Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   A5955_RS09875 Genome accession   NZ_CP015241
Coordinates   1988568..1989236 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 2013C-4465     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1983568..1994236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A5955_RS09845 (A5955_09840) rfaQ 1983746..1984804 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  A5955_RS09850 (A5955_09845) waaA 1985217..1986494 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  A5955_RS09855 (A5955_09850) coaD 1986502..1986981 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  A5955_RS09860 (A5955_09855) mutM 1987020..1987829 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  A5955_RS09865 (A5955_09860) rpmG 1987927..1988094 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  A5955_RS09870 (A5955_09865) rpmB 1988115..1988351 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  A5955_RS09875 (A5955_09870) radC 1988568..1989236 (-) 669 WP_001297375.1 RadC family protein Machinery gene
  A5955_RS09880 (A5955_09875) coaBC 1989408..1990628 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  A5955_RS09885 (A5955_09880) dut 1990606..1991064 (+) 459 WP_000976070.1 dUTP diphosphatase -
  A5955_RS09890 (A5955_09885) slmA 1991171..1991767 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  A5955_RS09895 (A5955_09890) pyrE 1991804..1992445 (-) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  A5955_RS09900 (A5955_09895) rph 1992511..1993227 (-) 717 WP_001247093.1 ribonuclease PH -
  A5955_RS09905 (A5955_09900) yicC 1993354..1994217 (+) 864 WP_000621339.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=152853 A5955_RS09875 WP_001297375.1 1988568..1989236(-) (radC) [Escherichia coli strain 2013C-4465]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=152853 A5955_RS09875 WP_001297375.1 1988568..1989236(-) (radC) [Escherichia coli strain 2013C-4465]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496