Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RALBFv3_RS11170 Genome accession   NZ_CP012943
Coordinates   2463362..2464861 (-) Length   499 a.a.
NCBI ID   WP_014618027.1    Uniprot ID   F6G492
Organism   Ralstonia solanacearum strain IBSBF1503     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2458362..2469861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RALBFv3_RS11140 (RALBFv3_11135) - 2458869..2459522 (-) 654 WP_014618031.1 LON peptidase substrate-binding domain-containing protein -
  RALBFv3_RS11145 (RALBFv3_11140) rapZ 2459600..2460490 (-) 891 WP_014618030.1 RNase adapter RapZ -
  RALBFv3_RS11150 (RALBFv3_11145) - 2460584..2460916 (-) 333 WP_039550978.1 PsiF family protein -
  RALBFv3_RS11155 (RALBFv3_11150) hprK 2461107..2462081 (-) 975 WP_003262273.1 HPr(Ser) kinase/phosphatase -
  RALBFv3_RS11160 (RALBFv3_11155) ptsN 2462152..2462607 (-) 456 WP_003262274.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RALBFv3_RS11165 (RALBFv3_11160) hpf 2462957..2463310 (-) 354 WP_003262275.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RALBFv3_RS11170 (RALBFv3_11165) rpoN/rpoN1 2463362..2464861 (-) 1500 WP_014618027.1 RNA polymerase factor sigma-54 Machinery gene
  RALBFv3_RS11175 (RALBFv3_11170) lptB 2464959..2465747 (-) 789 WP_014618026.1 LPS export ABC transporter ATP-binding protein -
  RALBFv3_RS11180 (RALBFv3_11175) lptA 2465781..2466377 (-) 597 WP_014618025.1 lipopolysaccharide transport periplasmic protein LptA -
  RALBFv3_RS11185 (RALBFv3_11180) lptC 2466445..2467077 (-) 633 WP_014618024.1 LPS export ABC transporter periplasmic protein LptC -
  RALBFv3_RS11190 (RALBFv3_11185) - 2467079..2467666 (-) 588 WP_014618023.1 HAD family hydrolase -
  RALBFv3_RS11195 (RALBFv3_11190) - 2467699..2468682 (-) 984 WP_014618022.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55158.56 Da        Isoelectric Point: 4.8522

>NTDB_id=158394 RALBFv3_RS11170 WP_014618027.1 2463362..2464861(-) (rpoN/rpoN1) [Ralstonia solanacearum strain IBSBF1503]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSNSATPPPTE
SPPPAANGSDHADTAEASYENGSDFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSMR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLESPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=158394 RALBFv3_RS11170 WP_014618027.1 2463362..2464861(-) (rpoN/rpoN1) [Ralstonia solanacearum strain IBSBF1503]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTGGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGATGGTTCGGTCAGCGCGCTGCAGAGCAACAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCTCGGACCACGCCGACACAGCCGAAGCATCGTACGAAAACGGCAGCGACTTCGACAG
CGACTACGGCTCCGATCGCTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCCCAGTCCGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAGCCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAATTGTCCATGCGT
GACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGACGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGGCCG
GCATCGGTGCACGCTCGGCTGCGGAATGCCTGACGCTGCAGTTGCAGCGCCTGGAGTCGCCAGCCAAGCCGCTCGCGCTG
GCCATCGTCAACCAGCATCTCGAGCTGCTCGCCGCGCGCGATTACACGCGCCTGAAGAAAGCGCTGTCCGTCGACGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCGCCGTTCCCGGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TCGTCGTGCCCGATGTCGTGGTGCGTAAAACCGCCGCAGGCTGGATGGCGCAACTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGCGGCGAGTCGGGCGCCGCGAACCTGCAGCAAAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCAGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6G492

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.595

100

0.976

Multiple sequence alignment