Detailed information NTG0153933

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: A4W82_RS03715
• Genome accession: NZ_CP015487
• Coordinates: 728381..730798 (+)
• Length: 805 a.a.
• NCBI ID: WP_076647394.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain TMW 1.46
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 723381..735798

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4W82_RS03685 (A4W82_03650)	recN	723695..725398 (+)	1704	WP_129584120.1	DNA repair protein RecN	Machinery gene
A4W82_RS03690 (A4W82_03655)	-	725457..725780 (-)	324	WP_025015871.1	hypothetical protein	-
A4W82_RS03695 (A4W82_03660)	-	725900..726127 (+)	228	WP_197703335.1	YdbC family protein	-
A4W82_RS03700 (A4W82_03665)	gmk	726200..726811 (+)	612	WP_011374394.1	guanylate kinase	-
A4W82_RS03705 (A4W82_03670)	rpoZ	726816..727073 (+)	258	WP_011374395.1	DNA-directed RNA polymerase subunit omega	-
A4W82_RS03710 (A4W82_03675)	coaBC	727162..728379 (+)	1218	WP_016264860.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
A4W82_RS03715 (A4W82_03680)	priA	728381..730798 (+)	2418	WP_076647394.1	primosomal protein N'	Machinery gene
A4W82_RS03720 (A4W82_03685)	fmt	730818..731774 (+)	957	WP_076647396.1	methionyl-tRNA formyltransferase	-
A4W82_RS03725 (A4W82_03690)	rsmB	731764..733107 (+)	1344	WP_251925853.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
A4W82_RS03730 (A4W82_03695)	-	733129..733875 (+)	747	WP_025015868.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
A4W82_RS03735 (A4W82_03700)	pknB	733872..735755 (+)	1884	WP_025015867.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 90583.08 Da
• Isoelectric Point: 7.8594

>NTDB_id=153933 A4W82_RS03715 WP_076647394.1 728381..730798(+) (priA) [Latilactobacillus sakei strain TMW 1.46]
MPQIAKVIVDVPTMQTNRPYDYQVPVTLVDVIQPGMRVIIPFGNGDRKIQGFVLQVLDQPSFTGQLKAIEAVVDLTPAVN
PELLSLSDWLAQQTYAFQITCLQTMLPSVMRAKYTKTATLTDEIPTDLQASLFNGNDELTFDQNLAPDKLKQLMQLQRQG
KISVTYHVDNRAQVKQLTAIEPALSFEQLEEARMGLRKSAQKQSQLISYLQTLIDQEAPTLLKTILEKTTFTRENIRIGA
EKGWLRQVKVEAYRNPYANAVQPTHKMTLTSEQETAVDAVVQSASTDASEVFLIEGVTGSGKTEVYLQTIDAVLQQGKTA
LMLVPEIALTPQMVQRVKGRFGDLVAVLHSGLSEGEKYDEWRRIERKEARVVVGARSAVFAPLENIGVIIMDEEHEGSYK
QDESPRYHARDVAIWRSQYHHCPVILGSATPSLESRARAQKDVYHLIRLTQRVNQQALPAVELVDMREAMKVQKASNFSP
ALLAGIKERMQKGEQSVLLLNRRGFSSFIMCRDCGFVLQCPNCDISLTLHMDTHSMKCHYCGHEEAIPKICPSCQSRQIR
YFGTGTQKVEAELQELIPEARILRMDVDTTRKKGAHAKLLKKFGDHEADILLGTQMIAKGLDFPDVTLVGVINADTALGL
PDFRASERTFQLLTQVSGRAGRADKVGQVIVQSYNPENYAIQFAKQHDYEGFYQYEMAVRHRGGYPPYYFTVKVTVSHPE
EALAAKKSFQIAKQMRPILSDQALILGPTPKSIARINNRYFYQIVIKYKQEPQLQAKLAEILETTQIDTRHGFKIGLDNE
PLQFT

Nucleotide

• Length: 2418 bp

>NTDB_id=153933 A4W82_RS03715 WP_076647394.1 728381..730798(+) (priA) [Latilactobacillus sakei strain TMW 1.46]
ATGCCACAGATTGCTAAAGTAATCGTTGATGTGCCGACAATGCAAACTAATCGGCCATATGATTATCAGGTACCGGTCAC
TTTAGTTGATGTCATTCAACCTGGGATGCGGGTTATTATTCCGTTTGGAAATGGTGATCGTAAGATTCAGGGCTTTGTCT
TGCAAGTTTTAGACCAGCCAAGCTTTACGGGCCAATTAAAGGCAATCGAGGCAGTTGTTGATTTAACGCCAGCGGTTAAT
CCTGAATTACTATCACTTTCGGATTGGTTAGCACAACAGACGTATGCTTTTCAGATCACGTGTCTGCAGACGATGTTACC
CAGTGTGATGCGGGCTAAATATACGAAAACTGCGACGTTAACTGATGAAATTCCAACGGACTTACAGGCCAGTCTGTTCA
ATGGCAATGACGAGTTGACTTTTGATCAGAATCTGGCGCCTGATAAGTTAAAACAACTCATGCAATTGCAACGCCAAGGT
AAAATTAGCGTCACTTATCACGTTGACAACCGGGCACAAGTTAAGCAATTAACGGCGATTGAGCCGGCACTGAGTTTTGA
ACAATTGGAAGAAGCACGGATGGGCTTGCGAAAATCAGCCCAGAAGCAAAGCCAATTAATCAGTTATCTGCAAACGCTGA
TTGATCAAGAGGCACCGACTTTATTAAAAACGATTTTAGAAAAAACAACTTTTACGCGGGAAAATATTCGAATCGGTGCG
GAAAAAGGCTGGTTACGCCAAGTTAAGGTGGAAGCTTACCGGAATCCGTACGCTAATGCTGTTCAACCAACCCACAAAAT
GACGCTAACGAGCGAACAAGAAACCGCAGTTGATGCGGTCGTTCAATCCGCATCAACGGATGCCTCAGAAGTATTCTTAA
TTGAAGGTGTCACCGGCAGTGGGAAGACCGAAGTTTATTTACAAACAATTGATGCGGTCTTGCAACAAGGGAAGACCGCA
CTGATGTTAGTCCCTGAAATTGCTTTGACGCCGCAGATGGTGCAACGCGTGAAGGGCCGCTTTGGTGATTTAGTTGCTGT
GTTACATAGTGGCTTGTCTGAAGGTGAGAAATACGACGAGTGGCGGCGCATTGAACGCAAAGAAGCGCGCGTGGTCGTCG
GTGCCCGCTCAGCCGTTTTTGCGCCGCTTGAAAATATCGGCGTGATTATTATGGATGAAGAACATGAAGGCAGTTATAAA
CAAGACGAAAGTCCCCGTTATCATGCACGCGACGTTGCGATTTGGCGGAGTCAGTATCATCACTGCCCGGTCATTTTAGG
CAGCGCCACACCATCGTTAGAATCCCGCGCTCGGGCGCAAAAAGATGTCTACCATCTGATTCGCCTGACGCAACGGGTGA
ACCAACAAGCCTTACCAGCTGTTGAACTAGTTGATATGCGCGAAGCGATGAAGGTTCAAAAAGCCAGCAACTTTTCACCG
GCGTTATTAGCCGGCATCAAAGAACGGATGCAAAAGGGCGAACAAAGTGTGTTACTCCTAAATCGTCGCGGTTTTTCATC
ATTTATCATGTGTCGGGATTGCGGGTTTGTCTTGCAGTGTCCCAACTGTGATATTTCGTTGACGTTACACATGGATACGC
ATTCGATGAAATGCCATTATTGTGGGCACGAAGAAGCGATTCCTAAGATTTGCCCCAGCTGTCAAAGCCGCCAAATTCGC
TATTTTGGGACTGGCACGCAAAAAGTCGAAGCTGAATTACAAGAATTAATCCCTGAAGCACGGATCTTGCGGATGGACGT
TGATACGACTCGCAAAAAAGGCGCGCATGCTAAATTATTGAAAAAATTTGGTGATCACGAAGCTGATATCTTATTAGGAA
CGCAGATGATTGCCAAGGGCCTAGATTTTCCAGATGTAACGTTGGTCGGGGTGATCAACGCTGATACAGCTTTGGGGTTA
CCGGATTTTCGGGCGAGTGAGCGGACTTTCCAATTACTAACGCAGGTTAGTGGTCGTGCGGGTCGTGCGGATAAGGTGGG
GCAAGTGATTGTTCAATCTTATAATCCCGAGAATTACGCGATTCAATTTGCTAAACAGCACGATTATGAAGGCTTTTATC
AATATGAAATGGCGGTGCGCCACCGTGGCGGCTATCCGCCTTATTACTTTACGGTGAAGGTGACGGTTAGCCACCCTGAA
GAAGCACTTGCCGCAAAGAAGAGTTTTCAAATTGCCAAACAGATGCGGCCGATTTTATCCGACCAAGCACTGATTCTCGG
GCCAACCCCGAAATCAATTGCGCGGATTAATAATCGTTATTTTTACCAAATTGTGATTAAATATAAACAGGAACCACAAC
TTCAGGCTAAATTAGCCGAAATACTAGAGACCACCCAAATCGATACTCGTCACGGGTTTAAGATTGGGCTGGATAATGAA
CCTCTACAATTTACTTAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (266-432)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (520-545)

• Helicase conserved C-terminal domain (Helicase_C) (561-663)

• Primosomal protein N C-terminal domain (PriA_C) (707-801)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-107)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	51.792	100	0.52