Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACZ75_RS16205 Genome accession   NZ_CP012201
Coordinates   3924030..3925505 (-) Length   491 a.a.
NCBI ID   WP_050409695.1    Uniprot ID   A0A0K1K0M8
Organism   Massilia sp. NR 4-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3919030..3930505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACZ75_RS16175 (ACZ75_16225) - 3919323..3919805 (+) 483 WP_050409689.1 PTS sugar transporter subunit IIA -
  ACZ75_RS16180 (ACZ75_16230) hprK 3919840..3920775 (+) 936 WP_050409690.1 HPr(Ser) kinase/phosphatase -
  ACZ75_RS16185 (ACZ75_16235) rapZ 3920951..3921811 (+) 861 WP_050409691.1 RNase adapter RapZ -
  ACZ75_RS16190 (ACZ75_16240) - 3921818..3922192 (-) 375 WP_050409692.1 VOC family protein -
  ACZ75_RS16195 (ACZ75_16245) - 3922294..3923382 (-) 1089 WP_050409693.1 enoyl-CoA hydratase/isomerase family protein -
  ACZ75_RS16200 (ACZ75_16250) hpf 3923597..3923956 (-) 360 WP_050409694.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACZ75_RS16205 (ACZ75_16255) rpoN/rpoN1 3924030..3925505 (-) 1476 WP_050409695.1 RNA polymerase factor sigma-54 Machinery gene
  ACZ75_RS16210 (ACZ75_16260) lptB 3925510..3926265 (-) 756 WP_050409696.1 LPS export ABC transporter ATP-binding protein -
  ACZ75_RS16215 (ACZ75_16265) lptA 3926268..3926855 (-) 588 WP_050409697.1 lipopolysaccharide transport periplasmic protein LptA -
  ACZ75_RS16220 (ACZ75_16270) lptC 3926881..3927474 (-) 594 WP_050409698.1 LPS export ABC transporter periplasmic protein LptC -
  ACZ75_RS16225 (ACZ75_16275) - 3927489..3928028 (-) 540 WP_050409699.1 HAD family hydrolase -
  ACZ75_RS16230 (ACZ75_16280) - 3928028..3929026 (-) 999 WP_176347855.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 54436.05 Da        Isoelectric Point: 5.0383

>NTDB_id=152174 ACZ75_RS16205 WP_050409695.1 3924030..3925505(-) (rpoN/rpoN1) [Massilia sp. NR 4-1]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDRSVRLLADGAISQTSTPGEAPPDNA
APADAPAPAAEAEPAYEGGEGESVAEADGDWGDTGRGKAPEDDDARPQLEAHHCSLREHLLEQMRVTLQEPRDRALMELI
VDALDDNGYLDESLEEIHARLPLELDVELEELQTSLTLLQSFDPAGVGARSAAECLALQIRRLPGVPLVTRRMALCIVEK
HLAWFAQREFTKLKKALDCDDEDLREAQTVIRQCNPHPGAAFAADVSDYVVPDVVVKKGRNGWIVMLNNDVMPRLRVNSL
YASLLRQGKGETQMSAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRVT
TQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREAL
KIPPVSLRKSL

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=152174 ACZ75_RS16205 WP_050409695.1 3924030..3925505(-) (rpoN/rpoN1) [Massilia sp. NR 4-1]
ATGAAACAGTCTTTGCAACTGCGCACCTCGCAACACCTCGCGTTGACGCCGCAGTTGCAGCAGTCCATCCGCCTGCTGCA
ACTGTCCACGCTGGAACTGCATCAGGAACTGGAACAGCTGCTGACTGACAATCCCCTGCTGGAGCGTCTCGACGATCCGC
TCGACCGTTCCGTGCGCCTGCTGGCCGATGGCGCCATCAGTCAGACCTCCACTCCCGGCGAAGCGCCGCCGGACAATGCC
GCGCCGGCCGATGCGCCGGCGCCGGCCGCCGAGGCCGAACCCGCCTATGAGGGCGGCGAAGGCGAGAGCGTGGCCGAAGC
CGATGGCGACTGGGGCGACACGGGCCGCGGCAAGGCGCCCGAAGACGACGACGCGCGTCCCCAGCTGGAAGCCCACCACT
GCAGCCTGCGCGAGCATTTGCTGGAACAGATGCGCGTCACCCTGCAGGAGCCGCGCGACCGCGCGCTGATGGAGCTGATC
GTCGATGCGCTGGACGACAACGGTTATCTGGACGAGAGCCTGGAAGAAATCCACGCGCGCCTGCCGCTGGAACTCGATGT
GGAGCTGGAGGAGCTGCAAACTTCGCTCACCCTGCTGCAAAGCTTCGACCCGGCCGGCGTCGGTGCGCGCAGCGCCGCCG
AATGCCTGGCGCTGCAGATCCGCCGCCTGCCCGGCGTGCCTTTGGTGACGCGGCGCATGGCTTTGTGCATCGTCGAAAAG
CACCTGGCCTGGTTCGCCCAGCGCGAATTCACCAAGCTGAAAAAAGCCCTCGACTGCGACGATGAAGACCTGCGCGAAGC
GCAGACCGTGATCCGCCAGTGCAATCCGCATCCGGGCGCCGCCTTTGCCGCCGACGTGTCGGACTACGTGGTGCCGGACG
TGGTGGTGAAGAAGGGCCGCAACGGCTGGATCGTGATGCTGAATAACGATGTGATGCCGCGCCTGCGCGTGAACTCCCTG
TACGCCAGCCTGCTGCGCCAGGGGAAGGGCGAAACGCAGATGAGCGCCCAGCTGCAGGAAGCGAAATGGCTGATCAAGAA
TATGCGCCAGCGTTTTGACACAATTTTGCGCGTGGCGCAGGCCATCGTGGAGCGTCAAAAGAATTTCTTCTCGCACGGCG
CCGTGGCCATGAGACCCCTTGTGTTGCGTGAAATTGCTGATACACTGGGACTACACGAGAGCACTATTTCTCGCGTAACA
ACTCAGAAATATATGCTCACCCCCCATGGCATGTTTGAGTTGAAATATTTTTTTGGCAGCCATGTCGCGACGGAAGCGGG
GGGCGAAGCTTCTTCCACGGCGATACGGGCGCTGATTGTGCAATTGACAGGAGCAGAAGACCCGAAAAATCCTTTATCCG
ACAGTAAGATTGCGGACATGCTGGGAGAACAAGGCATGGTAATTGCGCGACGCACTGTTGCCAAATATCGCGAAGCATTG
AAGATCCCGCCAGTCAGCCTCCGCAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-321)

Sigma-54, DNA binding domain (Sigma54_DBD)

(334-489)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1K0M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.938

100

0.635

Multiple sequence alignment