Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WI23_RS17660 Genome accession   NZ_CP013358
Coordinates   3653986..3655494 (+) Length   502 a.a.
NCBI ID   WP_010113865.1    Uniprot ID   -
Organism   Burkholderia oklahomensis C6786     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3648986..3660494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WI23_RS17635 (WI23_16050) kdsD 3650220..3651203 (+) 984 WP_010113875.1 arabinose 5-phosphate isomerase KdsD -
  WI23_RS17640 (WI23_16055) - 3651203..3651742 (+) 540 WP_010113872.1 KdsC family phosphatase -
  WI23_RS17645 (WI23_16060) lptC 3651747..3652361 (+) 615 WP_010101611.1 LPS export ABC transporter periplasmic protein LptC -
  WI23_RS17650 (WI23_16065) lptA 3652380..3653045 (+) 666 WP_010113870.1 lipopolysaccharide transport periplasmic protein LptA -
  WI23_RS17655 (WI23_16070) lptB 3653042..3653818 (+) 777 WP_010113867.1 LPS export ABC transporter ATP-binding protein -
  WI23_RS17660 (WI23_16075) rpoN/rpoN1 3653986..3655494 (+) 1509 WP_010113865.1 RNA polymerase factor sigma-54 Machinery gene
  WI23_RS17665 (WI23_16080) hpf 3655647..3656006 (+) 360 WP_010101605.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WI23_RS17670 (WI23_16085) ptsN 3656242..3656751 (+) 510 WP_025989646.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WI23_RS17675 (WI23_16090) hprK 3656880..3657848 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  WI23_RS17680 (WI23_16095) rapZ 3657923..3658816 (+) 894 WP_010101593.1 RNase adapter RapZ -
  WI23_RS17685 (WI23_16100) - 3658843..3659475 (+) 633 WP_010101591.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55194.38 Da        Isoelectric Point: 4.9394

>NTDB_id=162275 WI23_RS17660 WP_010113865.1 3653986..3655494(+) (rpoN/rpoN1) [Burkholderia oklahomensis C6786]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDDWIASPLRVAADGSVIAQAPASSAPEPMQ
GASNNTSATNERTERDEPAGVDEYDSYSADSGDASQWNLDDFGRSPSASDDDDLPPMQIHETTTSLRDHLAAQLRVTQAS
PRDRALVLFLIESLDDDGYLTASLEEVLADLPAELEIDLDELNAALALLHSFDPAGVGARSPSECLKLQLLRLNPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQSWQAELNPEVV
PRLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQRNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=162275 WI23_RS17660 WP_010113865.1 3653986..3655494(+) (rpoN/rpoN1) [Burkholderia oklahomensis C6786]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTCGCGCTCACCCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCGGTCGCGCAGAATCCGCTTCTCGAAAACGAAGACGACTGGA
TCGCGAGCCCGCTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGGCGCCCGCATCGTCTGCGCCCGAGCCGATGCAA
GGCGCATCGAACAACACGAGTGCGACGAATGAGCGCACCGAGCGCGACGAGCCTGCAGGCGTCGACGAATACGACAGCTA
TTCGGCCGACAGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCTCCGCTTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACGACGACGTCGCTGCGCGACCATCTCGCCGCGCAACTGCGCGTCACGCAAGCGAGC
CCGCGCGATCGCGCGCTGGTGCTGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGAAGAAGT
GCTCGCCGATCTGCCGGCGGAACTCGAGATCGACCTCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATC
CTGCAGGCGTCGGCGCGCGCTCCCCATCCGAGTGCCTGAAGCTGCAGTTGCTGCGGCTGAATCCGTCCCCGACGCGCGCG
CTCGCGCTCGACATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
ATCCGACGACGAGCTGCGCGACGCGCACGCGCTCATCCGCTCGCTCGAGCCTTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCGGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCGGGGCAGAGCTGGCAGGCGGAACTGAATCCGGAAGTCGTG
CCGCGGCTGCGGATCAACCATCTGTACGCGAACATCCTGCGCAACAGCCGAGGCGATCCGAGCGCGGGTTCGCTCAAGCA
GCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGATTCGAGACGATCCTGCGCGTCGCGCAAGCGATTG
TCGAACGTCAGAGGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACGCTTGAATTTAAGTA
CTTCTTCGGATCGCACGTTTCGACAGACACCGGAGGTGCCGCGTCGTCGACGGCAATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGATAGTCGCATAGCCGAACTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.254

100

0.685

Multiple sequence alignment