Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   NPD1_RS06790 Genome accession   NZ_CP013296
Coordinates   1118949..1120307 (-) Length   452 a.a.
NCBI ID   WP_003357575.1    Uniprot ID   A5I7N5
Organism   Clostridium botulinum strain CDC_54064     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1113949..1125307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPD1_RS06770 (NPD1_1106) ispD 1115243..1115932 (-) 690 WP_003357598.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  NPD1_RS06775 (NPD1_1107) - 1115934..1117034 (-) 1101 WP_003357294.1 PIN/TRAM domain-containing protein -
  NPD1_RS06780 (NPD1_1109) - 1117439..1117837 (+) 399 WP_003357596.1 hypothetical protein -
  NPD1_RS06785 (NPD1_1110) disA 1117869..1118930 (-) 1062 WP_003357541.1 DNA integrity scanning diadenylate cyclase DisA -
  NPD1_RS06790 (NPD1_1111) radA 1118949..1120307 (-) 1359 WP_003357575.1 DNA repair protein RadA Machinery gene
  NPD1_RS06795 (NPD1_1112) - 1120427..1122862 (-) 2436 WP_012705182.1 ATP-dependent Clp protease ATP-binding subunit -
  NPD1_RS06800 (NPD1_1113) - 1122864..1123907 (-) 1044 WP_003357574.1 protein arginine kinase -
  NPD1_RS06805 (NPD1_1114) - 1123912..1124439 (-) 528 WP_012704188.1 UvrB/UvrC motif-containing protein -
  NPD1_RS06810 (NPD1_1115) - 1124462..1124926 (-) 465 WP_003357349.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49749.75 Da        Isoelectric Point: 8.1297

>NTDB_id=161588 NPD1_RS06790 WP_003357575.1 1118949..1120307(-) (radA) [Clostridium botulinum strain CDC_54064]
MAKNKIVYICQQCGYESIKWLGKCPGCNSWNSMVEEEKQSTNKTIKNINIKSEPKIISTIKSSEYERLDTGINELNRVLG
GGIVKGSLTLISGSPGIGKSTILLQAANNIATKYGKILYVSGEESEEQIKMRADRLKVISKDIYILSETNVDIIKEHITT
INPKFVIIDSIQTLFKSELSSAPGTVSQVRQCSNDIMLISKSNNIPFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTVKNRFGTTSEIGVFEMAGEGLLEISNPSAVFLEETEFQREGSVIIGIMEGTRPILIEIQALVSETKAYMPRR
TAVGVDTARLNLILAVLEKKLNIPFYNCDVYVNVVGGLDLTGTFADLGLALALLSSAKSKDIKLPKVLVVGEIGLTGEVR
PVSFCDRIVNEGIKMGFTNIIIPDRNKDKISVKNNVNLIGISSLREGINKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=161588 NPD1_RS06790 WP_003357575.1 1118949..1120307(-) (radA) [Clostridium botulinum strain CDC_54064]
ATGGCTAAAAATAAAATTGTTTATATATGTCAACAATGTGGATATGAATCGATAAAATGGTTAGGAAAATGTCCAGGATG
CAATTCATGGAATAGTATGGTAGAGGAAGAAAAGCAATCAACTAATAAGACTATAAAAAATATAAATATTAAATCAGAGC
CTAAAATAATATCTACAATAAAATCAAGTGAATATGAGAGATTAGATACTGGAATAAATGAACTGAATAGAGTCTTAGGC
GGTGGTATAGTTAAGGGCTCTTTAACTCTTATATCTGGGTCTCCTGGCATAGGAAAGTCTACAATACTTTTACAAGCTGC
TAATAATATTGCTACTAAGTATGGTAAGATTTTATATGTGTCTGGAGAAGAATCAGAAGAGCAGATAAAAATGAGAGCAG
ATAGGCTTAAGGTGATATCAAAAGATATATACATACTTTCAGAGACAAATGTGGATATAATAAAAGAGCATATTACTACA
ATTAATCCTAAATTTGTTATAATAGATTCTATACAGACCCTTTTTAAAAGTGAACTATCTTCAGCTCCAGGTACTGTGTC
TCAAGTTAGGCAATGTTCTAACGATATTATGCTTATAAGTAAAAGTAATAACATACCTTTTTTTATAGTAGCGCATGTTA
CAAAACAAGGAGAATTAGCAGGACCTAGAGTTTTAGAACATATGGTAGATACGGTTTTATCTTTTGAAGGAGAAAGAACC
CAGGAATTTAGGATTTTAAGAACAGTAAAAAATCGTTTTGGAACTACAAGTGAAATTGGAGTTTTTGAAATGGCAGGAGA
AGGGTTATTAGAAATAAGCAATCCCTCTGCTGTATTTTTAGAAGAAACTGAATTTCAAAGGGAAGGTTCTGTAATAATAG
GTATAATGGAAGGGACTAGACCAATACTTATAGAAATACAAGCATTAGTAAGTGAAACGAAAGCTTATATGCCAAGAAGG
ACGGCAGTGGGAGTAGACACAGCTAGATTGAATCTAATATTAGCTGTGTTAGAAAAAAAATTAAATATACCTTTCTATAA
TTGTGATGTATATGTTAATGTAGTAGGAGGATTAGATTTAACAGGAACTTTTGCTGATTTGGGATTAGCATTAGCTTTAT
TATCTAGTGCTAAATCAAAAGATATAAAGTTACCTAAAGTTTTAGTGGTTGGAGAAATAGGACTTACAGGAGAGGTTCGC
CCCGTAAGTTTTTGTGATAGAATAGTAAATGAAGGTATAAAAATGGGATTTACCAATATAATTATACCTGATAGAAATAA
AGACAAAATTTCTGTTAAAAATAATGTTAACCTAATTGGTATATCTTCTTTAAGAGAAGGTATTAATAAAGTATTTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-217)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A5I7N5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.11

100

0.502
  radA Streptococcus pneumoniae Rx1

48.899

100

0.491
  radA Streptococcus pneumoniae D39

48.899

100

0.491
  radA Streptococcus pneumoniae R6

48.899

100

0.491
  radA Streptococcus pneumoniae TIGR4

48.899

100

0.491
  radA Streptococcus mitis NCTC 12261

48.899

100

0.491
  radA Streptococcus mitis SK321

48.899

100

0.491

Multiple sequence alignment