Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LLUC063_RS11025 Genome accession   NZ_CP015905
Coordinates   2169247..2169693 (-) Length   148 a.a.
NCBI ID   WP_058225890.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain UC063     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2164247..2174693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUC063_RS10995 (LLUC063_2145) - 2164762..2165571 (-) 810 WP_014570791.1 metal ABC transporter permease -
  LLUC063_RS11000 (LLUC063_2146) - 2165564..2166301 (-) 738 WP_058221575.1 metal ABC transporter ATP-binding protein -
  LLUC063_RS11005 (LLUC063_2147) - 2166478..2167320 (-) 843 WP_003129990.1 metal ABC transporter substrate-binding protein -
  LLUC063_RS11010 (LLUC063_2148) - 2167317..2167754 (-) 438 WP_003129992.1 zinc-dependent MarR family transcriptional regulator -
  LLUC063_RS11015 (LLUC063_2149) - 2167961..2168908 (+) 948 WP_003130410.1 IS30 family transposase -
  LLUC063_RS11020 (LLUC063_2150) comGG 2168882..2169208 (-) 327 WP_058221568.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLUC063_RS11025 (LLUC063_2151) comGF 2169247..2169693 (-) 447 WP_058225890.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLUC063_RS11030 (LLUC063_2152) comGE 2169656..2169952 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLUC063_RS11035 (LLUC063_2153) comGD 2169924..2170355 (-) 432 WP_080585155.1 competence type IV pilus minor pilin ComGD Machinery gene
  LLUC063_RS11040 (LLUC063_2154) comGC 2170315..2170584 (-) 270 WP_003129998.1 competence type IV pilus major pilin ComGC Machinery gene
  LLUC063_RS11045 (LLUC063_2155) - 2170758..2171225 (-) 468 WP_058225891.1 hypothetical protein -
  LLUC063_RS11050 (LLUC063_2156) - 2171308..2171481 (-) 174 WP_160321615.1 hypothetical protein -
  LLUC063_RS11055 (LLUC063_2157) - 2171568..2172347 (-) 780 WP_058225892.1 peptidoglycan amidohydrolase family protein -
  LLUC063_RS11060 (LLUC063_2158) - 2172347..2172634 (-) 288 WP_023164507.1 phage holin -
  LLUC063_RS11065 (LLUC063_2159) - 2172647..2172997 (-) 351 WP_058225893.1 hypothetical protein -
  LLUC063_RS11070 (LLUC063_2160) - 2173010..2173246 (-) 237 WP_081196280.1 hypothetical protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17141.93 Da        Isoelectric Point: 8.6780

>NTDB_id=156099 LLUC063_RS11025 WP_058225890.1 2169247..2169693(-) (comGF) [Lactococcus lactis subsp. lactis strain UC063]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQSYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide


Download         Length: 447 bp        

>NTDB_id=156099 LLUC063_RS11025 WP_058225890.1 2169247..2169693(-) (comGF) [Lactococcus lactis subsp. lactis strain UC063]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGAGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(48-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

75.54

93.919

0.709
  comGF Streptococcus mutans UA140

47.518

95.27

0.453
  comGF Streptococcus mutans UA159

46.809

95.27

0.446
  comGF/cglF Streptococcus mitis SK321

42.336

92.568

0.392
  comGF/cglF Streptococcus mitis NCTC 12261

40.876

92.568

0.378
  comGF/cglF Streptococcus pneumoniae Rx1

39.716

95.27

0.378
  comGF/cglF Streptococcus pneumoniae D39

39.716

95.27

0.378
  comGF/cglF Streptococcus pneumoniae R6

39.716

95.27

0.378
  comGF/cglF Streptococcus pneumoniae TIGR4

39.716

95.27

0.378

Multiple sequence alignment