Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WM90_RS08380 Genome accession   NZ_CP015159
Coordinates   1691768..1692208 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain Eco889     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1686768..1697208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM90_RS08360 (WM90_08360) coaE 1687258..1687878 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  WM90_RS30605 - 1687903..1687947 (+) 45 WP_120795372.1 protein YacM -
  WM90_RS08365 (WM90_08365) guaC 1688103..1689146 (+) 1044 WP_001217320.1 GMP reductase -
  WM90_RS08370 (WM90_08370) hofC 1689181..1690383 (-) 1203 WP_000157289.1 protein transport protein HofC -
  WM90_RS08375 (WM90_08375) pilB 1690373..1691758 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  WM90_RS08380 (WM90_08380) pilA 1691768..1692208 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  WM90_RS08390 (WM90_08390) nadC 1692412..1693305 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WM90_RS08395 (WM90_08395) ampD 1693393..1693944 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WM90_RS08400 (WM90_08400) ampE 1693941..1694795 (+) 855 WP_000171980.1 beta-lactamase regulator AmpE -
  WM90_RS08405 (WM90_08405) aroP 1694838..1696208 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=152146 WM90_RS08380 WP_000360914.1 1691768..1692208(-) (pilA) [Escherichia coli strain Eco889]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=152146 WM90_RS08380 WP_000360914.1 1691768..1692208(-) (pilA) [Escherichia coli strain Eco889]
ATGGACAAGCAACGCGGTTTTACACTTATTGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment