Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ASJ33_RS00885 Genome accession   NZ_CP013074
Coordinates   166358..167746 (+) Length   462 a.a.
NCBI ID   WP_012881409.1    Uniprot ID   A0A0C6EMF5
Organism   Dehalococcoides mccartyi strain CG3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 161358..172746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASJ33_RS00875 (ASJ33_00865) - 163309..165783 (+) 2475 WP_023651721.1 ATP-dependent Clp protease ATP-binding subunit -
  ASJ33_RS00880 (ASJ33_00870) - 165906..166295 (+) 390 WP_023651722.1 hypothetical protein -
  ASJ33_RS00885 (ASJ33_00875) radA 166358..167746 (+) 1389 WP_012881409.1 DNA repair protein RadA Machinery gene
  ASJ33_RS00890 (ASJ33_00880) - 167733..168416 (+) 684 WP_023651724.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  ASJ33_RS00895 (ASJ33_00885) ispF 168422..168895 (+) 474 WP_023651725.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  ASJ33_RS00900 (ASJ33_00890) cysS 168899..170272 (+) 1374 WP_041330349.1 cysteine--tRNA ligase -
  ASJ33_RS00910 (ASJ33_00900) - 170490..171449 (+) 960 WP_041330381.1 tyrosine-type recombinase/integrase -
  ASJ33_RS00915 (ASJ33_00905) - 171451..171972 (-) 522 WP_041330383.1 hypothetical protein -
  ASJ33_RS08385 - 172170..172343 (+) 174 WP_155760387.1 hypothetical protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 49476.80 Da        Isoelectric Point: 7.2840

>NTDB_id=159568 ASJ33_RS00885 WP_012881409.1 166358..167746(+) (radA) [Dehalococcoides mccartyi strain CG3]
MEKSRRVYVCSNCGRESLKWLGRCPGCQEWNTLEETTVVSPVGRKNEPARVISPAAELSSLQAAETTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLSPSLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQTNTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDIGVCPETIALGEIGLSGEMR
TISHLERRLSEASRLGFKRALVPAGANCHNVHNIQIIPVSTVKEAVRLALTGVKTETEDVFE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=159568 ASJ33_RS00885 WP_012881409.1 166358..167746(+) (radA) [Dehalococcoides mccartyi strain CG3]
ATGGAAAAAAGCCGTAGAGTTTATGTTTGTTCCAACTGCGGGCGTGAGAGCCTGAAGTGGCTGGGGCGCTGTCCCGGCTG
TCAGGAATGGAATACCCTTGAAGAAACAACGGTTGTTTCACCTGTAGGACGTAAAAATGAACCTGCCCGTGTTATCAGCC
CGGCGGCTGAGCTTTCCAGTCTGCAGGCAGCCGAAACCACCCGGCGGAGTTTATCTATTTCGGAGTTCAACCGGGTATTA
GGCGGCGGCATAGTGCCGGGTTCGCTTATGCTTTTGGGTGGTGAGCCGGGAATCGGCAAATCCACTCTTCTTCTTCAGGT
GGCGGCTTCGGTAGCACAAAGCGGCGGTAAGGTAGTATATGTTTCAGGGGAAGAAAACCCCGCTCAGATAAAAATGCGCG
CCCAACGTCTTGGTATCAGCGGCGAAGGGCTTTTCCTTATGGCTGAGACCGACCTTAATGCCATTCTGGCTCAGCTTTCA
GTCCTCAGCCCGTCATTGGTCGTTATTGACTCTATCCAAACTGTATTCCTGCCGGAGCTTGAGGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAATCAGCCCTCCGCCTTATGCAGTGGGCTAAAAGCAGCGGTGCCAGTGTATTTATAACAGCCCATG
TTACTAAAGAAGGCAATATAGCCGGGCCGCGCATATTGGAACACATAGTAGATGTAGTCATGTACTTTGAGGGAGAGTCC
CAGAGTGCTTACCGTTTAGTGCGTTCTGTCAAAAACCGCTTCGGTTCCACTAACGAAGTGGGTATATTTGAAATGAAAAG
TGAAGGTCTGGTAGAAGTAGCTAACCCTTCCCAGATATTTTTATCAAATCGCCAGACAAACACGGTAGGCTCGGCTGTAA
CCGCTGTACTGGAGGGCAGCCGTCCTTTGCTGGTTGAGGTGCAGGCACTTACCAATACCACCAGTTTCGGCCAGCCGCGC
CGCACTGCCAACGGGGTGGATTTTAACCGCACCCTTATGATAGCTGCGGTCCTCTCCAAACGCCTGTCCATGCGTTTAGG
TACGCAGGACATTATTGTAAACGCCACCGGCGGTATCCGTTTGGATGAGCCGGCTGCAGATTTGGCTATTGCACTGGCTA
TTGCTTCCAGTTACCGGGATATCGGCGTATGCCCCGAAACTATCGCTCTGGGAGAGATTGGTCTTTCTGGGGAAATGCGG
ACTATTTCCCATCTGGAAAGGCGTCTTTCCGAGGCCAGCCGTTTGGGTTTTAAAAGGGCTTTGGTACCGGCCGGTGCTAA
CTGCCACAATGTACATAATATCCAGATAATTCCGGTTTCAACTGTCAAAGAGGCAGTGAGGCTGGCACTTACCGGGGTGA
AAACGGAGACGGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(342-424)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C6EMF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.109

99.351

0.498
  radA Streptococcus pneumoniae Rx1

48.14

98.918

0.476
  radA Streptococcus pneumoniae D39

48.14

98.918

0.476
  radA Streptococcus pneumoniae R6

48.14

98.918

0.476
  radA Streptococcus pneumoniae TIGR4

48.14

98.918

0.476
  radA Streptococcus mitis SK321

48.14

98.918

0.476
  radA Streptococcus mitis NCTC 12261

47.921

98.918

0.474

Multiple sequence alignment