Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CAter10_RS18725 Genome accession   NZ_CP013233
Coordinates   4087806..4089530 (+) Length   574 a.a.
NCBI ID   WP_061534604.1    Uniprot ID   A0A127PVE6
Organism   Collimonas arenae strain Ter10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4082806..4094530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAter10_RS18710 (CAter10_4366) ispB 4085158..4086108 (+) 951 WP_128083221.1 octaprenyl diphosphate synthase -
  CAter10_RS18720 (CAter10_4368) - 4086394..4087680 (+) 1287 WP_061534603.1 HlyC/CorC family transporter -
  CAter10_RS18725 (CAter10_4369) pilB 4087806..4089530 (+) 1725 WP_061534604.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CAter10_RS18730 (CAter10_4370) pilC 4089581..4090816 (+) 1236 WP_061534605.1 type II secretion system F family protein Machinery gene
  CAter10_RS18735 (CAter10_4371) pilD 4090816..4091682 (+) 867 WP_061534606.1 prepilin peptidase Machinery gene
  CAter10_RS18740 (CAter10_4372) coaE 4091689..4092333 (+) 645 WP_061534607.1 dephospho-CoA kinase -
  CAter10_RS18745 (CAter10_4373) zapD 4092519..4093274 (+) 756 WP_061534608.1 cell division protein ZapD -
  CAter10_RS18750 (CAter10_4374) - 4093296..4093484 (+) 189 WP_061534609.1 DNA gyrase inhibitor YacG -
  CAter10_RS18755 (CAter10_4375) - 4093548..4093964 (-) 417 WP_061534610.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62692.31 Da        Isoelectric Point: 5.2943

>NTDB_id=160888 CAter10_RS18725 WP_061534604.1 4087806..4089530(+) (pilB) [Collimonas arenae strain Ter10]
MAAVFPNSSSSSPISGLARALLQAGRLSAAQVETLHKKSSGDQTPFIAALLESGHLNARDLALFCAETFGYPQLDLNTLN
LSSLPEKAIDAKLMESHSVLALAKRGNKIYVAFSDPTNMQVLDQIKFQTELTVEPIIVEHPVLLKQIQKLVKSAEQSLSE
MVGDEQEINFVEEDAAAVADAAATDIDDAPVVRFLQKILTDAINQGASDLHFEPFEKFYRIRFRVDGVLRDMAQPPLSIK
EKLASRIKVISKLDISEKRVPQDGRMRLVVSKTRAIDFRVSTLPTLFGEKIVMRILDPSQAQMGIDALGYDPDQKAILMD
AIQRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGINISTAEDPAEINLPGVNQVNINDRAGLTFPVALKAFLRQDPDIIM
VGEIRDLETADISIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVPAFNIASSVILITAQRLARRLCSCKQPISIQDEALL
EAGLTEDDIDGTWMPYKAVGCERCNGTGYKGRVGIYQIMPITEEIERIILAHGTALEIEAQAKREGVKTLRQSGLMKVKQ
GLTSLEEVLGCTNI

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=160888 CAter10_RS18725 WP_061534604.1 4087806..4089530(+) (pilB) [Collimonas arenae strain Ter10]
ATGGCAGCAGTCTTTCCGAACTCAAGTTCCAGCAGTCCGATCTCCGGACTTGCCCGTGCATTGCTCCAGGCGGGCCGCCT
TTCTGCGGCACAAGTCGAGACGCTACACAAGAAATCAAGCGGTGATCAGACGCCGTTCATTGCCGCCTTGCTGGAAAGCG
GCCACCTGAATGCACGCGACCTGGCGCTGTTTTGCGCAGAGACTTTCGGCTATCCGCAGCTGGACCTGAACACGCTGAAC
TTGAGCTCGCTGCCGGAAAAGGCGATCGACGCCAAGTTGATGGAAAGCCATTCGGTTCTGGCGCTGGCCAAGCGCGGCAA
TAAGATCTATGTCGCGTTTTCCGATCCGACCAATATGCAGGTGCTGGACCAGATCAAGTTTCAGACCGAGCTGACGGTCG
AGCCGATCATTGTCGAGCATCCGGTATTGCTGAAGCAAATCCAGAAACTGGTGAAGTCGGCCGAGCAAAGTCTCAGCGAG
ATGGTCGGCGACGAGCAGGAAATCAATTTCGTTGAAGAAGATGCCGCTGCCGTTGCCGATGCCGCGGCAACCGATATCGA
CGATGCGCCGGTGGTGCGCTTCCTGCAAAAGATATTGACCGACGCCATCAACCAGGGCGCGTCTGACTTGCATTTCGAAC
CATTCGAAAAATTCTACCGGATTCGATTCCGGGTCGATGGCGTACTGCGTGATATGGCCCAACCGCCGCTGTCCATCAAG
GAAAAACTAGCGTCGCGCATCAAGGTCATCTCCAAGCTGGATATCTCCGAAAAGCGGGTGCCGCAGGATGGCCGCATGCG
CCTGGTCGTTTCCAAGACACGCGCCATCGATTTCCGCGTCAGCACTTTGCCGACATTGTTCGGCGAAAAGATCGTCATGC
GTATCCTGGATCCGAGCCAGGCACAGATGGGGATCGATGCGCTGGGTTACGACCCGGACCAGAAAGCCATCTTGATGGAT
GCCATCCAGCGTCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCCGGCAAAACCGTATCGCTGTACACCTGCCT
GAACATCTTGAACCAGCCGGGCATCAATATCTCGACCGCCGAAGATCCGGCCGAAATCAATTTGCCCGGCGTGAACCAGG
TCAATATCAACGACCGTGCCGGACTGACATTCCCGGTCGCGCTCAAAGCTTTCCTGCGGCAGGATCCTGACATCATCATG
GTCGGTGAAATCCGCGACCTGGAAACCGCTGACATTTCGATCAAGGCGGCGCAAACCGGGCATATGGTGTTTTCCACCCT
GCATACCAACGATGCGCCGTCGACGCTGACGCGCCTGATGAACATGGGTGTGCCGGCTTTCAACATCGCCTCGTCAGTCA
TCCTGATTACCGCGCAACGACTGGCGCGGCGCCTGTGCAGCTGCAAACAGCCAATCTCGATCCAGGACGAAGCCCTGCTC
GAAGCCGGCCTGACTGAAGATGACATCGACGGCACCTGGATGCCTTACAAGGCAGTGGGCTGCGAGCGCTGCAACGGTAC
CGGCTACAAGGGGCGGGTCGGCATTTATCAAATCATGCCGATTACAGAAGAAATCGAACGCATCATCCTGGCCCACGGCA
CAGCGCTGGAAATTGAAGCGCAGGCGAAGCGCGAGGGTGTCAAAACCCTGCGTCAATCGGGGCTCATGAAAGTGAAACAA
GGGTTGACCAGCCTCGAGGAAGTGCTTGGCTGTACCAATATCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A127PVE6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.338

97.909

0.542
  pilB Acinetobacter baylyi ADP1

53.565

100

0.537
  pilF Neisseria gonorrhoeae MS11

52.406

97.735

0.512
  pilB Legionella pneumophila strain ERS1305867

50.709

98.258

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

47.08

98.432

0.463
  pilB Vibrio cholerae strain A1552

46.619

97.909

0.456
  pilB Vibrio campbellii strain DS40M4

45.775

98.955

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.265

92.16

0.371
  pilF Thermus thermophilus HB27

40.076

92.16

0.369

Multiple sequence alignment