Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ATN06_RS21635 Genome accession   NZ_CP013274
Coordinates   4147250..4148293 (-) Length   347 a.a.
NCBI ID   WP_060632267.1    Uniprot ID   A0A243GEI2
Organism   Bacillus thuringiensis strain CTC     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4142250..4153293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATN06_RS21590 (ATN06_21360) - 4142681..4142881 (-) 201 WP_000106080.1 YqzE family protein -
  ATN06_RS21595 (ATN06_21365) - 4142920..4143417 (-) 498 WP_060632259.1 shikimate kinase -
  ATN06_RS21600 (ATN06_21370) - 4143536..4144186 (-) 651 WP_060632260.1 prolyl hydroxylase family protein -
  ATN06_RS21605 (ATN06_21375) comGG 4144360..4144731 (-) 372 WP_060632261.1 competence type IV pilus minor pilin ComGG -
  ATN06_RS21610 (ATN06_21380) comGF 4144728..4145198 (-) 471 WP_060632262.1 competence type IV pilus minor pilin ComGF -
  ATN06_RS21615 (ATN06_21385) comGE 4145168..4145470 (-) 303 WP_060632263.1 competence type IV pilus minor pilin ComGE -
  ATN06_RS21620 (ATN06_21390) comGD 4145463..4145918 (-) 456 WP_060632264.1 comG operon protein ComGD -
  ATN06_RS21625 (ATN06_21395) comGC 4145915..4146214 (-) 300 WP_060632265.1 comG operon protein ComGC -
  ATN06_RS21630 (ATN06_21400) comGB 4146226..4147257 (-) 1032 WP_060632266.1 competence type IV pilus assembly protein ComGB -
  ATN06_RS21635 (ATN06_21405) comGA 4147250..4148293 (-) 1044 WP_060632267.1 competence protein ComGA Machinery gene
  ATN06_RS21640 (ATN06_21410) - 4148499..4149194 (+) 696 WP_060632268.1 helix-turn-helix transcriptional regulator -
  ATN06_RS21645 (ATN06_21415) - 4149320..4149562 (+) 243 WP_000440717.1 DUF2626 domain-containing protein -
  ATN06_RS21650 (ATN06_21420) - 4149669..4151063 (+) 1395 WP_060632269.1 L-cystine transporter -
  ATN06_RS21655 (ATN06_21425) - 4151280..4151495 (-) 216 WP_001008328.1 DUF3912 family protein -
  ATN06_RS21660 (ATN06_21430) - 4151748..4152059 (+) 312 WP_060632270.1 hypothetical protein -
  ATN06_RS21665 (ATN06_21435) - 4152096..4152584 (-) 489 WP_060632271.1 hypothetical protein -
  ATN06_RS21670 (ATN06_21440) - 4152666..4153040 (-) 375 WP_060632272.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39343.91 Da        Isoelectric Point: 9.0719

>NTDB_id=161291 ATN06_RS21635 WP_060632267.1 4147250..4148293(-) (comGA) [Bacillus thuringiensis strain CTC]
MNGIESFANTILKEACRVQASDLHIVPRQKDVAIQLRIGKDLMMKHCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDLLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAILRFMDFGVTRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLEASIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=161291 ATN06_RS21635 WP_060632267.1 4147250..4148293(-) (comGA) [Bacillus thuringiensis strain CTC]
ATGAATGGAATTGAAAGTTTTGCGAATACGATTTTGAAAGAAGCGTGTAGGGTACAAGCGTCGGACTTACATATCGTGCC
GAGGCAGAAGGACGTGGCAATTCAACTACGTATAGGAAAAGATTTAATGATGAAACATTGTATTGAAAAGGAATTTGGAG
AAAAACTCGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGAGAGAGGCGAAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTACGCCTTTCCACGCTTCCAACTGTATATCAAGAAAGTCTCGTTATTCGTCT
CCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCAAAAAAATTACTCTCTTTTTTAA
GGTATTCACATGGGTTACTTGTATTTACTGGACCGACTGGTTCTGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACGCGCCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCGGGACTAAAGGCTATTTTACGCCATGATCCAGATATTATTTTAGTTG
GTGAAATCCGTGATGAAGAAACAGCGAAAATCGCAATAAGAGCAAGTTTGACTGGACATTTAGTAATGACGACACTGCAT
ACGAATGATGCGAAAGGGGCGATACTCAGGTTCATGGATTTTGGCGTAACGAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGACAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAATGGAGAATGT
GTCACATACAAGCATGAAACATTAGAAGCTTCGATACGAAAAGGATACGCGTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243GEI2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.694

100

0.363

Multiple sequence alignment