Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   AEI15_RS05365 Genome accession   NZ_CP012214
Coordinates   1018579..1019529 (+) Length   316 a.a.
NCBI ID   WP_002884485.1    Uniprot ID   -
Organism   Campylobacter jejuni strain CJ088CC52     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1004624..1032518 1018579..1019529 within 0


Gene organization within MGE regions


Location: 1004624..1032518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AEI15_RS05300 (AEI15_05305) pglI 1004624..1005553 (-) 930 WP_002884081.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  AEI15_RS05305 (AEI15_05310) pglH 1005546..1006625 (-) 1080 WP_079264332.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  AEI15_RS05310 (AEI15_05315) pglK 1006622..1008316 (-) 1695 WP_002935022.1 ABC-type lipopolysaccharide transporter PglK -
  AEI15_RS05315 (AEI15_05320) galE 1008310..1009296 (-) 987 WP_002893768.1 UDP-glucose 4-epimerase GalE -
  AEI15_RS05320 (AEI15_05325) - 1009355..1010149 (-) 795 WP_052777648.1 3'-5' exonuclease -
  AEI15_RS05325 (AEI15_05330) waaC 1010216..1011244 (+) 1029 WP_002884086.1 lipopolysaccharide heptosyltransferase I -
  AEI15_RS05330 (AEI15_05335) htrB 1011234..1012121 (+) 888 WP_002867215.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  AEI15_RS05335 (AEI15_05340) - 1012118..1013668 (+) 1551 WP_002867216.1 glycosyltransferase -
  AEI15_RS05340 (AEI15_05345) - 1013637..1014524 (-) 888 WP_115768225.1 glycosyltransferase family 2 protein -
  AEI15_RS05345 (AEI15_05350) - 1014517..1015725 (-) 1209 WP_052847090.1 glycosyltransferase family 2 protein -
  AEI15_RS05350 (AEI15_05355) - 1015803..1016777 (-) 975 WP_100614661.1 glycosyltransferase family 2 protein -
  AEI15_RS05355 (AEI15_05360) - 1016770..1017657 (-) 888 WP_052777644.1 glycosyltransferase -
  AEI15_RS05360 (AEI15_05365) waaV 1017711..1018520 (-) 810 WP_002884487.1 glycosyltransferase family 2 protein -
  AEI15_RS05365 (AEI15_05370) waaF 1018579..1019529 (+) 951 WP_002884485.1 lipopolysaccharide heptosyltransferase II Regulator
  AEI15_RS05370 (AEI15_05375) - 1019515..1020279 (-) 765 WP_002884483.1 glycosyltransferase family 25 protein -
  AEI15_RS05375 (AEI15_05380) gmhA 1020279..1020839 (-) 561 WP_002869619.1 D-sedoheptulose 7-phosphate isomerase -
  AEI15_RS05380 (AEI15_05385) rfaE1 1020836..1022221 (-) 1386 WP_002884480.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  AEI15_RS05385 (AEI15_05390) rfaD 1022214..1023167 (-) 954 WP_052777643.1 ADP-glyceromanno-heptose 6-epimerase -
  AEI15_RS05390 (AEI15_05395) gmhB 1023168..1023729 (-) 562 Protein_1056 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  AEI15_RS05395 (AEI15_05400) - 1023810..1024112 (+) 303 WP_002852762.1 cytochrome c -
  AEI15_RS05400 (AEI15_05405) ccoS 1024135..1024341 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  AEI15_RS05405 (AEI15_05410) - 1024338..1026695 (-) 2358 WP_079264880.1 heavy metal translocating P-type ATPase -
  AEI15_RS05410 (AEI15_05415) rho 1026804..1028102 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  AEI15_RS05415 (AEI15_05420) dnaX 1028106..1029635 (+) 1530 WP_002860245.1 DNA polymerase III subunit gamma/tau -
  AEI15_RS05420 (AEI15_05425) - 1029632..1030153 (-) 522 WP_002884471.1 DUF2165 family protein -
  AEI15_RS05425 (AEI15_05430) copA 1030225..1032324 (-) 2100 WP_002884469.1 copper-translocating P-type ATPase CopA -
  AEI15_RS05430 (AEI15_05435) - 1032324..1032518 (-) 195 WP_002859258.1 hypothetical protein -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36240.79 Da        Isoelectric Point: 10.2878

>NTDB_id=152377 AEI15_RS05365 WP_002884485.1 1018579..1019529(+) (waaF) [Campylobacter jejuni strain CJ088CC52]
MKIFIHLPTWLGDAVMASPALYVIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLHIKAHLKDLKLPFKLKFQSPLVLKNGKKILGLNPGA
SFGNAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENATLVHLNLACMPCMQKTCPLKHHKCMKDLKPEVIVKAIQNLTNI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=152377 AEI15_RS05365 WP_002884485.1 1018579..1019529(+) (waaF) [Campylobacter jejuni strain CJ088CC52]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGTTATAAAAGAACA
TTTTAAAAATGCTCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTCACATAAAAGCTCACCTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAGCCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAATCCTGGTGCA
AGCTTTGGAAATGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGCCCTATGCACATAAGTGCAGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACAAAATTTACTCAAACCTCACC
TTGGCAAAATGAAAACGCAACATTAGTGCATTTAAATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTGTAAAAGCGATTCAAAATTTAACTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.332

99.051

0.915

Multiple sequence alignment