Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   A9L45_RS20245 Genome accession   NZ_CP016625
Coordinates   3853549..3854541 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli O157:H7 strain FRIK944     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 3848549..3859541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9L45_RS20220 (A9L45_20220) pphB 3849911..3850567 (+) 657 WP_001141350.1 protein-serine/threonine phosphatase -
  A9L45_RS20225 (A9L45_20225) - 3850608..3850844 (-) 237 WP_000562982.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  A9L45_RS20230 (A9L45_20230) - 3850855..3852282 (-) 1428 WP_000863205.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  A9L45_RS20235 (A9L45_20235) - 3852282..3852875 (-) 594 WP_000767724.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  A9L45_RS20240 (A9L45_20240) - 3853022..3853429 (+) 408 WP_000175365.1 MarR family transcriptional regulator -
  A9L45_RS20245 (A9L45_20245) rpoS 3853549..3854541 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  A9L45_RS20250 (A9L45_20250) nlpD 3854604..3855698 (-) 1095 WP_306256364.1 murein hydrolase activator NlpD -
  A9L45_RS20255 (A9L45_20255) pcm 3855883..3856509 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  A9L45_RS20260 (A9L45_20260) surE 3856503..3857264 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  A9L45_RS20265 (A9L45_20265) truD 3857245..3858294 (-) 1050 WP_000568934.1 tRNA pseudouridine(13) synthase TruD -
  A9L45_RS20270 (A9L45_20270) ispF 3858291..3858770 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  A9L45_RS20275 (A9L45_20275) ispD 3858770..3859480 (-) 711 WP_000246158.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=160811 A9L45_RS20245 WP_000081550.1 3853549..3854541(-) (rpoS) [Escherichia coli O157:H7 strain FRIK944]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=160811 A9L45_RS20245 WP_000081550.1 3853549..3854541(-) (rpoS) [Escherichia coli O157:H7 strain FRIK944]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGTGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715