Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   F822_RS07635 Genome accession   NZ_CP012371
Coordinates   1691818..1693287 (-) Length   489 a.a.
NCBI ID   WP_025041449.1    Uniprot ID   -
Organism   Nitrosospira briensis C-128     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1686818..1698287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F822_RS07610 mutY 1687660..1688742 (+) 1083 WP_025041444.1 A/G-specific adenine glycosylase -
  F822_RS07615 - 1688788..1689411 (+) 624 WP_025041445.1 5-formyltetrahydrofolate cyclo-ligase -
  F822_RS07620 rapZ 1689530..1690369 (-) 840 WP_025041446.1 RNase adapter RapZ -
  F822_RS07625 hprK 1690413..1691381 (-) 969 WP_025041447.1 HPr(Ser) kinase/phosphatase -
  F822_RS07630 hpf 1691466..1691798 (-) 333 WP_025041448.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  F822_RS07635 rpoN/rpoN1 1691818..1693287 (-) 1470 WP_025041449.1 RNA polymerase factor sigma-54 Machinery gene
  F822_RS07640 lptB 1693337..1694059 (-) 723 WP_025041450.1 LPS export ABC transporter ATP-binding protein -
  F822_RS07645 lptA 1694087..1694701 (-) 615 WP_231623398.1 lipopolysaccharide transport periplasmic protein LptA -
  F822_RS07650 lptC 1694685..1695257 (-) 573 WP_025041452.1 LPS export ABC transporter periplasmic protein LptC -
  F822_RS07655 - 1695254..1695778 (-) 525 WP_025041453.1 HAD family hydrolase -
  F822_RS07660 ispH 1696318..1697262 (-) 945 WP_025041454.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55361.05 Da        Isoelectric Point: 5.0968

>NTDB_id=153887 F822_RS07635 WP_025041449.1 1691818..1693287(-) (rpoN/rpoN1) [Nitrosospira briensis C-128]
MKPTLQFKLSQHLTLTPQLQQSIRLLQLSTAELSQEIERMVQENPLLELDDHIGSDPIDYRNMPSDIRAEPLSDLIETPD
EASAKLLSIEDSSDTRKMTDESDWFSDDRAVRNTRDDDDERDFQQQAAEPPSLREHLNLQLSLSQIPERGRRIVGLLIDS
LDDDGYLAQDLEELVELLPPELAIDIDDLHNALEQLQRMDPPGIGARNLRECLLMQLHALPECTPYLDQAVLLVNDDLES
LASRDFATIKKLLHCDDECLRSIRHLITRLNPRPGMAFSSTVARYIIPDVIVTKISGSWVASLNPEAMPRLKINRLYADI
LKRSHDDSARGLSGHLNEAKWLIKNVHQRFSTILKVSSAVVERQQQFFEHGAVAMRPMVLREIADTLDLHESTISRVTTQ
KFMRTPRGIFELKYFFGSHVATDTGGACSATAIRALIKQMINAENAQKPLSDSQISEILGQQGIMVARRTVAKYRESIQI
PPVNLRKSF

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=153887 F822_RS07635 WP_025041449.1 1691818..1693287(-) (rpoN/rpoN1) [Nitrosospira briensis C-128]
ATGAAGCCTACTCTCCAGTTTAAGCTGTCGCAGCACTTGACGCTTACTCCGCAATTGCAACAATCCATACGGCTGCTACA
GCTTTCCACGGCCGAACTGAGCCAGGAAATAGAACGTATGGTTCAAGAAAATCCTCTCCTTGAACTTGACGATCATATCG
GTAGCGATCCGATCGACTATCGGAATATGCCCAGCGATATTCGGGCAGAGCCGTTATCGGATCTGATTGAGACTCCGGAC
GAAGCCAGTGCGAAATTGCTTTCTATCGAGGACAGCTCCGACACGAGAAAGATGACCGATGAGTCAGACTGGTTCTCGGA
CGATAGAGCCGTTCGAAACACGCGGGATGACGATGACGAACGGGATTTTCAGCAACAAGCGGCGGAACCGCCCAGCTTGC
GTGAACATCTTAACCTGCAGCTAAGCCTTAGCCAGATTCCAGAGCGTGGGCGAAGAATCGTTGGTTTGCTGATCGACAGC
CTGGATGACGACGGCTATCTTGCACAGGATCTTGAAGAGCTCGTTGAACTGCTGCCGCCCGAGCTCGCGATAGACATCGA
CGATCTTCACAATGCTCTGGAACAGTTGCAACGCATGGATCCTCCCGGCATTGGGGCGCGTAACCTCAGGGAATGCCTGC
TCATGCAATTGCATGCGCTGCCGGAATGTACTCCTTACCTGGATCAGGCGGTGCTGCTGGTGAACGACGATCTTGAAAGC
CTGGCATCACGGGATTTCGCCACCATTAAAAAACTGCTGCACTGTGATGATGAATGCCTGCGATCGATCCGGCACCTGAT
TACCCGACTGAACCCGAGACCCGGCATGGCATTCAGTTCCACTGTCGCCCGTTACATCATACCCGACGTAATTGTGACAA
AGATCAGCGGTTCATGGGTAGCCAGCCTTAATCCTGAAGCAATGCCCCGCCTCAAAATCAACCGGCTCTATGCTGACATC
CTGAAACGCTCTCATGATGATTCCGCACGAGGCCTATCGGGCCATCTGAATGAGGCGAAATGGCTCATCAAGAACGTACA
TCAGCGTTTCAGCACGATACTCAAGGTATCCTCAGCAGTGGTCGAACGCCAGCAACAGTTTTTCGAGCATGGCGCAGTGG
CCATGCGGCCCATGGTATTGCGGGAAATTGCGGATACGCTTGACTTGCATGAATCCACGATTTCGCGGGTAACGACACAA
AAATTCATGAGGACGCCACGGGGCATATTTGAATTAAAATATTTCTTTGGCAGCCATGTAGCGACCGATACCGGCGGCGC
TTGTTCCGCCACCGCAATTCGTGCTCTGATCAAGCAAATGATAAACGCCGAAAATGCACAGAAGCCACTGAGCGATAGCC
AGATTTCAGAAATTCTGGGGCAGCAGGGAATTATGGTTGCCCGCCGTACCGTGGCAAAATATCGGGAGTCCATACAAATT
CCTCCCGTTAATCTTCGGAAATCATTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(123-317)

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-487)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.767

100

0.546

Multiple sequence alignment