Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AN416_RS00230 Genome accession   NZ_CP013102
Coordinates   53318..54838 (+) Length   506 a.a.
NCBI ID   WP_054929657.1    Uniprot ID   -
Organism   Paraburkholderia caribensis strain MWAP64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 48318..59838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AN416_RS00205 (AN416_00205) kdsD 49454..50437 (+) 984 WP_054929661.1 arabinose 5-phosphate isomerase KdsD -
  AN416_RS00210 (AN416_00210) - 50437..50997 (+) 561 WP_054929660.1 HAD family hydrolase -
  AN416_RS00215 (AN416_00215) lptC 50994..51599 (+) 606 WP_054929659.1 LPS export ABC transporter periplasmic protein LptC -
  AN416_RS00220 (AN416_00220) lptA 51621..52298 (+) 678 WP_054929658.1 lipopolysaccharide transport periplasmic protein LptA -
  AN416_RS00225 (AN416_00225) lptB 52295..53077 (+) 783 WP_060599301.1 LPS export ABC transporter ATP-binding protein -
  AN416_RS00230 (AN416_00230) rpoN/rpoN1 53318..54838 (+) 1521 WP_054929657.1 RNA polymerase factor sigma-54 Machinery gene
  AN416_RS00235 (AN416_00235) hpf 55014..55373 (+) 360 WP_012399744.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AN416_RS00240 (AN416_00240) - 55673..56188 (+) 516 WP_028365018.1 PTS sugar transporter subunit IIA -
  AN416_RS00245 (AN416_00245) hprK 56422..57390 (+) 969 WP_007579699.1 HPr(Ser) kinase/phosphatase -
  AN416_RS00250 (AN416_00250) rapZ 57468..58361 (+) 894 WP_060599302.1 RNase adapter RapZ -
  AN416_RS00255 (AN416_00255) - 58426..59061 (+) 636 WP_054929655.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55526.41 Da        Isoelectric Point: 4.8325

>NTDB_id=159903 AN416_RS00230 WP_054929657.1 53318..54838(+) (rpoN/rpoN1) [Paraburkholderia caribensis strain MWAP64]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVSMAIAQNPLLETEDDWIASPLRVAADGTVIAQTSQNTSPEEMS
GAPPSSSSSTSSGESQEGGEPQGVDEYNGMGSDANSDANQWNLDDYGRSGNASDDDDLPPLQIHESTTTLRDHLTAQLRV
TGASARDRALITFLIESLDDDGYLTATFDEILADLPEELEVDTDELNAALALLHSFDPAGVGARSASECLRLQLGRLEPS
PTRTLALDIVAHHLELLAARDFTRLRKYLKASDDDLREAHALIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELN
PEVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREI
ADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQG
FVVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=159903 AN416_RS00230 WP_054929657.1 53318..54838(+) (rpoN/rpoN1) [Paraburkholderia caribensis strain MWAP64]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCATCTTGCGCTGACACCACAACTGCAACAGTCCATCCGGCTGCTGCA
GCTGTCTACGCTCGAACTGCAACAGGAAGTGTCGATGGCGATCGCCCAGAATCCGTTGCTCGAAACGGAGGACGACTGGA
TCGCGAGTCCGTTGCGCGTCGCCGCCGACGGCACTGTCATCGCGCAGACCTCGCAGAACACGTCGCCGGAAGAGATGTCG
GGCGCGCCGCCGTCCTCGTCGTCGTCGACCTCCTCCGGCGAAAGCCAGGAAGGCGGCGAGCCACAGGGTGTGGACGAATA
CAACGGCATGGGTTCGGATGCGAACTCGGACGCCAACCAGTGGAATCTCGACGACTACGGCCGTTCGGGCAACGCATCGG
ACGACGACGATCTGCCGCCGCTGCAAATCCACGAATCGACCACGACGCTGCGCGATCACCTGACTGCTCAACTTCGCGTA
ACGGGAGCAAGCGCACGGGACCGCGCGCTCATCACGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACGGCCAC
CTTCGACGAAATCCTCGCCGATTTGCCGGAAGAACTCGAAGTCGATACCGACGAACTCAACGCGGCCCTCGCCTTGCTGC
ACAGCTTCGACCCAGCGGGGGTCGGCGCGCGTTCGGCGTCGGAATGCCTGCGTTTGCAGTTGGGCCGGCTCGAGCCGTCG
CCCACGCGCACGCTCGCGCTCGACATCGTCGCGCATCACCTGGAACTGCTCGCCGCACGCGACTTCACGCGACTGCGCAA
ATACCTGAAGGCAAGCGACGACGATCTGCGCGAAGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGTGCGGCCT
ACGGCAAGGCCGAGGCGGACTACGTCGTGCCCGACATCATGGTGCGCAAAACGGCACAGGGATGGCAGGCCGAACTGAAT
CCGGAAGTCGTGCCGAAACTGCGTATCAACCATCTGTATGCGAATATCCTGCGCAACAATCGCGGCGATCCGGGCAGCGG
TTCGCTGCGGCAGCAACTGCAGGAAGCGCGTTGGCTGATCAAGAATATCCAGCAGCGCTTCGAGACGATCCTGCGTGTCG
CACAGGCAATTGTGGAGCGTCAAAAGAACTTTTTCGCCCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATA
GCTGATACGCTGGGTTTACACGAGTCGACGGTCTCGCGTGTGACAACCGGCAAGTACATGCTCACCCCATTCGGGACGCT
TGAATTTAAGTACTTCTTCGGATCGCACGTGTCCACCGACACGGGGGGCGCGGCCTCTTCAACGGCCATTCGCGCACTCA
TCAAGCAACTGATAGGAGCGGAAGACACCAAATCTCCTCTTTCAGACAGCCGCATTGCCGAACTGCTGGCGGAACAGGGT
TTCGTCGTCGCTCGCCGTACCGTTGCGAAATACCGCGAAGCACTGAAAATCCCGGCAGTCAATCTGCGCAAGTCTCTTTA
G

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(143-333)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(349-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.996

100

0.67

Multiple sequence alignment