Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AO353_RS13980 Genome accession   NZ_CP012830
Coordinates   3104838..3106205 (+) Length   455 a.a.
NCBI ID   WP_019691594.1    Uniprot ID   A0A1J0EMP0
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3099838..3111205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS13955 (AO353_13955) - 3100053..3100769 (-) 717 WP_054595492.1 autoinducer binding domain-containing protein -
  AO353_RS13960 (AO353_13960) - 3101113..3101889 (+) 777 WP_054595493.1 ferredoxin--NADP reductase -
  AO353_RS13965 (AO353_13965) mscL 3101935..3102348 (-) 414 WP_019691591.1 large-conductance mechanosensitive channel protein MscL -
  AO353_RS13970 (AO353_13970) katB 3102633..3104174 (+) 1542 WP_054595494.1 catalase KatB -
  AO353_RS13975 (AO353_13975) - 3104238..3104792 (+) 555 WP_054595495.1 ankyrin repeat domain-containing protein -
  AO353_RS13980 (AO353_13980) radA 3104838..3106205 (+) 1368 WP_019691594.1 DNA repair protein RadA Machinery gene
  AO353_RS13985 (AO353_13985) - 3106240..3106620 (-) 381 WP_054595496.1 PilZ domain-containing protein -
  AO353_RS13990 (AO353_13990) - 3106779..3108845 (+) 2067 WP_054595497.1 carbon starvation CstA family protein -
  AO353_RS13995 (AO353_13995) - 3108861..3109058 (+) 198 WP_010448516.1 YbdD/YjiX family protein -
  AO353_RS14000 (AO353_14000) yjiA 3109181..3110152 (+) 972 WP_054595498.1 GTPase -
  AO353_RS14005 (AO353_14005) - 3110238..3110960 (-) 723 WP_054595499.1 GntR family transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48698.17 Da        Isoelectric Point: 7.1317

>NTDB_id=157784 AO353_RS13980 WP_019691594.1 3104838..3106205(+) (radA) [Pseudomonas fluorescens strain FW300-N2E3]
MAKAKRMYGCTECGSTFPKWAGQCGECGAWNTLTETLVESGGAAAPSGRTGWAGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLATRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLRIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=157784 AO353_RS13980 WP_019691594.1 3104838..3106205(+) (radA) [Pseudomonas fluorescens strain FW300-N2E3]
ATGGCCAAGGCAAAGCGCATGTACGGCTGCACCGAATGCGGCTCGACCTTCCCCAAGTGGGCCGGCCAATGCGGTGAATG
CGGGGCCTGGAACACCCTGACCGAAACCCTGGTGGAAAGTGGCGGTGCCGCTGCCCCCAGCGGTCGCACCGGCTGGGCCG
GACAGCAGGCGCAGATCAAGACGCTGGCCGAAGTCAGTGTCGAAGAAATTCCACGCTTCTCCACCGCGTCCGGTGAGCTG
GATCGGGTGCTCGGTGGCGGTCTGGTCGACGGCTCGGTGGTGTTGATCGGCGGTGATCCGGGGATCGGCAAGTCGACGAT
CCTTCTGCAAACCCTGTGCAACCTCGCGACCCGGATGCCGGCGCTGTATGTCACGGGCGAAGAGTCCCAGCAGCAAGTGG
CCATGCGCGCTCGACGTCTGGGCTTGCCGCAGGATCAACTGCGAGTCATGACCGAAACCTGCATCGAAACCATCATCGCC
ACGGCTCGGGTGGAAAAGCCCAAGGTCATGGTGATCGACTCGATCCAGACGATTTTCACCGAACAACTGCAATCGGCGCC
GGGCGGCGTATCGCAGGTTCGCGAAAGTGCAGCGCTGTTGGTGCGTTACGCGAAACAGAGCGGCACGGCGATTTTCCTCG
TCGGCCACGTGACCAAAGAAGGCGCGTTGGCGGGGCCCCGAGTGCTGGAACACATGGTCGACACGGTGCTGTATTTCGAA
GGCGAGTCGGATGGACGCTTGCGTCTGTTGCGAGCGGTGAAAAACCGCTTCGGTGCGGTCAACGAGTTGGGTGTGTTCGG
TATGACCGACAAGGGTTTGAAGGAGGTCTCCAACCCTTCGGCGATTTTCCTCACACGCGCGCAGGAAGAAGTGCCGGGCA
GCGTCGTGATGGCGACGTGGGAAGGCACGCGACCGATGCTGGTGGAAGTTCAGGCTCTGGTGGACGACAGCCACCTGGCC
AACCCGCGCCGTGTCACCCTCGGTCTGGATCAGAATCGCCTGGCCATGCTGTTGGCGGTGCTGCACCGTCATGGAGGCAT
ACCGACTCACGACCAGGACGTGTTCCTCAACGTGGTCGGCGGGGTCAAGGTGCTGGAGACGGCGTCCGATCTGGCGTTGA
TGGCAGCCGTGATGTCCAGCCTGCGCAATCGGCCATTGCCACATGATCTGTTGGTGTTTGGTGAGGTTGGCTTGTCTGGT
GAAGTGCGGCCGGTGCCAAGCGGTCAGGAGCGCTTGAAGGAAGCCGCCAAGCACGGCTTCAAACGCGCCATCGTGCCCAA
GGGCAATGCGCCGAAGGAAGCGCCTGCCGGGTTACGGATCATCGCGGTGACGCGACTGGAACAGGCCTTGGACGCGTTAT
TTGAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(80-221)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J0EMP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.796

100

0.49
  radA Streptococcus pneumoniae Rx1

46.087

100

0.466
  radA Streptococcus pneumoniae D39

46.087

100

0.466
  radA Streptococcus pneumoniae TIGR4

46.087

100

0.466
  radA Streptococcus pneumoniae R6

46.087

100

0.466
  radA Streptococcus mitis NCTC 12261

45.87

100

0.464
  radA Streptococcus mitis SK321

46.053

100

0.462

Multiple sequence alignment