Detailed information NTG0155641

Bioinformatically predicted

Overview

• Name: amiA
• Type: Regulator
• Locus tag: AMD33_RS09515
• Genome accession: NZ_CP012588
• Coordinates: 1802564..1804531 (+)
• Length: 655 a.a.
• NCBI ID: WP_014607872.1
• Uniprot ID: Q8VTU5
• Organism: Streptococcus thermophilus strain MN-BM-A01
• Function: internalize XIP (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	1804569..1807959	1802564..1804531	flank	38

Gene organization within MGE regions

Location: 1802564..1807959

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AMD33_RS09515 (AMD33_09485)	amiA	1802564..1804531 (+)	1968	WP_014607872.1	peptide ABC transporter substrate-binding protein	Regulator
AMD33_RS09520 (AMD33_09490)	-	1804576..1805390 (-)	815	Protein_1790	ISL3 family transposase	-
AMD33_RS09525 (AMD33_09495)	-	1805638..1805964 (+)	327	WP_014607875.1	M20/M25/M40 family metallo-hydrolase	-
AMD33_RS09530 (AMD33_09500)	-	1806046..1807236 (+)	1191	WP_011680651.1	SAM-dependent methyltransferase	-
AMD33_RS09535 (AMD33_09505)	-	1807495..1807959 (-)	465	Protein_1793	transposase family protein	-

Sequence

Protein

• Length: 655 a.a.
• Molecular weight: 71730.84 Da
• Isoelectric Point: 5.0409

>NTDB_id=155641 AMD33_RS09515 WP_014607872.1 1802564..1804531(+) (amiA) [Streptococcus thermophilus strain MN-BM-A01]
MKLKKILGITGVALLSVGMLAACSSKSSTSGTTYSNIYGSDPETLDYITSIMGGTKAVLTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADAKSGALYLVQDSIAGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKQEPYWNSKTTYGVLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDSIKFSYSDGSDLESIERSFSDGALSIARVFPTSSNYASVEKKYKDNIFYTEPGASTSVIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQVNGKDGAALALRNLFVPYDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADGQEGLYNAEKAKTEFAKAKEALQGEGVQFPVHLDLPVDQSSKLSVAQAQSLKQTIEKSLGSENVVIDINQMSSDDMNN
VTSNAANAAAEDWDISNGVGWGPDYQDPSTYLDILKTTSSENTKIFNGYDDPNNAAAAQVGLKDYDALLDSAASETTDIN
ARYDRYAQAQAWLEDSSLIIPLTVSNGAAPVISRLTPFTGASIQVGDKGSSYVKYVKSQEKVVTKKEYEQSREKWLKERK
ASNEKAQKDLEKHVK

Nucleotide

• Length: 1968 bp

>NTDB_id=155641 AMD33_RS09515 WP_014607872.1 1802564..1804531(+) (amiA) [Streptococcus thermophilus strain MN-BM-A01]
ATGAAACTTAAAAAAATTCTTGGAATTACGGGTGTAGCCCTTCTAAGTGTTGGTATGTTAGCGGCTTGTTCTTCTAAGTC
AAGTACTAGTGGAACGACTTATAGTAATATTTATGGCTCAGATCCAGAAACCTTGGATTATATTACATCAATTATGGGAG
GGACAAAAGCTGTTTTGACTAATGGTGTAGATGGTCTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTGACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAGCATGCAGCTGATGCCAAATCTGGAGCCCTTT
ATTTGGTACAAGATTCTATTGCTGGATTGTCTGACTACCTTTCTGGTGCCAATAAAGATTTCTCAAATGTTGGCGTTAAA
GCAATAGACGATTACACTCTGCAATACACACTAAAAAAACAGGAACCATACTGGAACTCTAAGACAACCTATGGTGTGCT
CTTCCCAGTTAACGAAGACTTTCTTAAGAACAAAGGGAAAGATTTTGGTAAGTCAACAGACCCAACCTCTATCCTATATA
ACGGTCCTTTCCTTCTTAAATCTTTGACAGCTAAATCATCTATCGAGTTGACTAAGAATGAGAATTATTGGGATAAGAAA
AACGTGCATTTTGACTCTATCAAATTCTCATATTCTGATGGATCGGATCTAGAATCAATAGAACGTAGTTTCTCTGACGG
CGCTCTCAGCATTGCACGTGTCTTCCCTACGAGTTCAAACTATGCATCGGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGAACCAGGTGCATCTACATCGGTTATTGGTGTAAACATTGACCGCCAAAGCTATAAATTCTCAGCTAAGAAGACA
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTTCGTCAATCAATCAACTTCGCTATTGATCGTAC
AGCCTATCAATCACAAGTAAACGGTAAAGATGGCGCAGCTCTTGCTCTACGTAACCTTTTTGTTCCTTATGATTTTGTCA
GTGCAGGCGATAAGACCTTTGGTGATCTTGTGACAGAAAAAATGAGCTCTTATGGTGATGAGTGGTCTGGTGTTAACTTT
GCAGATGGTCAAGAGGGTCTCTACAATGCTGAAAAAGCTAAGACTGAGTTTGCCAAAGCCAAAGAGGCTCTTCAAGGAGA
AGGGGTACAATTCCCAGTTCACTTAGACCTTCCAGTTGATCAAAGTTCTAAACTTAGTGTTGCTCAAGCTCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAAATGTCATCTGATGACATGAATAAT
GTGACTAGTAATGCGGCAAATGCTGCAGCAGAAGACTGGGATATTTCTAACGGTGTGGGTTGGGGTCCAGACTATCAAGA
TCCATCAACTTATCTTGATATCTTAAAAACAACATCAAGTGAAAATACCAAGATTTTCAATGGTTATGATGATCCAAACA
ATGCAGCAGCAGCCCAAGTAGGTCTTAAGGATTATGATGCCTTGCTTGATTCAGCAGCTTCAGAAACAACAGACATCAAC
GCTCGTTACGACCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTATCATTCCATTGACTGTTAGTAACGG
TGCAGCACCAGTTATCTCACGTTTGACACCATTTACGGGTGCTAGCATACAGGTGGGTGATAAGGGCTCATCCTACGTCA
AATATGTGAAATCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAGGAAA
GCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACGTTAAATAA

Domains

Predicted by InterproScan.

• Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5) (75-520)

3D structure

• AlphaFold DB: Q8VTU5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiA	Streptococcus salivarius strain HSISS4	88.872	100	0.89
amiA3	Streptococcus thermophilus LMG 18311	86.454	100	0.867
amiA3	Streptococcus thermophilus LMD-9	86.454	100	0.867