Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NI40_RS19810 Genome accession   NZ_CP012999
Coordinates   4243178..4244611 (+) Length   477 a.a.
NCBI ID   WP_023309360.1    Uniprot ID   A0A1Z3N130
Organism   Enterobacter sp. E20     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4238178..4249611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI40_RS19780 (NI40_019775) - 4238745..4239722 (+) 978 WP_039260412.1 calcium/sodium antiporter -
  NI40_RS19785 (NI40_019780) kdsD 4239736..4240722 (+) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  NI40_RS19790 (NI40_019785) kdsC 4240737..4241303 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NI40_RS19795 (NI40_019790) lptC 4241300..4241875 (+) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  NI40_RS19800 (NI40_019795) lptA 4241844..4242398 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NI40_RS19805 (NI40_019800) lptB 4242405..4243130 (+) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  NI40_RS19810 (NI40_019805) rpoN/rpoN1 4243178..4244611 (+) 1434 WP_023309360.1 RNA polymerase factor sigma-54 Machinery gene
  NI40_RS19815 (NI40_019810) hpf 4244634..4244921 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  NI40_RS19820 (NI40_019815) ptsN 4245004..4245495 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NI40_RS19825 (NI40_019820) rapZ 4245541..4246395 (+) 855 WP_014885397.1 RNase adapter RapZ -
  NI40_RS19830 (NI40_019825) npr 4246392..4246664 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  NI40_RS19835 (NI40_019830) mtgA 4246704..4247429 (-) 726 WP_039260411.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NI40_RS19840 (NI40_019835) elbB 4247426..4248079 (-) 654 WP_039260518.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=159075 NI40_RS19810 WP_023309360.1 4243178..4244611(+) (rpoN/rpoN1) [Enterobacter sp. E20]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=159075 NI40_RS19810 WP_023309360.1 4243178..4244611(+) (rpoN/rpoN1) [Enterobacter sp. E20]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAAATTGAGCTTGAGGAAGTCGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTATCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATTATTAGCGATCATCTGGATCTGCTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTAAATCTGATTCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTGAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGTGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N130

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment