Detailed information NTG0158459

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FORC23_RS01940
• Genome accession: NZ_CP012950
• Coordinates: 400751..401767 (+)
• Length: 338 a.a.
• NCBI ID: WP_005496568.1
• Uniprot ID: A0A3E1IL27
• Organism: Vibrio parahaemolyticus strain FORC_023 isolate 378
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 395751..406767

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FORC23_RS01925 (FORC23_0328)	-	396175..396903 (-)	729	WP_070225480.1	redoxin family protein	-
FORC23_RS01930 (FORC23_0329)	oxyR	397074..397982 (+)	909	WP_005465160.1	DNA-binding transcriptional regulator OxyR	-
FORC23_RS01935 (FORC23_0330)	-	398038..400608 (-)	2571	WP_005496567.1	penicillin-binding protein 1A	-
FORC23_RS01940 (FORC23_0331)	pilM	400751..401767 (+)	1017	WP_005496568.1	type IV pilus biogenesis protein PilM	Machinery gene
FORC23_RS01945 (FORC23_0332)	pilN	401751..402329 (+)	579	WP_015297396.1	PilN domain-containing protein	Machinery gene
FORC23_RS01950 (FORC23_0333)	pilO	402322..402915 (+)	594	WP_029806468.1	type 4a pilus biogenesis protein PilO	Machinery gene
FORC23_RS01955 (FORC23_0334)	pilP	402905..403420 (+)	516	WP_025442304.1	pilus assembly protein PilP	Machinery gene
FORC23_RS01960 (FORC23_0335)	pilQ	403452..405200 (+)	1749	WP_005488227.1	type IV pilus secretin PilQ	Machinery gene
FORC23_RS01965 (FORC23_0336)	aroK	405389..405907 (+)	519	WP_005381319.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37762.28 Da
• Isoelectric Point: 6.8323

>NTDB_id=158459 FORC23_RS01940 WP_005496568.1 400751..401767(+) (pilM) [Vibrio parahaemolyticus strain FORC_023 isolate 378]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide

• Length: 1017 bp

>NTDB_id=158459 FORC23_RS01940 WP_005496568.1 400751..401767(+) (pilM) [Vibrio parahaemolyticus strain FORC_023 isolate 378]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCACAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-326)

3D structure

• AlphaFold DB: A0A3E1IL27

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	85.207	100	0.852
pilM	Vibrio cholerae strain A1552	48.368	99.704	0.482