Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FORC37_RS13645 Genome accession   NZ_CP016321
Coordinates   2776682..2778406 (-) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain FORC_037     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2771682..2783406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC37_RS13610 (FORC37_2468) - 2772007..2772696 (-) 690 WP_088428111.1 PilW family protein -
  FORC37_RS13615 (FORC37_2469) - 2772696..2773262 (-) 567 WP_088428112.1 type IV pilus modification PilV family protein -
  FORC37_RS13620 (FORC37_2470) - 2773262..2773747 (-) 486 WP_088428113.1 type II secretion system protein -
  FORC37_RS13625 (FORC37_2471) - 2773805..2774296 (-) 492 WP_080526846.1 type II secretion system protein -
  FORC37_RS13630 (FORC37_2472) - 2774330..2774908 (-) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC37_RS13635 (FORC37_2473) - 2774987..2775448 (-) 462 WP_088428114.1 MSHA biogenesis protein MshF -
  FORC37_RS13640 (FORC37_2474) - 2775452..2776672 (-) 1221 WP_038940912.1 type II secretion system F family protein -
  FORC37_RS13645 (FORC37_2475) pilF 2776682..2778406 (-) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  FORC37_RS13650 (FORC37_2476) - 2778403..2779524 (-) 1122 WP_045594971.1 tetratricopeptide repeat protein -
  FORC37_RS13655 (FORC37_2477) - 2779521..2780369 (-) 849 WP_038940909.1 ExeA family protein -
  FORC37_RS13660 (FORC37_2478) mshL 2780377..2782023 (-) 1647 WP_088428115.1 pilus (MSHA type) biogenesis protein MshL -
  FORC37_RS13665 (FORC37_2479) - 2782039..2782359 (-) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  FORC37_RS13670 (FORC37_2480) gspM 2782352..2783002 (-) 651 WP_088428116.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=159647 FORC37_RS13645 WP_011079397.1 2776682..2778406(-) (pilF) [Vibrio vulnificus strain FORC_037]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=159647 FORC37_RS13645 WP_011079397.1 2776682..2778406(-) (pilF) [Vibrio vulnificus strain FORC_037]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGATCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCACAAAGAAGTGGTGGGCGTAAGCTTGGCGATACGCTGATTGATTTAGGTTTTTTGTCTGAGCAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCTACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGTGCATTGGTGATAGGCCGTAACGGCGACACGCTGCGCATTGCGATGAGTGA
TCCTGCCGACCTGTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAACTGGTGGAAGGCTTTGATCGCTATTATCGCCGTACCAAAGAGATCGCCAACTTTGCGGAGCAGTTGCAAGCGGAA
CACCAAGTCAACGACGCATTTGATTTCAATATTGAAGATGACGACAGTGATGAGGTAACCGTCGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATCCAGGTGGGGGCATCGGATATCCATATCGAACCGGATGCCAATGTATTGCGTTTGCGCCAGC
GTATTGATGGCGTGCTACACGAAACCTTGCTGAATGAAGTTAATATCGCGCCAGCATTGGTACTGAGATTAAAACTCATG
GCCAACTTGGATATTTCAGAAAAACGTCTGCCGCAAGATGGCCGTTTTAACATCCGATCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAATCGGTGGTGATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCAGGTTTGCCGGACGATCTTTTAGTGCGCCTACGCCGACAGTTGAAACGTCCGCACGGCATGATCCTTGTG
ACTGGCCCTACAGGCTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAGTTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAATCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACCGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTGGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGCTATCTGGTTGCGAGTGCTGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAAACAGAAGATCACGTTGGCGAGTCACGTAAAGCGTGGTTGGCACAGCGATTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGTCAAAATTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCGCATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGCTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371