Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   AEI22_RS05185 Genome accession   NZ_CP012220
Coordinates   989478..990428 (+) Length   316 a.a.
NCBI ID   WP_115794555.1    Uniprot ID   -
Organism   Campylobacter jejuni strain CJ090CC1332     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 971733..1003427 989478..990428 within 0


Gene organization within MGE regions


Location: 971733..1003427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AEI22_RS05100 (AEI22_05090) pglI 971733..972662 (-) 930 WP_115794544.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  AEI22_RS05105 (AEI22_05095) pglH 972655..973734 (-) 1080 WP_115794545.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  AEI22_RS05110 (AEI22_05100) pglK 973731..975425 (-) 1695 WP_115794546.1 ABC-type lipopolysaccharide transporter PglK -
  AEI22_RS05115 (AEI22_05105) galE 975419..976405 (-) 987 WP_002874249.1 UDP-glucose 4-epimerase GalE -
  AEI22_RS05120 (AEI22_05110) - 976463..977257 (-) 795 WP_115794547.1 3'-5' exonuclease -
  AEI22_RS05125 (AEI22_05115) waaC 977323..978351 (+) 1029 WP_052781720.1 lipopolysaccharide heptosyltransferase I -
  AEI22_RS05130 (AEI22_05120) htrB 978341..979228 (+) 888 WP_002937339.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  AEI22_RS05135 (AEI22_05125) - 979225..980772 (+) 1548 WP_115794548.1 glycosyltransferase -
  AEI22_RS05140 (AEI22_05130) - 980776..981960 (+) 1185 WP_115794549.1 glycosyltransferase family 2 protein -
  AEI22_RS05145 (AEI22_05135) - 982017..983060 (+) 1044 WP_058000726.1 beta-1,4-N-acetylgalactosaminyltransferase -
  AEI22_RS05150 (AEI22_05140) - 983046..983951 (-) 906 WP_058000727.1 glycosyltransferase family 2 protein -
  AEI22_RS05155 (AEI22_05145) - 984040..984915 (+) 876 WP_115794550.1 alpha-2,3-sialyltransferase -
  AEI22_RS05160 (AEI22_05150) neuB 984916..985956 (+) 1041 WP_115794551.1 N-acetylneuraminate synthase -
  AEI22_RS05165 (AEI22_05155) neuC 985953..987071 (+) 1119 WP_052776708.1 UDP-N-acetylglucosamine 2-epimerase -
  AEI22_RS05170 (AEI22_05160) - 987068..987727 (+) 660 WP_115794552.1 acylneuraminate cytidylyltransferase family protein -
  AEI22_RS05175 (AEI22_05165) - 987724..988554 (-) 831 WP_115794553.1 glycosyltransferase -
  AEI22_RS05180 (AEI22_05170) waaV 988610..989419 (-) 810 WP_115794554.1 glycosyltransferase family 2 protein -
  AEI22_RS05185 (AEI22_05175) waaF 989478..990428 (+) 951 WP_115794555.1 lipopolysaccharide heptosyltransferase II Regulator
  AEI22_RS08445 (AEI22_05180) - 990414..991188 (-) 775 Protein_1005 glycosyltransferase family 25 protein -
  AEI22_RS05195 (AEI22_05185) gmhA 991188..991748 (-) 561 WP_115794556.1 D-sedoheptulose 7-phosphate isomerase -
  AEI22_RS05200 (AEI22_05190) rfaE1 991745..993130 (-) 1386 WP_002922733.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  AEI22_RS05205 (AEI22_05195) rfaD 993123..994076 (-) 954 WP_115794557.1 ADP-glyceromanno-heptose 6-epimerase -
  AEI22_RS05210 (AEI22_05200) gmhB 994077..994637 (-) 561 WP_002878815.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  AEI22_RS05215 (AEI22_05205) - 994719..995021 (+) 303 WP_002852762.1 cytochrome c -
  AEI22_RS05220 (AEI22_05210) ccoS 995044..995250 (-) 207 WP_002937295.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  AEI22_RS05225 (AEI22_05215) - 995247..997604 (-) 2358 WP_115794558.1 heavy metal translocating P-type ATPase -
  AEI22_RS05230 (AEI22_05220) rho 997713..999011 (+) 1299 WP_002853705.1 transcription termination factor Rho -
  AEI22_RS05235 (AEI22_05225) dnaX 999015..1000544 (+) 1530 WP_115794559.1 DNA polymerase III subunit gamma/tau -
  AEI22_RS05240 (AEI22_05230) - 1000541..1001062 (-) 522 WP_002853472.1 DUF2165 family protein -
  AEI22_RS05245 (AEI22_05235) copA 1001134..1003233 (-) 2100 WP_115794560.1 copper-translocating P-type ATPase CopA -
  AEI22_RS05250 (AEI22_05240) - 1003233..1003427 (-) 195 WP_002882808.1 cation transporter -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36274.85 Da        Isoelectric Point: 10.2875

>NTDB_id=152485 AEI22_RS05185 WP_115794555.1 989478..990428(+) (waaF) [Campylobacter jejuni strain CJ090CC1332]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKIKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHSKDLKLPFKLKFQNPLILRNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGTGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEVILKAIQNLINI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=152485 AEI22_RS05185 WP_115794555.1 989478..990428(+) (waaF) [Campylobacter jejuni strain CJ090CC1332]
ATGAAAATTTTTATACATCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCTCAATTTATCCTTTATGGCTCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCTCGCTATAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAATAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTCTAAAGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAGAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGCACAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGTGATCTTTTCATCACAAACGACAGT
GGTCCTATGCACATAAGTGCAGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACAAAATTTACCCAAACTTCACC
TTGGCAAAATGAAAACGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTGAAGTGATTTTAAAAGCAATTCAAAATTTAATTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(100-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

93.631

99.367

0.93

Multiple sequence alignment