Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AO356_RS15700 Genome accession   NZ_CP012831
Coordinates   3548718..3550085 (+) Length   455 a.a.
NCBI ID   WP_060740535.1    Uniprot ID   A0A1I5J9C4
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3543718..3555085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS15675 (AO356_15655) - 3543954..3544694 (-) 741 WP_237140818.1 autoinducer binding domain-containing protein -
  AO356_RS15680 (AO356_15660) - 3544959..3545735 (+) 777 WP_060740533.1 ferredoxin--NADP reductase -
  AO356_RS15685 (AO356_15665) mscL 3545738..3546151 (-) 414 WP_003205779.1 large-conductance mechanosensitive channel protein MscL -
  AO356_RS15690 (AO356_15670) katB 3546435..3547976 (+) 1542 WP_060740534.1 catalase KatB -
  AO356_RS15695 (AO356_15675) - 3548127..3548675 (+) 549 WP_060743128.1 ankyrin repeat domain-containing protein -
  AO356_RS15700 (AO356_15680) radA 3548718..3550085 (+) 1368 WP_060740535.1 DNA repair protein RadA Machinery gene
  AO356_RS15705 (AO356_15685) - 3550224..3550580 (-) 357 WP_060740536.1 PilZ domain-containing protein -
  AO356_RS15710 (AO356_15690) - 3550739..3552805 (+) 2067 WP_060740537.1 carbon starvation CstA family protein -
  AO356_RS15715 (AO356_15695) - 3552821..3553018 (+) 198 WP_003182142.1 YbdD/YjiX family protein -
  AO356_RS15720 (AO356_15700) yjiA 3553310..3554278 (+) 969 WP_060740538.1 GTPase -
  AO356_RS15725 (AO356_15705) - 3554270..3554983 (-) 714 WP_060740539.1 GntR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48786.25 Da        Isoelectric Point: 6.8987

>NTDB_id=157819 AO356_RS15700 WP_060740535.1 3548718..3550085(+) (radA) [Pseudomonas fluorescens strain FW300-N2C3]
MAKAKRMYGCTECGSTFPKWAGQCSECGAWNTLTETMVESGGAAAPTGRTGWAGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLATRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=157819 AO356_RS15700 WP_060740535.1 3548718..3550085(+) (radA) [Pseudomonas fluorescens strain FW300-N2C3]
ATGGCCAAGGCAAAGCGCATGTACGGCTGCACCGAGTGCGGTTCGACCTTTCCCAAATGGGCCGGCCAATGCAGCGAATG
CGGGGCCTGGAACACCCTGACCGAAACCATGGTGGAAAGCGGCGGCGCGGCGGCCCCCACCGGGCGCACCGGCTGGGCCG
GTCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGCGTTGAAGAAATCCCGCGATTTTCCACCGCGTCCGGTGAACTG
GACCGGGTGCTGGGCGGCGGCCTGGTGGACGGTTCGGTGGTGCTGATCGGTGGAGACCCGGGCATCGGCAAATCCACCAT
CCTGCTGCAAACCTTGTGCAACCTCGCCACCCGCATGCCGGCGCTGTACGTCACTGGCGAAGAGTCCCAGCAACAGGTCG
CCATGCGCGCCCGGCGCCTGGGCTTGCCCCAGGACCAGTTGCGGGTCATGACCGAGACCTGCATCGAAACCATCATCGCC
ACGGCGCGCGTGGAAAAGCCCAAGGTGATGGTGATCGACTCGATCCAGACCATTTTCACCGAACAGCTGCAATCGGCCCC
GGGCGGCGTGTCCCAGGTGCGTGAAAGCGCGGCGTTGCTGGTGCGCTACGCCAAGCAGAGCGGCACGGCGATTTTCCTGG
TGGGCCACGTCACCAAGGAGGGTGCGTTGGCGGGACCGCGGGTGTTGGAGCACATGGTCGATACCGTGTTGTACTTCGAA
GGCGAATCCGATGGCCGCCTGCGTTTGCTCCGGGCGGTGAAGAACCGTTTTGGTGCCGTCAATGAATTGGGCGTGTTCGG
CATGACCGACAGGGGCCTGAAAGAAGTCTCGAACCCTTCGGCGATCTTTCTGACGCGTGCCCAGGAAGAAGTCCCGGGCA
GCGTGGTCATGGCGACGTGGGAAGGGACCCGGCCGATGTTGGTGGAAGTGCAGGCGCTGGTGGATGACAGCCACTTGGCG
AACCCACGGCGGGTCACCCTGGGCCTGGATCAGAATCGGCTGGCCATGCTGCTGGCGGTGTTGCACCGACACGGCGGGAT
TCCGACTCACGACCAGGACGTGTTCCTCAACGTCGTGGGCGGGGTCAAGGTACTGGAGACCGCGTCCGACCTGGCGTTGA
TGGCGGCGGTCATGTCCAGCCTGCGCAACCGGCCGCTGCCCCATGATTTGCTGGTGTTTGGCGAAGTGGGGTTGTCGGGG
GAAGTGCGACCGGTGCCCAGCGGGCAGGAGCGGCTCAAGGAAGCGGCCAAGCACGGTTTCAAACGCGCCATCGTGCCCAA
GGGCAACGCGCCGAAGGAAGCGCCGCCGGGGTTGCAGATTATCGCAGTGACCCGTCTGGAACAGGCGTTGGATGCGCTGT
TCGAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(80-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I5J9C4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.015

100

0.492
  radA Streptococcus pneumoniae Rx1

46.304

100

0.468
  radA Streptococcus pneumoniae R6

46.304

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.304

100

0.468
  radA Streptococcus pneumoniae D39

46.304

100

0.468
  radA Streptococcus mitis SK321

46.491

100

0.466
  radA Streptococcus mitis NCTC 12261

46.272

100

0.464

Multiple sequence alignment