Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AMS64_RS02830 Genome accession   NZ_CP012504
Coordinates   601122..602561 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain TH0426     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 596122..607561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMS64_RS02800 (AMS64_02795) mlaF 596699..597499 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  AMS64_RS02805 (AMS64_02800) - 597707..598702 (+) 996 WP_064335268.1 KpsF/GutQ family sugar-phosphate isomerase -
  AMS64_RS02810 (AMS64_02805) kdsC 598702..599256 (+) 555 WP_021231836.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AMS64_RS02815 (AMS64_02810) lptC 599253..599813 (+) 561 WP_021231837.1 LPS export ABC transporter periplasmic protein LptC -
  AMS64_RS02820 (AMS64_02815) lptA 599800..600327 (+) 528 WP_031227941.1 lipopolysaccharide transport periplasmic protein LptA -
  AMS64_RS02825 (AMS64_02820) lptB 600331..601056 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  AMS64_RS02830 (AMS64_02825) rpoN/rpoN1 601122..602561 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  AMS64_RS02835 (AMS64_02830) hpf 602582..602869 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  AMS64_RS02840 (AMS64_02835) ptsN 602872..603318 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AMS64_RS02845 (AMS64_02840) rapZ 603364..604230 (+) 867 WP_021231839.1 RNase adapter RapZ -
  AMS64_RS02850 (AMS64_02845) npr 604251..604523 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  AMS64_RS02855 (AMS64_02850) - 604541..606445 (-) 1905 WP_064335269.1 transcription antiterminator -
  AMS64_RS02860 (AMS64_02855) dagF 606552..607292 (-) 741 WP_064335270.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=154822 AMS64_RS02830 WP_005336331.1 601122..602561(+) (rpoN/rpoN1) [Aeromonas veronii strain TH0426]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=154822 AMS64_RS02830 WP_005336331.1 601122..602561(+) (rpoN/rpoN1) [Aeromonas veronii strain TH0426]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACTAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGACTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GACGAGTTGCCGGTGGATACCACCTGGGATGAGGTTTACTCTGCCGGCACGGCCCAAAGTGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACTACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCTTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATTCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGCATTGCTGCACGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCTT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGTCCCGGTAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCACGTAACGCCCGCAGCAGCACAGACACCCAGTTT
ATTCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTGGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCTCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491

Multiple sequence alignment