Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I51_RS00540 Genome accession   NZ_CP015244
Coordinates   117395..117835 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli O91 str. RM7190     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112395..122835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I51_RS00520 (I51_00520) coaE 112885..113505 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  I51_RS26545 - 113530..113574 (+) 45 WP_120795372.1 protein YacM -
  I51_RS00525 (I51_00525) guaC 113730..114773 (+) 1044 WP_001217338.1 GMP reductase -
  I51_RS00530 (I51_00530) hofC 114808..116010 (-) 1203 WP_000157234.1 protein transport protein HofC -
  I51_RS00535 (I51_00535) pilB 116000..117385 (-) 1386 WP_001025149.1 type II secretion system protein GspE Machinery gene
  I51_RS00540 (I51_00540) pilA 117395..117835 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  I51_RS00550 (I51_00550) nadC 118038..118931 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I51_RS00555 (I51_00555) ampD 119019..119570 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I51_RS00560 (I51_00560) ampE 119567..120421 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  I51_RS00565 (I51_00565) aroP 120464..121837 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=152902 I51_RS00540 WP_000360904.1 117395..117835(-) (pilA) [Escherichia coli O91 str. RM7190]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=152902 I51_RS00540 WP_000360904.1 117395..117835(-) (pilA) [Escherichia coli O91 str. RM7190]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363