Detailed information NTG0153948

Bioinformatically predicted

Overview

• Name: pilJ
• Type: Machinery gene
• Locus tag: amyaer_RS16425
• Genome accession: NZ_CP012375
• Coordinates: 3333232..3335895 (+)
• Length: 887 a.a.
• NCBI ID: WP_066030049.1
• Uniprot ID: -
• Organism: Microcystis aeruginosa NIES-2481
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	3331339..3332562	3333232..3335895	flank	670

Gene organization within MGE regions

Location: 3331339..3335895

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
amyaer_RS16415 (amyaer_3414)	-	3331339..3332562 (+)	1224	WP_046662876.1	RNA-guided endonuclease TnpB family protein	-
amyaer_RS16420 (amyaer_3415)	-	3332608..3333138 (+)	531	WP_066030048.1	chemotaxis protein CheW	-
amyaer_RS16425 (amyaer_3416)	pilJ	3333232..3335895 (+)	2664	WP_066030049.1	HAMP domain-containing methyl-accepting chemotaxis protein	Machinery gene

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 96921.97 Da
• Isoelectric Point: 4.3785

>NTDB_id=153948 amyaer_RS16425 WP_066030049.1 3333232..3335895(+) (pilJ) [Microcystis aeruginosa NIES-2481]
MPSGKEYAKLYGKANTAYCQGNLSDAAVIVKDMISKYPDDANVILLQGHIYLGLEQYSLAEKRYEKVLELAKNSPNRFDL
VDYARRGLDQIRQLRFEPEDGDFTIAVTESIAYSEAAFDPSQGFSNSLSGRSQGADPDLQNESIDWNSGLFNEEYLGEPT
VGTVDSYGDDFGESEETAFGSLPLASSDSHGSQWSGDSPDGELPFPFLEAAEIDSLPEGWGGGLGSRGEATFMASSDWRE
NPLTRRDQELSYPDTLETNTFAIDPNPTYRQAAKTGLNEGIVTQKRYEEDQTGDFRELKLNDEDLDGFSPLHLTDIAEEL
GESELFTRSGEEALTAGFVPEPSARLHASSAVGESSFNPSRLEIPDDKRDSHLGPVSERLLKPVVEVNPGKLAFFVNASL
GKKQLILAALAGITPVVLIFAVSTTSWLFSLSAAKPGPNSKEKTEKVAPKPAPASLSLFSQPVLTMMLLTGLGTLGLTWL
GLQLLISQIKRSLNDLQSQFDHLSEGNFNAKATIYSEDEFGQLANRFNQMYRVVLTTTTEAQRRAAETEQEREDLQRQVI
RLLDDVEGAARGDLTVEAEVSADVLGAVADAFNLTIQNLREIVRQVKKAAKQVNQASTNSESFARNQSSDALRMAEELAV
TLNSVQMMTDSIQRVAENAREAEEVARTSSITALKGGDSVERTVAGILQIRDTVSETARKVKRLAEASQEISKIVAVVSQ
IASRTNLLALNASIQAARAGDAGRGFAVVADEVRQLADRAAKSLKEIEQIVLQIQSETGSVMTAMEEGIQQVIDVTERSE
QAKKSLEDIIQVSNRIDTLVRSITADTVKQRENSLNVAQVMQSVELTAQETSQESQRVAGSLQKLVSISRELLSSVERFK
VDSEDDR

Nucleotide

• Length: 2664 bp

>NTDB_id=153948 amyaer_RS16425 WP_066030049.1 3333232..3335895(+) (pilJ) [Microcystis aeruginosa NIES-2481]
ATGCCGTCAGGGAAAGAATACGCGAAACTCTATGGAAAGGCTAACACTGCCTATTGCCAGGGCAACCTCTCAGATGCCGC
CGTCATTGTCAAAGACATGATCAGCAAGTATCCCGATGATGCCAATGTCATCCTTTTACAGGGACACATTTATCTAGGAC
TAGAACAATACAGCTTGGCAGAGAAACGTTACGAAAAAGTGCTGGAATTGGCTAAAAATTCTCCCAATCGCTTTGATCTG
GTGGATTATGCTCGACGGGGTCTCGATCAGATTCGGCAATTGCGTTTTGAACCCGAGGATGGAGATTTTACGATCGCTGT
TACAGAAAGCATTGCCTACAGTGAGGCAGCTTTCGATCCCAGTCAAGGTTTCAGCAATTCTTTGTCTGGGCGGTCGCAAG
GGGCTGATCCTGATCTGCAAAATGAGTCAATAGATTGGAATAGTGGTCTTTTCAATGAGGAGTATCTCGGGGAGCCTACG
GTGGGTACAGTCGATTCCTATGGCGATGATTTTGGGGAATCGGAGGAAACCGCCTTTGGTTCCCTTCCCCTGGCCAGTTC
AGACTCTCACGGTTCCCAGTGGTCTGGCGATTCTCCAGATGGAGAACTCCCTTTCCCCTTTTTAGAAGCAGCGGAGATTG
ACTCGCTGCCGGAAGGGTGGGGTGGTGGGTTAGGGTCAAGGGGAGAAGCCACTTTTATGGCTTCCTCTGACTGGCGGGAA
AATCCCCTCACTCGGAGAGATCAGGAGTTATCCTATCCCGATACCCTAGAAACAAACACTTTTGCCATTGACCCGAACCC
GACCTACCGACAAGCGGCGAAAACTGGCCTTAATGAGGGCATAGTCACTCAAAAAAGATACGAGGAGGACCAAACCGGAG
ATTTTAGAGAACTAAAACTCAATGATGAGGATTTAGACGGTTTTTCCCCACTGCATCTGACGGATATAGCCGAAGAACTG
GGGGAATCGGAGCTATTTACCCGTTCGGGAGAGGAAGCTTTAACTGCTGGTTTTGTCCCCGAACCCAGTGCGCGCCTTCA
TGCCAGTTCTGCTGTGGGAGAGTCTAGTTTTAATCCTTCTCGTCTAGAAATCCCCGATGACAAAAGAGATAGTCATCTTG
GCCCTGTTTCCGAGCGCCTGCTCAAGCCAGTGGTGGAAGTCAATCCGGGCAAATTAGCCTTTTTTGTCAATGCTTCTTTG
GGCAAAAAACAACTAATCCTAGCGGCTTTAGCGGGTATTACCCCTGTAGTCTTGATTTTTGCCGTTAGTACCACCTCTTG
GCTATTTTCTCTATCGGCTGCCAAACCGGGCCCGAATAGCAAGGAAAAAACCGAAAAAGTTGCCCCGAAACCGGCCCCAG
CCAGCCTTTCCCTTTTTAGTCAACCGGTACTAACCATGATGTTATTAACCGGACTGGGAACCCTTGGCCTGACTTGGCTA
GGTTTACAGTTGCTAATTAGTCAAATTAAGCGCAGTCTCAACGATCTGCAAAGCCAATTCGATCATCTCTCGGAAGGCAA
TTTTAACGCTAAAGCGACGATCTACTCGGAAGATGAATTCGGTCAACTGGCTAATCGTTTCAATCAGATGTACCGGGTGG
TGCTAACTACCACCACGGAAGCCCAACGCCGCGCCGCCGAAACGGAACAGGAACGGGAAGATCTACAAAGGCAAGTGATT
CGACTTCTCGATGATGTGGAAGGAGCGGCTCGGGGGGATCTGACCGTGGAGGCGGAGGTAAGTGCCGATGTGTTGGGGGC
GGTGGCCGATGCTTTTAATTTGACTATCCAAAACCTGCGCGAAATTGTCCGACAGGTGAAAAAAGCGGCAAAACAGGTGA
ATCAAGCCAGTACCAATAGTGAATCTTTCGCCCGCAATCAGTCTAGCGATGCCCTGCGGATGGCGGAGGAATTAGCAGTC
ACCCTTAATTCGGTACAGATGATGACCGACTCAATTCAACGGGTGGCAGAAAACGCCAGAGAAGCCGAAGAAGTCGCCCG
CACTTCCTCGATTACCGCCCTGAAGGGGGGCGATTCCGTGGAAAGAACCGTGGCGGGAATTCTGCAAATCCGCGACACTG
TATCAGAAACGGCGCGAAAAGTGAAGCGTTTGGCGGAAGCTTCCCAGGAAATCTCGAAAATTGTCGCAGTAGTCTCTCAG
ATTGCCTCGCGGACCAATTTATTGGCTCTTAATGCCTCGATTCAAGCAGCGCGAGCAGGAGATGCGGGCCGCGGTTTTGC
AGTGGTGGCCGATGAGGTGCGTCAGTTGGCCGATAGAGCCGCTAAATCTCTTAAGGAAATTGAACAGATCGTTTTACAGA
TTCAATCGGAAACGGGTTCGGTAATGACGGCAATGGAGGAAGGGATTCAACAGGTTATCGATGTCACCGAGCGCTCGGAA
CAGGCCAAGAAGTCCCTAGAAGATATTATCCAAGTGTCTAACCGCATCGATACGCTGGTGCGCTCGATTACCGCCGATAC
GGTCAAACAACGGGAGAATTCTCTCAATGTCGCCCAAGTTATGCAGTCGGTGGAGTTAACCGCTCAGGAAACTTCCCAAG
AATCCCAACGGGTGGCCGGTTCTCTGCAAAAATTGGTTAGTATCTCCCGGGAGCTGCTGTCTTCGGTAGAACGGTTTAAG
GTAGACTCAGAGGATGATCGATAA

Domains

Predicted by InterproScan.

• Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal) (664-844)

• HAMP domain (HAMP) (484-531)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilJ	Synechocystis sp. PCC 6803	50.217	100	0.521