Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FORC36_RS13605 Genome accession   NZ_CP015512
Coordinates   2820673..2822397 (-) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain FORC_036     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2815673..2827397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC36_RS13570 (FORC36_2537) - 2816009..2816698 (-) 690 WP_085761353.1 PilW family protein -
  FORC36_RS13575 (FORC36_2538) - 2816698..2817264 (-) 567 WP_085761354.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC36_RS13580 (FORC36_2539) - 2817264..2817761 (-) 498 WP_085761355.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC36_RS13585 (FORC36_2540) - 2817817..2818287 (-) 471 WP_045622012.1 type II secretion system protein -
  FORC36_RS13590 (FORC36_2541) - 2818321..2818899 (-) 579 WP_085761356.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FORC36_RS13595 (FORC36_2542) - 2818978..2819439 (-) 462 WP_039554781.1 hypothetical protein -
  FORC36_RS13600 (FORC36_2543) - 2819443..2820663 (-) 1221 WP_011079398.1 type II secretion system F family protein -
  FORC36_RS13605 (FORC36_2544) pilF 2820673..2822397 (-) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  FORC36_RS13610 (FORC36_2545) - 2822394..2823515 (-) 1122 WP_085761357.1 tetratricopeptide repeat protein -
  FORC36_RS13615 (FORC36_2546) - 2823512..2824360 (-) 849 WP_085761358.1 ExeA family protein -
  FORC36_RS13620 (FORC36_2547) mshL 2824368..2826014 (-) 1647 WP_223370838.1 pilus (MSHA type) biogenesis protein MshL -
  FORC36_RS13625 (FORC36_2548) - 2826030..2826350 (-) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  FORC36_RS13630 (FORC36_2549) gspM 2826343..2826993 (-) 651 WP_045596391.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=154048 FORC36_RS13605 WP_011079397.1 2820673..2822397(-) (pilF) [Vibrio vulnificus strain FORC_036]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=154048 FORC36_RS13605 WP_011079397.1 2820673..2822397(-) (pilF) [Vibrio vulnificus strain FORC_036]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCTCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
TTACCAGAAGTGCATGCTCGTCGTTTACGCGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCTGCGGACCTTTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAATTGGTGGAAGGCTTTGACCGCTATTATCGCCGTACCAAAGAGATTGCCAACTTTGCCGAGCAGTTGCAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATTGAAGATGATGACAGTGATGAGGTGACGGTTGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATTCAGGTGGGGGCGTCGGATATCCATATCGAACCAGACGCCAATGTGTTGCGTTTGCGCCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTGAATGAAGTCAACATCGCGCCAGCATTGGTACTGAGATTGAAACTCATG
GCCAACTTGGATATTTCAGAAAAGCGTCTGCCACAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCATGGCATGATCCTTGTT
ACTGGCCCTACGGGTTCTGGTAAAACCACCACGCTGTATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACGGTGTTAAGAACGTTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGACATGGGGGCACCTGGTTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAGACAGAAGATCATGTTGGCGAGTCACGCAAAGCGTGGTTGGCACAACGCTTCCCAAATCAAGTG
GAAGCGGGTTTTGTGAAAGGGCGCGGTTGCCAAAACTGTAACTTAACAGGCTATCGCGGCCGTATTGGTGTGTTTGAAAT
GCTGGAACTTGAACATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCGCATGCTGCGCGTCGTTCGC
CAGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGTTGGCGCTACAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371