Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACA40_RS02045 Genome accession   NZ_CP013183
Coordinates   435559..437778 (-) Length   739 a.a.
NCBI ID   WP_003365984.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. lapsa strain ATCC 10859     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 430559..442778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA40_RS02025 (ACA40_02025) hslU 430680..432017 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  ACA40_RS02030 (ACA40_02030) hslV 432084..432614 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  ACA40_RS02035 (ACA40_02035) - 433028..433723 (-) 696 WP_004414617.1 SPOR domain-containing protein -
  ACA40_RS02040 (ACA40_02040) argS 433725..435461 (-) 1737 WP_044312621.1 arginine--tRNA ligase -
  ACA40_RS02045 (ACA40_02045) priA 435559..437778 (-) 2220 WP_003365984.1 primosomal protein N' Machinery gene
  ACA40_RS02050 (ACA40_02050) rpmE 437948..438169 (+) 222 WP_002551648.1 50S ribosomal protein L31 -
  ACA40_RS02055 (ACA40_02055) - 438183..438986 (+) 804 WP_057407104.1 thermonuclease family protein -
  ACA40_RS02060 (ACA40_02060) - 439169..440437 (+) 1269 WP_003316648.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81870.19 Da        Isoelectric Point: 8.5462

>NTDB_id=160424 ACA40_RS02045 WP_003365984.1 435559..437778(-) (priA) [Pseudomonas syringae pv. lapsa strain ATCC 10859]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=160424 ACA40_RS02045 WP_003365984.1 435559..437778(-) (priA) [Pseudomonas syringae pv. lapsa strain ATCC 10859]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCACCAGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGCCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCGATCGCCCTGCTCGACAGCGAGGCGCCGCTGCCACCTGCG
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GTTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAGGAACGCTTCTGGCACGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAAGCCCTGACCACTCTTGCCCAGCACCCGCACGGGGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAAGGACAGTCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCGCTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGGTTCGACAGCTTTCATGCGTTTCTGCTGGCCGGCGTCACAGGCAGCGGCAAGACTGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAACGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGATGCCTGGC
TGGCAGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCAGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGATCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCCTTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTTGGCTCGGCCACACCGTCCTTGGAAAGCCTGCACAACGCTTATACCG
GCCGCTACGGCCTGCTGCGCCTGAATCAGCGCGCGGGCGGCGCACAGCAGCCGCGCTTCATGCGCCTGGACGTCAAAAGC
CGACCTTTGGATAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTCTTGGT
ATTCCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGCTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAGCTGCGCTGCCATCACTGCGGGTATGTCGAGCGTGTACCGCGTCAGTGC
CCATCGTGCGGCAAAGTGGATTTGCGCCCGGTAGGCGCGGGCACAGAGCGCGCCGAAGAGCGCCTGGCGATTCTGTTTCC
GGACTACCCCGTACTGCGCGTCGATCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGTCAGCCATGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACGCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAATTAATCGTGCAGGTGGCAGG
TCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCACCCACTGCTGATCCAACTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGTTCGGCGGGTCTGCCACCGTTTTCACACCTC
GCACTGCTGCGCGCTGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCGGAGCAACT
GGTCGCGCAGATGGCGCTGGGCGGCATCGAGCTGCTGGGCCCGGTTCCGGCACCGATGGAACGCCGCGCCGGGCGTTATC
GCGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCGCCCTTGCATAAATTGCTAGCCACCTGGCTGCTCGCGCTGGAGCAA
ATGCCCAGCGGTCGGCAGGTCAGATGGTCGCTGGATGTGGATCCTGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment