 1308 WP_059464239.1 lysophospholipid transporter LplT -
  WI26_RS09700 (WI26_09685) thiD 2107394..2108203 (+) 810 WP_059464238.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  WI26_RS09705 (WI26_09690) - 2108222..2109181 (-) 960 WP_069225838.1 DUF1853 family protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48835.31 Da        Isoelectric Point: 6.9501

>NTDB_id=162299 WI26_RS09685 WP_059464241.1 2103351..2104727(-) (radA) [Burkholderia diffusa strain RF2-non-BP9]
MAKQKTVYVCSECGGQTPKWQGQCPSCQAWNTLVESVAESPTAHRFQSLAKRAPVQRLVDIEAADVPRFSTGIGEFDRVL
GGGLVPGGVVLIGGDPGIGKSTLLLQSLADIASERRALYISGEESAAQIALRAQRLALLDGGAPAAELKLLAEIQLEKIQ
AAIDAERPDVAVIDSIQTVYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQVVPGSCVLVTQEGTRPLLVEIQALVDTAHV
PNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKALPKGLIVFGEVGLA
GEIRPSPRGQERLREAAKLGFTAALIPKANAPKQPIEGLNVMAVERIEQAIDRVRDLE

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=162299 WI26_RS09685 WP_059464241.1 2103351..2104727(-) (radA) [Burkholderia diffusa strain RF2-non-BP9]
GTGGCCAAACAGAAAACCGTCTACGTCTGCAGCGAGTGCGGCGGACAAACCCCGAAGTGGCAGGGGCAATGCCCGTCGTG
CCAGGCCTGGAACACGCTCGTCGAATCGGTCGCCGAGTCGCCTACCGCCCATCGCTTCCAGTCGCTCGCGAAGCGCGCGC
CCGTGCAGCGGCTCGTCGACATCGAGGCGGCCGACGTGCCGCGCTTCTCCACCGGCATCGGCGAATTCGACCGTGTGCTG
GGCGGCGGGCTGGTGCCGGGCGGCGTCGTGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCGACGCTGTTGCTGCAGTC
GCTCGCGGACATCGCCAGCGAGCGTCGCGCACTCTATATCAGCGGCGAGGAATCGGCCGCGCAGATCGCGTTGCGCGCGC
AACGGCTCGCGCTGCTCGACGGCGGCGCGCCGGCGGCCGAGTTGAAGCTGCTCGCGGAGATCCAGCTCGAGAAGATCCAG
GCCGCGATCGACGCTGAACGGCCGGACGTCGCCGTCATCGACTCGATCCAGACCGTCTATTCCGAAGCACTGACGTCGGC
GCCCGGTTCGGTCGCCCAGGTGCGCGAGTGCGCGGCGCAGCTCACGCGCATCGCGAAGCAGTCCGGCACGGCGATCATCA
TGGTCGGGCACGTGACGAAGGAGGGCAACCTCGCCGGCCCGCGCGTGCTCGAGCACATCGTCGACACGGTGCTGTACTTC
GAAGGCGACACCCATTCGTCGTTCCGGCTCGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAACTCGGCGTGTT
CGCGATGACCGAGCGCGGGCTGCGCGGTGTCGCGAACCCGTCCGCGCTGTTCCTGTCGCAGCACGAGCAGGTCGTGCCGG
GTTCCTGCGTGCTCGTCACGCAGGAAGGCACGCGCCCGCTGCTCGTCGAAATCCAGGCGCTCGTCGATACGGCCCACGTG
CCGAACCCGCGCCGGCTCGCGGTTGGCCTCGAACAGAACCGGCTCGCGATGCTGCTCGCGGTGCTGCATCGGCACGCGGG
CATCGCGTGTTTCGATCAGGACGTGTTCCTCAACGCGGTGGGTGGCGTGAAGATCACCGAGCCGGCCGCCGACCTCGCGG
TGCTGCTCGCGATTCATTCGTCGATGCGCAACAAGGCGCTGCCGAAGGGCCTGATCGTGTTCGGCGAAGTGGGGCTGGCG
GGCGAGATCCGGCCGTCCCCGCGTGGCCAGGAACGGCTGCGCGAGGCGGCGAAGCTTGGTTTCACCGCGGCGCTGATCCC
GAAGGCGAACGCACCGAAACAGCCGATCGAAGGGCTGAACGTGATGGCCGTCGAGCGGATCGAGCAGGCGATCGACCGCG
TACGCGATCTCGAATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.458

100

0.498
  radA Streptococcus mitis SK321

47.162

100

0.472
  radA Streptococcus pneumoniae Rx1

46.855

100

0.472
  radA Streptococcus pneumoniae D39

46.855

100

0.472
  radA Streptococcus pneumoniae R6

46.855

100

0.472
  radA Streptococcus pneumoniae TIGR4

46.855

100

0.472
  radA Streptococcus mitis NCTC 12261

46.827

99.782

0.467

Multiple sequence alignment