Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC22_RS14735 Genome accession   NZ_CP013248
Coordinates   3023082..3024098 (-) Length   338 a.a.
NCBI ID   WP_071652433.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_022     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3018082..3029098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC22_RS14710 (FORC22_2782) aroK 3018942..3019460 (-) 519 WP_005381319.1 shikimate kinase AroK -
  FORC22_RS14715 (FORC22_2783) pilQ 3019649..3021397 (-) 1749 WP_071652432.1 type IV pilus secretin PilQ Machinery gene
  FORC22_RS14720 (FORC22_2784) pilP 3021429..3021944 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  FORC22_RS14725 (FORC22_2785) pilO 3021934..3022527 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC22_RS14730 (FORC22_2786) pilN 3022520..3023098 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  FORC22_RS14735 (FORC22_2787) pilM 3023082..3024098 (-) 1017 WP_071652433.1 type IV pilus biogenesis protein PilM Machinery gene
  FORC22_RS14740 (FORC22_2788) - 3024241..3026811 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  FORC22_RS14745 (FORC22_2789) oxyR 3026867..3027775 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  FORC22_RS14750 (FORC22_2790) - 3027946..3028674 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37704.25 Da        Isoelectric Point: 7.0222

>NTDB_id=161200 FORC22_RS14735 WP_071652433.1 3023082..3024098(-) (pilM) [Vibrio parahaemolyticus strain FORC_022]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKALPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=161200 FORC22_RS14735 WP_071652433.1 3023082..3024098(-) (pilM) [Vibrio parahaemolyticus strain FORC_022]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGCGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGAGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment