Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AOY20_RS01495 Genome accession   NZ_CP012808
Coordinates   321427..322230 (-) Length   267 a.a.
NCBI ID   WP_081403332.1    Uniprot ID   -
Organism   Acinetobacter equi strain 114     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 316427..327230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY20_RS01475 (AOY20_01475) - 317482..318186 (-) 705 WP_054580229.1 YfgM family protein -
  AOY20_RS01480 (AOY20_01480) hisS 318217..319512 (-) 1296 WP_054580230.1 histidine--tRNA ligase -
  AOY20_RS01485 (AOY20_01485) ispG 319509..320624 (-) 1116 WP_054580231.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AOY20_RS01490 (AOY20_01490) - 320648..321436 (-) 789 WP_054580232.1 helix-turn-helix domain-containing protein -
  AOY20_RS01495 (AOY20_01495) pilF 321427..322230 (-) 804 WP_081403332.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AOY20_RS01500 (AOY20_01500) rlmN 322251..323486 (-) 1236 WP_054580233.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AOY20_RS01505 (AOY20_01505) ndk 323610..324041 (-) 432 WP_054580234.1 nucleoside-diphosphate kinase -
  AOY20_RS01510 (AOY20_01510) iscX 324153..324353 (-) 201 WP_054580235.1 Fe-S cluster assembly protein IscX -
  AOY20_RS01515 (AOY20_01515) - 324741..325946 (+) 1206 WP_054580236.1 RtcB family protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30446.83 Da        Isoelectric Point: 9.3931

>NTDB_id=157736 AOY20_RS01495 WP_081403332.1 321427..322230(-) (pilF) [Acinetobacter equi strain 114]
MSKPKLKGVCVFAILTVAFLMVGCQTVNGSKTDPKTAVQVRTQMAAEYIKQHRYDAAKRELDIALQLNSRDASANMMMGI
LLQQEGSQINIEKAERYLKTAIAAEPKNAQIRNNYGTYLSQIERYNEALQQFEIAGSTLGYEQRYTAFENKGRIYVKLND
IVNAEKSFNQALHVNRNSYVSMIELAELNYLRDDLPSATKFYQQAVALVGEKNLNARALWVGIRLARANGNPLEMQVLVN
QLRALYPDSQEYQRYLQLQYNTEVVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=157736 AOY20_RS01495 WP_081403332.1 321427..322230(-) (pilF) [Acinetobacter equi strain 114]
TTGAGTAAGCCCAAATTAAAAGGTGTATGTGTTTTTGCGATATTAACTGTTGCTTTTTTAATGGTTGGATGTCAAACCGT
AAATGGTTCTAAAACTGATCCAAAAACAGCTGTACAAGTGCGTACTCAAATGGCTGCCGAATATATTAAGCAGCATCGTT
ATGATGCTGCAAAACGTGAGCTAGATATTGCATTACAGTTGAATTCACGTGATGCTAGTGCAAATATGATGATGGGTATT
CTTCTTCAACAAGAAGGAAGTCAGATCAATATTGAAAAAGCAGAGCGTTATTTAAAAACAGCGATTGCTGCTGAGCCAAA
AAATGCTCAAATACGTAACAACTATGGTACGTATTTGTCTCAAATAGAACGTTATAATGAGGCTTTACAGCAATTTGAAA
TTGCAGGCTCTACATTGGGTTACGAACAGCGTTATACTGCCTTTGAAAATAAAGGCAGAATTTATGTGAAATTGAATGAT
ATAGTGAATGCCGAAAAATCATTTAATCAAGCATTGCATGTGAATCGTAATTCTTATGTTTCAATGATAGAGTTAGCAGA
ATTAAATTATCTACGTGATGATCTCCCATCTGCGACTAAGTTTTACCAACAAGCGGTAGCCTTAGTAGGGGAAAAAAATT
TAAATGCTCGTGCATTATGGGTTGGTATTCGATTAGCACGTGCAAATGGAAATCCATTGGAAATGCAGGTGCTTGTAAAT
CAGTTGCGAGCTTTGTACCCTGATAGCCAAGAATATCAACGTTATTTGCAATTACAGTACAATACTGAGGTCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(147-176)

Tetratricopeptide repeat (TPR_16)

(90-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

64.419

100

0.644
  pilF Acinetobacter baylyi ADP1

58.801

100

0.588

Multiple sequence alignment