Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ATN06_RS20370 Genome accession   NZ_CP013274
Coordinates   3940485..3940919 (-) Length   144 a.a.
NCBI ID   WP_060632098.1    Uniprot ID   A0A6I7XKP6
Organism   Bacillus thuringiensis strain CTC     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3935485..3945919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATN06_RS20350 (ATN06_20120) - 3935891..3936472 (+) 582 WP_060632094.1 PadR family transcriptional regulator -
  ATN06_RS20355 (ATN06_20125) - 3936654..3937085 (-) 432 WP_060632095.1 NUDIX hydrolase -
  ATN06_RS20360 (ATN06_20130) metE 3937734..3940022 (+) 2289 WP_060632096.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  ATN06_RS20365 (ATN06_20135) comJ 3940059..3940448 (-) 390 WP_060632097.1 competence protein ComJ -
  ATN06_RS20370 (ATN06_20140) nucA/comI 3940485..3940919 (-) 435 WP_060632098.1 DNA-entry nuclease Machinery gene
  ATN06_RS20375 (ATN06_20145) - 3941156..3941905 (+) 750 WP_060632099.1 DUF3967 domain-containing protein -
  ATN06_RS20380 (ATN06_20150) kinB 3941944..3943218 (-) 1275 WP_060632100.1 sporulation sensor histidine kinase KinB -
  ATN06_RS20385 (ATN06_20155) - 3943528..3943728 (+) 201 WP_000929285.1 hypothetical protein -
  ATN06_RS20390 (ATN06_20160) - 3943852..3945015 (+) 1164 WP_060632101.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.31 Da        Isoelectric Point: 7.8613

>NTDB_id=161288 ATN06_RS20370 WP_060632098.1 3940485..3940919(-) (nucA/comI) [Bacillus thuringiensis strain CTC]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTQKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=161288 ATN06_RS20370 WP_060632098.1 3940485..3940919(-) (nucA/comI) [Bacillus thuringiensis strain CTC]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACCCAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACCGGGA
AACATATTACGGATGCGATAAAAGAAGGACATTCAGAAGTTTGTACAATTGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGGAATAAGCTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I7XKP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment