Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WG70_RS32080 Genome accession   NZ_CP013357
Coordinates   3689159..3690667 (+) Length   502 a.a.
NCBI ID   WP_010101606.1    Uniprot ID   A0AAI8B3F6
Organism   Burkholderia oklahomensis EO147     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3684159..3695667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WG70_RS32055 (WG70_29340) kdsD 3685393..3686376 (+) 984 WP_010101613.1 arabinose 5-phosphate isomerase KdsD -
  WG70_RS32060 (WG70_29345) - 3686376..3686915 (+) 540 WP_010101612.1 KdsC family phosphatase -
  WG70_RS32065 (WG70_29350) lptC 3686920..3687534 (+) 615 WP_010101611.1 LPS export ABC transporter periplasmic protein LptC -
  WG70_RS32070 (WG70_29355) lptA 3687553..3688218 (+) 666 WP_010101609.1 lipopolysaccharide transport periplasmic protein LptA -
  WG70_RS32075 (WG70_29360) lptB 3688215..3688991 (+) 777 WP_010101607.1 LPS export ABC transporter ATP-binding protein -
  WG70_RS32080 (WG70_29365) rpoN/rpoN1 3689159..3690667 (+) 1509 WP_010101606.1 RNA polymerase factor sigma-54 Machinery gene
  WG70_RS32090 (WG70_29370) hpf 3690820..3691179 (+) 360 WP_010101605.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WG70_RS32095 (WG70_29375) ptsN 3691415..3691924 (+) 510 WP_025989646.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WG70_RS32100 (WG70_29380) hprK 3692053..3693021 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  WG70_RS32105 (WG70_29385) rapZ 3693096..3693989 (+) 894 WP_010101593.1 RNase adapter RapZ -
  WG70_RS32110 (WG70_29390) - 3694016..3694648 (+) 633 WP_010101591.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55199.40 Da        Isoelectric Point: 4.9411

>NTDB_id=162260 WG70_RS32080 WP_010101606.1 3689159..3690667(+) (rpoN/rpoN1) [Burkholderia oklahomensis EO147]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDDWIASPLRVAADGSVIAQAPASSAPEPMQ
GASNNTSATNERTERDEPAGVDEYDSYSADSGDASQWNLDDFGRSPSASDDDDLPPMQIHETTTSLRDHLAAQLRVTQAS
PRDRALVLFLIESLDDDGYLTASLEEVLADLPAELEVDLDELNAALALLHSFDPAGVGARSPSECLKLQLLRLNPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKASDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQSWQAELNPEVV
PRLRINRLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQRNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=162260 WG70_RS32080 WP_010101606.1 3689159..3690667(+) (rpoN/rpoN1) [Burkholderia oklahomensis EO147]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTCGCGCTCACACCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCGGTCGCGCAGAATCCGCTTCTCGAAAACGAAGACGACTGGA
TCGCGAGCCCGCTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGGCGCCCGCATCGTCTGCGCCCGAGCCGATGCAA
GGCGCATCGAACAACACGAGTGCGACGAATGAGCGCACCGAGCGCGACGAGCCTGCAGGCGTCGACGAATACGACAGCTA
TTCGGCCGACAGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCTCCGCTTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACGACGACGTCGCTGCGCGACCATCTCGCCGCGCAACTGCGCGTCACGCAAGCGAGC
CCGCGCGATCGCGCGCTGGTGCTGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGAAGAAGT
GCTCGCCGATCTGCCGGCGGAACTCGAGGTCGACCTCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATC
CTGCAGGCGTCGGCGCGCGCTCCCCATCCGAGTGCCTGAAGCTGCAGTTGCTGCGGCTGAATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GTCCGACGACGAGCTGCGCGACGCGCACGCGCTCATCCGCTCGCTCGAGCCTTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCGGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCGGGGCAGAGCTGGCAGGCGGAACTCAATCCGGAAGTCGTG
CCGCGGCTGCGGATCAACCGTCTGTACGCGAACATCCTGCGCAACAGCCGAGGCGATCCGAGCGCGGGTTCGCTCAAGCA
GCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGGTTCGAGACGATCTTGCGCGTCGCGCAAGCGATTG
TCGAACGTCAGAGGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACGCTTGAATTTAAGTA
CTTCTTCGGATCGCACGTTTCGACAGACACCGGAGGTGCCGCGTCGTCGACGGCAATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGATAGTCGCATAGCCGAACTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.452

100

0.687

Multiple sequence alignment