Detailed information NTG0153559

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: AS891_RS00360
• Genome accession: NZ_CP015375
• Coordinates: 90945..92066 (-)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis subsp. subtilis strain KCTC 3135
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 85945..97066

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AS891_RS00340 (AS891_00340)	znuC	86454..87149 (-)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-
AS891_RS00345 (AS891_00345)	znuA	87205..88164 (-)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
AS891_RS00350 (AS891_00350)	ycdG	88352..90037 (-)	1686	WP_003234736.1	glycoside hydrolase family 13 protein	-
AS891_RS00355 (AS891_00355)	ycdF	90062..90838 (-)	777	WP_003246415.1	SDR family oxidoreductase	-
AS891_RS00360 (AS891_00360)	rapF	90945..92066 (-)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
AS891_RS00365 (AS891_00365)	cwlK	92189..92692 (+)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
AS891_RS00370 (AS891_00370)	ycdC	92700..94061 (-)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
AS891_RS00375 (AS891_00375)	ycdB	94248..95666 (-)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
AS891_RS22700	-	95840..95971 (+)	132	WP_003246334.1	hypothetical protein	-
AS891_RS00380 (AS891_00380)	ycdA	95994..97058 (+)	1065	WP_003234750.1	DUF4352 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=153559 AS891_RS00360 WP_003246478.1 90945..92066(-) (rapF) [Bacillus subtilis subsp. subtilis strain KCTC 3135]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=153559 AS891_RS00360 WP_003246478.1 90945..92066(-) (rapF) [Bacillus subtilis subsp. subtilis strain KCTC 3135]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405