Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   AEI26_RS05870 Genome accession   NZ_CP012212
Coordinates   1110897..1111847 (+) Length   316 a.a.
NCBI ID   WP_070298520.1    Uniprot ID   -
Organism   Campylobacter jejuni strain CJ017CCUA     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1089564..1125629 1110897..1111847 within 0
IScluster/Tn 1112066..1112688 1110897..1111847 flank 219


Gene organization within MGE regions


Location: 1089564..1125629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AEI26_RS05745 (AEI26_05695) pglI 1089564..1090493 (-) 930 WP_070298424.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  AEI26_RS05750 (AEI26_05700) pglH 1090486..1091565 (-) 1080 WP_079861393.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  AEI26_RS05755 (AEI26_05705) pglK 1091562..1093256 (-) 1695 WP_070298422.1 ABC-type lipopolysaccharide transporter PglK -
  AEI26_RS05760 (AEI26_05710) galE 1093250..1094236 (-) 987 WP_002870504.1 UDP-glucose 4-epimerase GalE -
  AEI26_RS05765 (AEI26_05715) - 1094294..1095088 (-) 795 WP_070282296.1 3'-5' exonuclease -
  AEI26_RS05770 (AEI26_05720) waaC 1095154..1096182 (+) 1029 WP_070298421.1 lipopolysaccharide heptosyltransferase I -
  AEI26_RS05775 (AEI26_05725) htrB 1096172..1097050 (+) 879 WP_070298420.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  AEI26_RS05780 (AEI26_05730) - 1097047..1097748 (+) 702 WP_070298419.1 glycosyltransferase family 2 protein -
  AEI26_RS05785 (AEI26_05735) rfbA 1097759..1098637 (+) 879 WP_070298426.1 glucose-1-phosphate thymidylyltransferase RfbA -
  AEI26_RS05790 (AEI26_05740) rfbB 1098637..1099668 (+) 1032 WP_070298418.1 dTDP-glucose 4,6-dehydratase -
  AEI26_RS05795 (AEI26_05745) wlaRA 1099671..1100117 (+) 447 WP_115801387.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRA -
  AEI26_RS05800 (AEI26_05750) wlaRB 1100114..1100518 (+) 405 WP_070298416.1 class E lipooligosaccharide biosynthesis 3,4-ketoisomerase WlaRB -
  AEI26_RS05805 (AEI26_05755) - 1100508..1100951 (+) 444 WP_070298415.1 acyltransferase -
  AEI26_RS09110 (AEI26_05760) - 1101122..1101499 (+) 378 WP_353477135.1 N-acetyltransferase -
  AEI26_RS05820 (AEI26_05765) - 1101796..1102860 (+) 1065 WP_070298414.1 glycosyltransferase family A protein -
  AEI26_RS05825 (AEI26_05770) - 1102873..1103925 (+) 1053 WP_070298413.1 glycosyltransferase family 2 protein -
  AEI26_RS05830 (AEI26_05775) wlaRG 1103891..1104976 (+) 1086 WP_025998025.1 class E lipooligosaccharide biosynthesis aminotransferase WlaRG -
  AEI26_RS05835 (AEI26_05780) - 1105003..1105928 (-) 926 Protein_1132 DUF2972 domain-containing protein -
  AEI26_RS09250 (AEI26_05785) - 1106257..1107268 (-) 1012 Protein_1133 hypothetical protein -
  AEI26_RS05850 (AEI26_05790) - 1107339..1108112 (+) 774 WP_070298411.1 glycosyltransferase family 25 protein -
  AEI26_RS05855 (AEI26_05795) wlaTB 1108105..1109061 (+) 957 WP_070298410.1 class E lipooligosaccharide biosynthesis protein WlaTB -
  AEI26_RS05860 (AEI26_05800) wlaTC 1109058..1110017 (+) 960 WP_070298409.1 class E lipooligosaccharide biosynthesis protein WlaTC -
  AEI26_RS05865 (AEI26_05805) - 1110020..1110838 (-) 819 WP_070298408.1 glycosyltransferase family 2 protein -
  AEI26_RS05870 (AEI26_05810) waaF 1110897..1111847 (+) 951 WP_070298520.1 lipopolysaccharide heptosyltransferase II Regulator
  AEI26_RS05875 (AEI26_05815) - 1112066..1112685 (-) 620 Protein_1139 IS607 family transposase -
  AEI26_RS05880 (AEI26_05820) - 1112685..1113317 (-) 633 WP_070298519.1 zeta toxin family protein -
  AEI26_RS05885 (AEI26_05825) gmhA 1113413..1113985 (-) 573 WP_070298518.1 D-sedoheptulose 7-phosphate isomerase -
  AEI26_RS05890 (AEI26_05830) rfaE1 1113982..1115367 (-) 1386 WP_070286070.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  AEI26_RS05895 (AEI26_05835) rfaD 1115360..1116313 (-) 954 WP_070240085.1 ADP-glyceromanno-heptose 6-epimerase -
  AEI26_RS05900 (AEI26_05840) gmhB 1116314..1116875 (-) 562 Protein_1144 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  AEI26_RS05905 (AEI26_05845) - 1116956..1117258 (+) 303 WP_002870485.1 cytochrome c -
  AEI26_RS05910 (AEI26_05850) ccoS 1117281..1117487 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  AEI26_RS05915 (AEI26_05855) - 1117484..1119841 (-) 2358 WP_079861402.1 heavy metal translocating P-type ATPase -
  AEI26_RS05920 (AEI26_05860) rho 1119950..1121248 (+) 1299 WP_057042622.1 transcription termination factor Rho -
  AEI26_RS05925 (AEI26_05865) dnaX 1121252..1122778 (+) 1527 WP_115801389.1 DNA polymerase III subunit gamma/tau -
  AEI26_RS08990 (AEI26_05870) - 1122775..1123205 (-) 431 Protein_1150 DUF2165 family protein -
  AEI26_RS05935 (AEI26_05880) copA 1123336..1125435 (-) 2100 WP_070298516.1 copper-translocating P-type ATPase CopA -
  AEI26_RS05940 (AEI26_05885) - 1125435..1125629 (-) 195 WP_070233533.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36177.77 Da        Isoelectric Point: 10.2575

>NTDB_id=152324 AEI26_RS05870 WP_070298520.1 1110897..1111847(+) (waaF) [Campylobacter jejuni strain CJ017CCUA]
MKIFIHLPTWLGDAVMASPALYAIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLSIKAHSKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALIFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKIKTVAIFGPTKFTQTSPWQNENAKLVHSDLACMPCMQKTCPLKHHKCMKDLKPEKVIEEIKKLSTL

Nucleotide


Download         Length: 951 bp        

>NTDB_id=152324 AEI26_RS05870 WP_070298520.1 1110897..1111847(+) (waaF) [Campylobacter jejuni strain CJ017CCUA]
ATGAAAATTTTTATACATCTTCCCACTTGGTTAGGCGATGCAGTGATGGCTTCACCTGCTTTATACGCTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCTACGGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGCATATTCTCAAAACAAAACAAAGATATTTTTTTGACAAAAATAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTAGTATAAAAGCTCATTCTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAATTTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTTATCACAAATGATAGT
GGTCCTATGCACCTAAGTGCGGTTTATAAGATAAAAACCGTAGCTATTTTTGGCCCCACAAAATTTACCCAAACTTCACC
TTGGCAAAACGAAAACGCAAAATTAGTGCATTCAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAAAGGTGATAGAAGAAATTAAAAAATTAAGCACCCTATAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

95.208

99.051

0.943

Multiple sequence alignment