Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AMQ28_RS05765 Genome accession   NZ_CP012608
Coordinates   1221363..1222109 (-) Length   248 a.a.
NCBI ID   WP_228137553.1    Uniprot ID   -
Organism   Acinetobacter sp. TTH0-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1216363..1227109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMQ28_RS05745 (AMQ28_05750) - 1217414..1218115 (-) 702 WP_053578780.1 YfgM family protein -
  AMQ28_RS05750 (AMQ28_05755) hisS 1218144..1219439 (-) 1296 WP_053578781.1 histidine--tRNA ligase -
  AMQ28_RS05755 (AMQ28_05760) ispG 1219447..1220562 (-) 1116 WP_053578782.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AMQ28_RS05760 (AMQ28_05765) - 1220587..1221372 (-) 786 WP_053578783.1 helix-turn-helix domain-containing protein -
  AMQ28_RS05765 (AMQ28_05770) pilF 1221363..1222109 (-) 747 WP_228137553.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AMQ28_RS05770 (AMQ28_05775) rlmN 1222186..1223421 (-) 1236 WP_053578785.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AMQ28_RS05775 (AMQ28_05780) ndk 1223544..1223975 (-) 432 WP_053578786.1 nucleoside-diphosphate kinase -
  AMQ28_RS05780 (AMQ28_05785) iscX 1224085..1224285 (-) 201 WP_053578787.1 Fe-S cluster assembly protein IscX -
  AMQ28_RS05785 (AMQ28_05790) - 1224376..1224927 (-) 552 WP_053578788.1 phosphatase PAP2 family protein -
  AMQ28_RS05790 (AMQ28_05795) - 1224958..1226241 (-) 1284 WP_053578789.1 glycosyltransferase family 4 protein -
  AMQ28_RS05795 (AMQ28_05800) - 1226432..1226848 (-) 417 WP_053578790.1 hypothetical protein -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 27981.70 Da        Isoelectric Point: 9.2475

>NTDB_id=155747 AMQ28_RS05765 WP_228137553.1 1221363..1222109(-) (pilF) [Acinetobacter sp. TTH0-4]
MAACQTQSTLGKKDPEKAIQVRTQLAAEYIKTGDLDSAKRALDQALEIESRDSAANMMMGILLQQEGSKVSMDKADAYFK
RAISADPKNAQARNNYGTYLYQTERYNEAIEQLNVAGTTLGYDQRFRALENVGRIYLKIGDVANAEKSFKQALQANRDSY
ISMLELSEIFYLKQQTAAATQLYEQFARGVGQKNQGARALWIGIRIARANSDKMGMQVLVNQLRALFPESPEYQRYLQLQ
YSTEAVWK

Nucleotide


Download         Length: 747 bp        

>NTDB_id=155747 AMQ28_RS05765 WP_228137553.1 1221363..1222109(-) (pilF) [Acinetobacter sp. TTH0-4]
ATGGCTGCTTGTCAAACTCAAAGTACATTAGGCAAAAAAGATCCTGAAAAAGCCATTCAGGTTCGAACTCAGTTGGCAGC
CGAATATATTAAAACGGGTGATTTAGATTCCGCAAAGCGGGCTTTAGATCAGGCACTCGAAATTGAGTCGAGAGATTCAG
CCGCAAATATGATGATGGGGATTTTACTGCAACAAGAAGGCAGTAAAGTAAGTATGGATAAAGCAGATGCTTATTTTAAG
CGTGCAATTTCTGCAGACCCTAAAAATGCACAAGCTCGTAACAACTATGGTACTTATTTGTATCAAACTGAACGCTATAA
TGAAGCGATTGAACAACTGAATGTTGCAGGTACAACATTAGGTTATGATCAGCGCTTTCGAGCTTTGGAAAATGTCGGTC
GGATTTATTTAAAAATAGGCGATGTTGCGAATGCTGAAAAATCATTCAAACAAGCTTTACAAGCCAATCGTGATTCTTAT
ATTTCAATGTTAGAGTTGTCAGAGATTTTTTATTTGAAACAACAGACCGCTGCTGCGACACAGCTGTATGAACAGTTTGC
GCGTGGGGTAGGTCAGAAAAATCAAGGTGCGCGGGCTCTTTGGATTGGTATCCGAATTGCACGGGCAAATAGTGACAAAA
TGGGTATGCAGGTACTTGTGAATCAATTACGTGCATTATTCCCAGAGAGTCCAGAATACCAACGTTATCTGCAATTACAG
TACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(21-50)

Tetratricopeptide repeat (TPR_19)

(73-128)

Tetratricopeptide repeat (TPR_16)

(133-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.757

99.597

0.794
  pilF Acinetobacter baylyi ADP1

71.774

100

0.718

Multiple sequence alignment