Detailed information NTG0158428

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: APZ20_RS15565
• Genome accession: NZ_CP012947
• Coordinates: 3336730..3338121 (+)
• Length: 463 a.a.
• NCBI ID: WP_011258418.1
• Uniprot ID: A0A854CLX6
• Organism: Xanthomonas oryzae pv. oryzae strain PXO83
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	3335682..3362065	3336730..3338121	within	0

Gene organization within MGE regions

Location: 3335682..3362065

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
APZ20_RS15565 (APZ20_15100)	radA	3336730..3338121 (+)	1392	WP_011258418.1	DNA repair protein RadA	Machinery gene
APZ20_RS15570 (APZ20_15105)	-	3338502..3339152 (-)	651	Protein_2945	transposase	-
APZ20_RS15575 (APZ20_15110)	-	3339222..3339401 (+)	180	Protein_2946	transposase	-
APZ20_RS15580 (APZ20_15115)	-	3339492..3340448 (+)	957	WP_041182545.1	IS30-like element IS1112b family transposase	-
APZ20_RS15585 (APZ20_15120)	-	3340448..3341381 (+)	934	Protein_2948	IS3 family transposase	-
APZ20_RS15590 (APZ20_15125)	mutS	3341635..3344190 (+)	2556	WP_011408105.1	DNA mismatch repair protein MutS	-
APZ20_RS15595 (APZ20_15130)	-	3344247..3344450 (+)	204	WP_012445372.1	hypothetical protein	-
APZ20_RS15600 (APZ20_15135)	-	3344447..3346039 (+)	1593	Protein_2951	T6SS effector BTH_I2691 family protein	-
APZ20_RS25270	-	3346604..3346605 (-)	2	WP_094187728.1	IS5 family transposase	-
APZ20_RS15610 (APZ20_15145)	-	3346918..3348019 (-)	1102	Protein_2953	IS3 family transposase	-
APZ20_RS15620 (APZ20_15155)	-	3347919..3349730 (-)	1812	Protein_2954	type IV secretion protein Rhs	-
APZ20_RS15625 (APZ20_15160)	-	3349773..3350216 (-)	444	WP_230455536.1	DUF4303 domain-containing protein	-
APZ20_RS15630 (APZ20_15165)	-	3350355..3351311 (+)	957	WP_041182545.1	IS30-like element IS1112b family transposase	-
APZ20_RS15635 (APZ20_15170)	-	3351387..3352355 (-)	969	WP_044756968.1	IS5-like element ISXo1 family transposase	-
APZ20_RS29080	-	3352354..3352968 (+)	615	WP_143690773.1	RHS repeat-associated core domain-containing protein	-
APZ20_RS15640 (APZ20_15175)	-	3353020..3353988 (+)	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
APZ20_RS15650 (APZ20_15185)	-	3354546..3355865 (-)	1320	WP_109182069.1	IS701-like element ISXo15 family transposase	-
APZ20_RS15655 (APZ20_15190)	-	3356065..3357033 (-)	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
APZ20_RS15660 (APZ20_15195)	-	3357170..3358489 (+)	1320	WP_115862281.1	IS701-like element ISXo15 family transposase	-
APZ20_RS15665 (APZ20_15200)	-	3358556..3360826 (-)	2271	Protein_2963	type IV secretion protein Rhs	-
APZ20_RS15670 (APZ20_15205)	-	3361109..3362065 (+)	957	WP_041182545.1	IS30-like element IS1112b family transposase	-

Sequence

Protein

• Length: 463 a.a.
• Molecular weight: 48847.13 Da
• Isoelectric Point: 7.3924

>NTDB_id=158428 APZ20_RS15565 WP_011258418.1 3336730..3338121(+) (radA) [Xanthomonas oryzae pv. oryzae strain PXO83]
MAKAKTAYVCGECGAEYTKWQGQCTECGVWNTLSEIVLESATPGGKASPATSRRIGWAGKAEAPKITALKDVQQSEQARV
STGIGEFDRVLGGGLVEGAVVLIGGDPGIGKSTLLLQALASMASTLPVLYVTGEESLAQVAGRAVRLDLPLDGLNALSET
GIEHILQHASVARPRLIVADSVQTLWTESLTAAPGSVSQVRESAARLVRYAKETGTAVFLVGHVTKEGGIAGPRVLEHMV
DAVLYFEGESGSRFRLLRAFKNRFGAVNELGVFAMGEKGLKEVSNPSAIFLSGGSTQQPGSCVMVTREGTRPLMVEVQAL
VDASPLSNPRRVAVGLEQNRLAMLLAVLHRHGGIVVGDQDVFVNVVGGIRVQETAADLPVLLAVLSSLRDRPLAEKTIAF
GEVGLSGEIRPVPNGEDRLKEAATHGFKRAIVPRANAPKTTSIKGMEIIAVERLRQALEVAAD

Nucleotide

• Length: 1392 bp

>NTDB_id=158428 APZ20_RS15565 WP_011258418.1 3336730..3338121(+) (radA) [Xanthomonas oryzae pv. oryzae strain PXO83]
ATGGCGAAGGCGAAGACGGCCTACGTGTGCGGGGAGTGCGGTGCCGAGTACACAAAGTGGCAGGGGCAGTGCACCGAGTG
CGGTGTCTGGAACACGCTGAGCGAAATTGTGCTCGAAAGCGCAACGCCGGGCGGCAAGGCCTCGCCTGCGACCTCGCGGC
GTATCGGTTGGGCCGGCAAGGCCGAGGCGCCCAAGATCACTGCGCTCAAGGACGTGCAGCAGTCCGAGCAGGCGCGCGTG
TCCACCGGCATCGGCGAGTTCGACCGGGTGTTGGGCGGCGGGTTGGTCGAGGGGGCGGTGGTGCTGATCGGCGGCGACCC
GGGCATCGGCAAATCGACGCTGCTGCTGCAGGCGCTGGCGAGCATGGCCTCTACGTTGCCGGTGTTGTACGTCACCGGCG
AAGAATCGCTGGCCCAGGTAGCAGGGCGCGCGGTGCGCCTGGATCTGCCGCTGGACGGCCTGAACGCCCTGTCCGAAACC
GGTATCGAACACATCCTGCAGCACGCCAGCGTGGCGCGGCCGAGGCTGATCGTGGCCGATTCGGTGCAGACCTTGTGGAC
CGAATCGCTTACCGCAGCGCCAGGCTCGGTGAGCCAGGTGCGCGAGAGCGCAGCGCGGCTGGTGCGCTACGCCAAGGAAA
CCGGCACCGCCGTGTTTCTGGTCGGCCATGTGACCAAGGAAGGCGGTATCGCCGGGCCACGCGTGCTTGAGCACATGGTC
GATGCGGTGCTGTATTTCGAAGGCGAAAGTGGCAGCCGATTCCGCCTGTTGCGCGCGTTCAAGAACCGCTTCGGTGCGGT
CAACGAGCTAGGCGTCTTCGCGATGGGCGAGAAGGGCCTCAAGGAAGTCTCCAACCCATCGGCAATCTTCCTGTCCGGTG
GCAGCACTCAGCAACCGGGCAGTTGCGTGATGGTCACCCGCGAAGGCACCCGCCCCCTGATGGTGGAGGTGCAGGCCTTG
GTGGATGCCTCGCCGTTGTCCAATCCGCGCCGCGTTGCGGTGGGGCTGGAGCAGAACCGGCTGGCGATGTTGCTGGCGGT
CCTGCACCGCCATGGCGGCATCGTGGTCGGCGATCAGGACGTGTTCGTCAATGTGGTGGGTGGCATCCGCGTGCAGGAAA
CGGCGGCCGATCTGCCCGTGCTGCTGGCGGTGCTGTCGTCGCTGCGCGACCGGCCGTTGGCCGAAAAGACCATCGCGTTC
GGCGAAGTGGGACTGTCCGGCGAAATCCGCCCGGTGCCCAACGGCGAGGACCGCCTGAAGGAAGCGGCGACGCATGGCTT
CAAGCGCGCCATTGTGCCCCGCGCCAATGCGCCCAAGACGACCAGCATCAAGGGAATGGAAATTATCGCAGTGGAGCGCT
TGAGACAGGCGCTGGAGGTGGCGGCAGACTGA

Domains

Predicted by InterproScan.

• AAA domain (AAA_25) (90-228)

• Subunit ChlI of Mg-chelatase (ChlI) (352-436)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

3D structure

• AlphaFold DB: A0A854CLX6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	48.38	100	0.484
radA	Streptococcus mitis NCTC 12261	42.484	99.136	0.421
radA	Streptococcus pneumoniae Rx1	42.484	99.136	0.421
radA	Streptococcus pneumoniae D39	42.484	99.136	0.421
radA	Streptococcus pneumoniae R6	42.484	99.136	0.421
radA	Streptococcus pneumoniae TIGR4	42.484	99.136	0.421
radA	Streptococcus mitis SK321	42.266	99.136	0.419