Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WI24_RS15070 Genome accession   NZ_CP013360
Coordinates   3312886..3314394 (+) Length   502 a.a.
NCBI ID   WP_009902786.1    Uniprot ID   A0AAW9CXE8
Organism   Burkholderia thailandensis strain 2003015869     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3307886..3319394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WI24_RS15045 (WI24_15035) kdsD 3309117..3310100 (+) 984 WP_009893041.1 arabinose 5-phosphate isomerase KdsD -
  WI24_RS15050 (WI24_15040) - 3310100..3310639 (+) 540 WP_009902788.1 KdsC family phosphatase -
  WI24_RS15055 (WI24_15045) lptC 3310644..3311258 (+) 615 WP_009893044.1 LPS export ABC transporter periplasmic protein LptC -
  WI24_RS15060 (WI24_15050) lptA 3311277..3311942 (+) 666 WP_009893045.1 lipopolysaccharide transport periplasmic protein LptA -
  WI24_RS15065 (WI24_15055) lptB 3311939..3312715 (+) 777 WP_009902787.1 LPS export ABC transporter ATP-binding protein -
  WI24_RS15070 (WI24_15060) rpoN/rpoN1 3312886..3314394 (+) 1509 WP_009902786.1 RNA polymerase factor sigma-54 Machinery gene
  WI24_RS15075 (WI24_15065) hpf 3314544..3314903 (+) 360 WP_009893049.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WI24_RS15080 (WI24_15070) - 3315136..3315645 (+) 510 WP_161654448.1 PTS sugar transporter subunit IIA -
  WI24_RS15085 (WI24_15075) hprK 3315774..3316742 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  WI24_RS15090 (WI24_15080) rapZ 3316819..3317712 (+) 894 WP_009893058.1 RNase adapter RapZ -
  WI24_RS15095 (WI24_15085) - 3317739..3318371 (+) 633 WP_009893059.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55011.08 Da        Isoelectric Point: 4.8782

>NTDB_id=162289 WI24_RS15070 WP_009902786.1 3312886..3314394(+) (rpoN/rpoN1) [Burkholderia thailandensis strain 2003015869]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSSPEPMS
GASNGTTATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLDEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQSWQAELNPEVV
PKLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=162289 WI24_RS15070 WP_009902786.1 3312886..3314394(+) (rpoN/rpoN1) [Burkholderia thailandensis strain 2003015869]
ATGAAAGCCAGTCTCCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAATCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCAACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGACGACTGGA
TCGCGAGCCCCCTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCTCGCCCGAGCCGATGTCC
GGCGCGTCGAACGGCACGACTGCGACGAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAATCTCGACGACTTCGGCCGCTCCCCCGCCGCATCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACCTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGACGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAAGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGGTCCGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGGCTGGATCCGTCCCCGACGCGCACG
CTCGCGCTCGATATCGTGTCGCAGCATCTCGAGCTGCTTGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGAGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTTCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAGCTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGTATCAACCATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
GCAGCTTCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGGTTCGAGACGATCCTGCGGGTCGCGCAGGCGATTG
TCGAACGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACTCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.713

100

0.691

Multiple sequence alignment