Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A9Z04_RS09990 Genome accession   NZ_CP016007
Coordinates   2062711..2064192 (+) Length   493 a.a.
NCBI ID   WP_001219890.1    Uniprot ID   -
Organism   Escherichia coli strain NGF1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2057711..2069192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9Z04_RS09970 (A9Z04_09970) gspB 2057880..2058299 (-) 420 WP_000461443.1 putative general secretion pathway protein GspB -
  A9Z04_RS09975 (A9Z04_09975) gspA 2058301..2059770 (-) 1470 WP_000107592.1 type II secretion system protein GspA -
  A9Z04_RS09980 (A9Z04_09980) gspC 2059950..2060765 (+) 816 WP_001142675.1 type II secretion system protein GspC -
  A9Z04_RS09985 (A9Z04_09985) gspD 2060749..2062701 (+) 1953 WP_001520228.1 type II secretion system secretin GspD -
  A9Z04_RS09990 (A9Z04_09990) pilB 2062711..2064192 (+) 1482 WP_001219890.1 type II secretion system ATPase GspE Machinery gene
  A9Z04_RS09995 (A9Z04_09995) gspF 2064189..2065385 (+) 1197 WP_032290726.1 type II secretion system inner membrane protein GspF -
  A9Z04_RS10000 (A9Z04_10000) gspG 2065395..2065832 (+) 438 WP_001202878.1 type II secretion system major pseudopilin GspG -
  A9Z04_RS10005 (A9Z04_10005) gspH 2065840..2066349 (+) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  A9Z04_RS10010 (A9Z04_10010) gspI 2066346..2066723 (+) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  A9Z04_RS10015 (A9Z04_10015) gspJ 2066716..2067303 (+) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  A9Z04_RS10020 (A9Z04_10020) gspK 2067296..2068279 (+) 984 WP_001058508.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54690.66 Da        Isoelectric Point: 6.2412

>NTDB_id=158495 A9Z04_RS09990 WP_001219890.1 2062711..2064192(+) (pilB) [Escherichia coli strain NGF1]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFAPVSPQQAQMFK
YHQLVVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=158495 A9Z04_RS09990 WP_001219890.1 2062711..2064192(+) (pilB) [Escherichia coli strain NGF1]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAAGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGACTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCCAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATCAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCATCCATCTATGGTGAACGCGCTGTGCTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTAACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATTCTTTCGGCGCTTAA
TACCCCTGGCCGTAATATTCTGACGGTAGAAGATCCCGTGGAATATGAACTGGAAGGCATAGGACAAACGCAGGTGAATA
CCCGCGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAGATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACAGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTCACCCGGCTACGCGACATGGGCGTTGAATCATTCCTGCTTTCGTCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCGCGCCCGTATCCCCTCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGTGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.364

89.249

0.414
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.682

89.249

0.408
  pilB Legionella pneumophila strain ERS1305867

41.58

97.566

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

46.698

86.004

0.402
  pilB/pilB1 Synechocystis sp. PCC 6803

41.202

94.523

0.389
  pilB Acinetobacter baylyi ADP1

48.338

79.31

0.383
  pilF Thermus thermophilus HB27

45.063

80.122

0.361

Multiple sequence alignment