Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AM586_RS17700 Genome accession   NZ_CP012640
Coordinates   3960253..3961737 (+) Length   494 a.a.
NCBI ID   WP_047826910.1    Uniprot ID   A0A0J1D746
Organism   Massilia sp. WG5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3955253..3966737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM586_RS17675 (AM586_03515) - 3956678..3957700 (+) 1023 WP_047826914.1 SIS domain-containing protein -
  AM586_RS17680 (AM586_03520) - 3957700..3958233 (+) 534 WP_197416519.1 HAD family hydrolase -
  AM586_RS17685 (AM586_03525) lptC 3958247..3958855 (+) 609 WP_047826913.1 LPS export ABC transporter periplasmic protein LptC -
  AM586_RS17690 (AM586_03530) lptA 3958884..3959474 (+) 591 WP_047826912.1 lipopolysaccharide transport periplasmic protein LptA -
  AM586_RS17695 (AM586_03535) lptB 3959471..3960250 (+) 780 WP_229411295.1 LPS export ABC transporter ATP-binding protein -
  AM586_RS17700 (AM586_03540) rpoN/rpoN1 3960253..3961737 (+) 1485 WP_047826910.1 RNA polymerase factor sigma-54 Machinery gene
  AM586_RS17705 (AM586_03545) hpf 3961800..3962177 (+) 378 WP_047826909.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AM586_RS17710 (AM586_03550) - 3962277..3963362 (+) 1086 WP_047826908.1 enoyl-CoA hydratase/isomerase family protein -
  AM586_RS17715 (AM586_03555) - 3963440..3964357 (+) 918 WP_047826907.1 3-hydroxyacyl-CoA dehydrogenase family protein -
  AM586_RS17720 (AM586_03560) - 3964457..3965860 (+) 1404 WP_047826906.1 GntP family permease -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 54610.00 Da        Isoelectric Point: 4.9230

>NTDB_id=155942 AM586_RS17700 WP_047826910.1 3960253..3961737(+) (rpoN/rpoN1) [Massilia sp. WG5]
MKQTLQLKTSQHLALTPQLQQSIRLLQLSTLELHQEIEQVLSDNPLLERLDDPLDRSVRLLADGAINNGASTSTTPDAPP
AQSQGEEGKDNAGGEGEGGDSGEAGGAEHDMEWGEPGRSGNGGEDEDGRPQLEASPVSLREHLMEQVRLTCISPRDCALV
EIVVDTLDDNGYLEESLDELHARMPPELEIEFDELRTALSLVQSMDPPGVGARSAAECLALQIRRMPGVPMVTRRMALTI
VERYLTWFAQREFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAFASDVSDFVVPDVIVRRAKDGWSVQLNPEVMPRLRV
NSIYAALMKQGKSESQLNAQLQEAKWLIKNMRQRFDTILRVAQAIVERQRNFFSHGAVAMRPLVLREIADTLGLHESTIS
RVTTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIAEMLGEQGMVIARRTVAKYR
EALKIPPVSLRKSL

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=155942 AM586_RS17700 WP_047826910.1 3960253..3961737(+) (rpoN/rpoN1) [Massilia sp. WG5]
ATGAAACAGACGCTGCAACTCAAGACATCACAGCACCTTGCGCTGACGCCGCAACTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACTCTCGAGCTGCACCAGGAGATCGAGCAGGTACTGAGCGACAATCCGCTGCTCGAGCGCCTCGACGATCCGC
TCGACCGCTCCGTGCGCCTGCTGGCGGACGGCGCCATCAACAACGGCGCCTCGACCTCCACCACGCCTGACGCGCCGCCC
GCCCAGAGCCAGGGCGAGGAGGGCAAGGACAACGCCGGCGGCGAGGGCGAGGGCGGCGACAGCGGCGAGGCCGGCGGCGC
CGAGCACGACATGGAATGGGGCGAGCCGGGCCGCTCCGGCAACGGCGGCGAGGACGAAGACGGCCGCCCGCAGCTGGAAG
CCAGCCCGGTCTCGCTGCGTGAACACCTGATGGAACAGGTGCGCCTGACCTGCATCTCGCCGCGCGACTGCGCGCTGGTC
GAGATCGTCGTCGATACCCTCGACGACAACGGCTACCTCGAGGAATCGCTGGACGAGCTGCATGCGCGCATGCCGCCCGA
ACTGGAGATCGAGTTCGACGAACTGCGCACGGCCCTGTCGCTGGTGCAGAGCATGGACCCGCCCGGCGTCGGCGCCCGCA
GCGCCGCCGAATGCCTGGCGCTGCAGATCCGCCGCATGCCCGGCGTGCCGATGGTGACGCGGCGCATGGCGCTGACCATC
GTCGAACGCTACCTGACCTGGTTCGCGCAGCGCGAATTCAACAAGCTCAAGAAGGCCCTCGACTGCGACGACGAAGACCT
GCGCGAAGCCCAGGCCGTGATCCGCCAGTGCAATCCGCATCCGGGCGCGGCCTTCGCTTCCGACGTCTCCGACTTCGTGG
TGCCGGACGTGATCGTGCGCCGCGCGAAGGACGGCTGGAGCGTGCAGCTGAATCCGGAAGTGATGCCGCGCCTGCGCGTG
AACAGCATCTACGCTGCCTTGATGAAGCAGGGAAAATCGGAGTCGCAGCTGAATGCACAATTGCAAGAAGCGAAGTGGCT
CATCAAGAACATGCGCCAGCGCTTCGACACGATCCTGCGTGTAGCGCAAGCAATCGTCGAGCGCCAGCGGAACTTTTTCT
CCCACGGCGCAGTTGCCATGCGGCCCCTTGTTTTACGTGAGATAGCTGATACACTAGGTCTACACGAGAGCACAATTTCT
CGCGTCACAACGCAGAAATACATGCTGACGCCGCACGGCATGTTTGAGCTGAAGTACTTTTTCGGGAGTCACGTCGCGAC
CGAGGCAGGAGGCGAAGCATCCTCGACCGCGATCCGGGCTCTTATCGTACAACTCACAGGAGCAGAAGACCCCAAGAACC
CTTTATCCGACAGCAAGATCGCGGAGATGCTAGGGGAACAGGGAATGGTTATCGCACGCCGCACGGTCGCGAAATACCGT
GAGGCGCTGAAGATCCCGCCCGTTAGCCTTCGCAAATCCTTGTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-492)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-324)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

59.406

100

0.607

Multiple sequence alignment